Altered rumen fermentation patterns in lactating dairy cows supplemented with phytochemicals improve milk production and efficiency

Grazziotin, Rodrigo Chaves Barcellos; Halfen, J.; Rosa, Fernanda; Schmitt, Eduardo; Anderson, J. L.; Ballard, V.; Osorio, J. S.

doi:10.3168/jds.2019-16996

Cited by 28 publications

(28 citation statements)

References 55 publications

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“…The 4-fold-dilution standard curve was created using standardized DNA from all samples. The qPCR reactions were performed with the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using the same conditions described by Grazziotin et al (2020). A geometrical mean of 2 universal primers was used to calculate the relative abundance of bacterial species with the efficiency-corrected ∆-CT method (Abdelmegeid et al, 2018).…”

Section: Ruminal Bacteria Dna Isolation and Qpcr Amplification Of 16s...mentioning

confidence: 99%

Effects of yeast culture supplementation on lactation performance and rumen fermentation profile and microbial abundance in mid-lactation Holstein dairy cows

Halfen

Carpinelli

Pino

et al. 2021

Journal of Dairy Science

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The continuous trend for a narrowing margin between feed cost and milk prices across dairy farms in the United States highlights the need to improve and maintain feed efficiency. Yeast culture products are alternative supplements that have been evaluated in terms of milk performance and feed efficiency; however, less is known about their potential effects on altering rumen microbial populations and consequently rumen fermentation. Therefore, the objective of this study was to evaluate the effect of yeast culture supplementation on lactation performance, rumen fermentation profile, and abundance of major species of ruminal bacteria in lactating dairy cows. Forty mid-lactation Holstein dairy cows (121 ± 43 days in milk; mean ± standard deviation; 32 multiparous and 8 primiparous) were used in a randomized complete block design with a 7-d adaptation period followed by a 60-d treatment period. Cows were blocked by parity, days in milk, and previous lactation milk yield and assigned to a basal total mixed ration (TMR; 1.6 Mcal/kg of dry matter, 14.6% crude protein, 21.5% starch, and 38.4% neutral detergent fiber) plus 114 g/d of ground corn (CON; n = 20) or basal TMR plus 100 g/d of ground corn and 14 g/d of yeast culture (YC; n = 20; Culture Classic HD, Cellerate Yeast Solutions, Phibro Animal Health Corp.). Treatments were top-dressed over the TMR once a day. Cows were individually fed 1 × /d throughout the trial. Blood and rumen fluid samples were collected in a subset of cows (n = 10/treatment) at 0, 30, and 60 d of the treatment period. Rumen fluid sampled via esophageal tubing was analyzed for ammonia-N, volatile fatty acids (VFA), and ruminal bacteria populations via quantita-tive PCR amplification of 16S ribosomal DNA genes. Milk yield was not affected by treatment effects. Energy balance was lower in YC cows than CON, which was partially explain by the trend for lower dry matter intake as % body weight in YC cows than CON. Cows fed YC had greater overall ruminal pH and greater total VFA (mM) at 60 d of treatment period. There was a contrasting greater molar proportion of isovalerate and lower acetate proportion in YC-fed cows compared with CON cows. Although the ruminal abundance of specific fiber-digesting bacteria, including Eubacterium ruminantium and Ruminococcus flavefaciens, was increased in YC cows, others such as Fibrobacter succinogenes were decreased. The abundance of amylolytic bacteria such as Ruminobacter amylophilus and Succinimonas amylolytica were decreased in YC cows than CON. Our results indicate that the yeast culture supplementation seems to promote some specific fiber-digesting bacteria while decreasing amylolytic bacteria, which might have partially promoted more neutral rumen pH, greater total VFA, and isovalerate.

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Section: Ruminal Bacteria Dna Isolation and Qpcr Amplification Of 16s...mentioning

confidence: 99%

Effects of yeast culture supplementation on lactation performance and rumen fermentation profile and microbial abundance in mid-lactation Holstein dairy cows

Halfen

Carpinelli

Pino

et al. 2021

Journal of Dairy Science

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“…The bioactive compounds (phenolics and essential oils) present in the supplemented feed additive might have improved rumen function for efficient utilization of nutrients. In a study with dietary supplementation of phytochemicals (tannins and Capsicum species) to lactating dairy cows, Grazziotin et al 38 . observed increased milk production and efficiency owing to higher volatile fatty acids and lower ruminal abundance of bacteria ( Selenomonas ruminantium , Succinimonas amylolytica and Streptococcus bovis ) associated with low feed efficiency.…”

