Altererythrobacter amylolyticus sp. nov., isolated from lake sediment

Abstract

An aerobic, motile, Gram-stain-negative bacterium, designated strain NS1 T , was isolated from interfacial sediment from Taihu Lake, China. The strain formed yellow colonies on R2A medium. Cells were ovoid to rod-shaped and non-sporeforming. Growth occurred at 15-40 C (optimum, 28 C), at pH 5.0-10.5 (optimum, 6.5-7.5) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %). Phylogenetic trees based on 16S rRNA gene sequences showed that strain NS1 T represented a member of the genus Altererythrobacter and had the highest sequence similarity to Altererythrobacter troitsensis CCTCC AB 2015180 T (97.1 %). The average nucleotide identity value between strain NS1 T and the closest related strain based on their genomes was 78.6 %. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C 18 : 1 !7c and/ or C 18 : 1 !6c), summed feature 3 (C 16 : 1 !7c and/or C 16 : 1 !6c) and C 16 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, an unidentified glycolipid and six unidentified lipids. The genomic DNA G+C content was 66.6 mol%. On the basis of phenotypic and genotypic characteristics, strain NS1 T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter amylolyticus sp. nov. is proposed. The type strain is NS1 T (=CGMCC 1.13679 T =NBRC 113553 T).

Genomic-based taxonomic classification of the family Erythrobacteraceae

“…The family Erythrobacteraceae , belonging to the order Sphingomonadales , class Alphaproteobacteria [1], is distributed globally, inhabiting various environments including subterrestrial, lake, intertidal areas, mangrove, coastal and deep-sea sediments [2–10], soil [11–13], desert sands [14, 15], a stadium seat [16], seawater [17–19], estuary water [20–22], fresh water [23, 24], hot springs [25–27], air [28] as well as plants and animals [29–36] (Table S1, available in the online version of this article). The members of the family Erythrobacteraceae are Gram-stain-negative, rod or pleomorphic coccoid-shaped, pink-, red-, orange- or yellow-pigmented, and aerobic chemoorganotrophs [1].…”

“…[38], Liu et al [30], Xu et al [4] and Feng et al [2], respectively, based on 16S rRNA gene phylogeny [2, 4, 30, 38]. At the time of writing (September 2019), the genera Altererythrobacter , Croceibacterium , Croceicoccus , Erythrobacter , Erythromicrobium , Porphyrobacter and Qipengyuania consist of 41, two, four, 23, one, eight and one species, respectively [9, 10, 17, 19, 22, 30, 35, 39–43]. With the increase in the number of species proposed, the taxonomic status of the family Erythrobacteraceae should be revised in view of the polyphyletic nature of the group based on 16S rRNA gene sequence comparison [16, 30, 44].…”

Rufibacter hautae sp. nov., a red-pigmented bacterium from freshwater lake sediment, and proposal of Rufibacter quisquiliarum as a latter heterotypic synonym of Rufibacter ruber

“…Motility was assessed in semi-solid R2A containing 0.4 % agar. Temperature, pH and salt tolerance tests were carried out in R2A broth, at 4, 10, 15, 20, 25, 28, 37 and 45 °C, at pH 3 to 12 (at increments of 0.5 pH units) and with NaCl concentrations from 0–4 % (w/v, with intervals of 0.5 %), respectively, as described by Qu et al [25]. Oxidase activity was examined by 1 % (w/v) tetramethyl-p-phenylenediamine.…”

Pseudopontixanthobacter vadosimaris gen. nov., sp. nov., isolated from shallow sea near Kueishan Island

“…Different methanol-soluble pigments were detected, and NaCl was found to be a species-dependent growth requirement within members of the family Erythrobacteraceae . The members have been isolated from marine environments [10, 11], fresh water [12, 13], sand [14], air [15], soil [16], hot spring [17] and some organisms [18, 19]. This diversity of habitats suggests the wide distribution of the family members.…”