Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice

Abstract

Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice.

An exploratory approach to identify microRNAs as circulatory biomarker candidates for epilepsy-associated psychiatric comorbidities in an electrical post-status epilepticus model

“…More precisely, there is convincing evidence that seizure frequency in patients with epilepsy can correlate with depression and anxiety disorders in a relevant manner (e.g. [41][42][43] ). As a consequence of the last validation step, the removal of microRNAs with Ct-values > 30 increased the risk of missing CNS-derived microRNAs whose intrinsic concentration is low, which should be considered a limitation in the context of sensitivity.…”

Complete blood count and selected serum parameters of Venus transgenic rabbits

“…2020). The UBC-GFP transgene was integrated into a noncoding region on mouse chromosome 17, but the position effect, as a reason of defect in lymphopoiesis cannot be ruled out completely (Liu et al . 2020; Necas et al .…”

“…Excessive Venus fluorescence was not observed in the bone marrow of CAG-Venus rabbits (data not shown). In CAG-EGFP mice, total body X-ray irradiation led to increased apoptosis in the bone marrow cells, compared with WT mice (Liu et al . 2020).…”