Section: Discussionmentioning

confidence: 99%

Influence of dietary phytogenic feed additives on lactation performance, methane emissions and health status ofMurrah buffaloes (Bubalus bubalis)

et al. 2021

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BACKGROUND Several feed additives have been used in recent past to reduce enteric methane production in ruminants. But most of them also inhibit feed digestibility and rumen fermentation, thereby lowering animal performance. Phytogenic feed additives are gaining importance owing to their safety in regard to human health issues. The present study examined the effect of dietary supplementation of phytogenic feed additive containing a blend of poplar (Populus deltoides) and eucalyptus (Eucalyptus citriodora) leaves on feed utilization, milk production, methane emissions and health status of Murrah buffaloes (Bubalus bubalis). RESULTS The daily milk yield, 6% fat corrected milk yield, and fat–protein corrected milk yield were increased (P < 0.05) in phytogenic composite feed additive (PCFA)‐supplemented buffaloes. A decrease (37.3%) in methane concentration in exhaled air of supplemented buffaloes was evident. The digestibility coefficient of dry matter, organic matter and neutral detergent fibre was increased (P < 0.05) in PCFA‐fed buffaloes without affecting feed intake. Total digestible nutrient content of the ration fed to buffaloes of the PCFA group was significantly (P < 0.05) increased. The buffaloes fed PCFA exhibited an enhanced cell‐mediated and humoral immune response. CONCLUSION A discernible positive impact was evident on overall performances and health status along with lowered methane production of buffaloes fed (15 g kg−1 dry matter intake) a blend of phytogenic feed additive composed of leaves of poplar (P. deltoides) and eucalyptus (E. citriodora). © 2021 Society of Chemical Industry

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“…Broderick et al (64) suggested that silage containing 0.5% of CT improved milk yield and milk protein. Tannins have the potential to increase RUP in a diet, and when combined with high-quality protein that contains a significant amount of limiting amino acid like methionine and lysine, this could lead to an improvement in N efficiency and milk production by increasing the flow of an adequate supply of amino acid to the small intestine (11). In addition, Polyorach et al (24) found that mangosteen peel supplementation could enhance DM intake and digestibility of nutrients and improve the fermentation process, milk yield, and composition in lactating dairy cows.…”

Section: Milk Yield and Compositionsmentioning

confidence: 99%

“…These methods can increase RUP fraction of SBM up to 70% (8,9). However, the use of phytonutrient to protect protein sources and enhance protein utilization and ruminant performance has very limited information (10,11). Because of their natural characteristics as compared to chemical additives, phytonutrients condensed tannins (CTs) and saponins (SPs) are important ruminant feed additives, in particular for use as a CH 4 mitigation strategy and to improve the rumen volatile fatty acid (VFA) profiles (12,13).…”

Section: Introductionmentioning

confidence: 99%

Mangosteen Peel Liquid-Protected Soybean Meal Can Shift Rumen Microbiome and Rumen Fermentation End-Products in Lactating Crossbred Holstein Friesian Cows

Phesatcha

Wanapat

2022

Front. Vet. Sci.

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Rumen bypass protein can enhance protein availability in the lower gut. This study investigated the use of liquid-containing phytonutrients in dairy cows as a dietary additives to reduce rumen protein degradation. Four crossbred lactating Holstein Friesian cows (75% Holstein Friesian with 25% Thai native breed) with an initial body weight (BW) of 410 ± 20 kg were randomly assigned to a 2 × 2 factorial arrangement [two crude protein (CP) levels with soybean meal (SBM) or mangosteen peel liquid-protected soybean meal (MPLP)-SBM] in a 4 × 4 Latin square design experiment. Dietary treatments were as follows: T1 = SBM in low crude protein concentrate (LPC) (SBM-LPC); T2 = MPLP-SBM in LPC (MPLP-SBM-LPC); T3 = SBM in high crude protein concentrate (HPC) (SBM-HPC); T4 = MPLP-SBM in HPC (MPLP-SBM-HPC). Apparent digestibilities of organic matter (OM) and neutral detergent fiber (aNDF) were increased (p < 0.05) by CP level in the HPC diet (19% CP), with higher OM and aNDF digestibilities. High crude protein concentrate increased (p < 0.05) the propionic acid in the rumen but reduced (p < 0.05) the acetic acid-to-propionic acid ratio and methane (CH4) production. Rumen microbial populations of the total bacteria, Fibrobacter succinogenes and Butyrivibrio fibrisolvens were increased (p < 0.05) by HPC. Real-time PCR revealed a 30.6% reduction of rumen methanogens by the MPLP-SBM in HPC. Furthermore, efficiency of microbial nitrogen synthesis (EMNS) was 15.8% increased (p < 0.05) by the MPLP-SBM in HPC when compared to SBM-LPC. Milk yield and milk composition protein content were enhanced (p < 0.05) by both the CP level in concentrate and by MPLP inclusion. In this experiment, a high level of CP and the MPLP-SBM enhanced the ruminal propionate, shifted rumen microbiome, and enhanced milk yield and compositions.

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Altered rumen fermentation patterns in lactating dairy cows supplemented with phytochemicals improve milk production and efficiency

Cited by 28 publications

References 55 publications

Effects of yeast culture supplementation on lactation performance and rumen fermentation profile and microbial abundance in mid-lactation Holstein dairy cows

Effects of yeast culture supplementation on lactation performance and rumen fermentation profile and microbial abundance in mid-lactation Holstein dairy cows

Influence of dietary phytogenic feed additives on lactation performance, methane emissions and health status ofMurrah buffaloes (Bubalus bubalis)

Mangosteen Peel Liquid-Protected Soybean Meal Can Shift Rumen Microbiome and Rumen Fermentation End-Products in Lactating Crossbred Holstein Friesian Cows

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