Altered representation of naive and memory CD8 T cell subsets in HIV-infected children.

Abstract

CD8 T cells are divided into naive and memory subsets according to both function and phenotype. In HIlV-negative children, the naive subset is present at high frequencies, whereas memory cells are virtually absent. Previous studies have shown that the overall number of CD8 T cells does not decrease in HIV-infected children. In studies here, we use multiparameter flow cytometry to distinguish naive from memory CD8 T cells based on expression of CD11a, CD45RA, and CD62L. With this methodology, we show that within the CD8 T cell population, the naive subset decreases markedly (HIV + vs. HIV -, 190 vs. 370 cells/,lp; P < 0.003), and that there is a reciprocal increase in memory cells, such that the total CD8 T cell counts remained unchanged (800 vs. 860 cells/pul; P < 0.76). In addition, we show that for HIV-infected children, the naive CD8 T cell and total CD4 T cell counts correlate (k P 0.001). This correlated loss suggests that the loss of naive CD8 T cells in HIV infection may contribute to the defects in cell-mediated immunity which become progressively worse as the HIV disease progresses and CD4 counts decrease. (J. Clin. Invest. 1995.

Expression of Werner and Bloom syndrome genes is differentially regulated by in vitro HIV-1 infection of peripheral blood mononuclear cells

“…These findings support the view that the levels of WRN, BLM and RecQL1 helicases are crucial to guarantee genomic stability in actively proliferating and/or transformed cells [11]. Multiple experimental evidences suggested analogies of T cell dynamics between advanced ageing and HIV infection [19][20][21][22][23][24][25][26][27][28][29][30]. Recently, it has been suggested that through a process of continuous immune activation HIV-1 infection leads to an acceleration in the ageing process of the adaptive immune system, thus contributing to immunodeficiency [31].…”

“…+ subset [19][20][21][22][23] expansion of CD8 + CD28 -T cells [24], as well as the restriction of the CD8 + T cell repertoire [25,26] suggest a typical perturbation of the T cell subpopulations that is seen in both HIV disease and advanced ageing. Moreover, a decreasing number of recent thymic emigrants, identified as T cell receptor excision circles (TRECs) [27][28][29], have been observed with ageing and in HIVinfected individuals, while highly active antiretroviral therapy (HAART) treatment leads to an increased thymic output [29,30].…”

Perforin-low memory CD8+cells are the predominant T cells in normal humans that synthesize the β-chemokine macrophage inflammatory protein-1β

“…The synthesis of MIP-1␤ by naive and memory subsets was investigated by using CD45R0 and CD62L to define naive (CD45R0 Ϫ CD62L ϩ ), M1 (CD45R0 ϩ CD62L Ϫ ), M2 (CD45R0 ϩ CD62L ϩ ), and M3 (CD45R0 Ϫ CD62L Ϫ ) subsets similar to those described by Roederer and coworkers (36,37) (Fig. 6a).…”

Reduced naive and increased activated CD4 and CD8 cells in healthy adult Ethiopians compared with their Dutch counterparts

“…Loss of CD4 þ T cells is known to be the hallmark of HIV infection and it is a well accepted laboratory marker of HIV disease progression [6,7]. Also, changes in other T cell subsets have been implicated in HIV þ persons from studies in Europe and North America: expansion of CD8 þ T cells [8], loss of naive CD8 þ T cells, increase of both CD4 þ and CD8 þ T cell memory subsets [9,10] and increased activated CD4 þ and CD8 þ T cell subsets [11][12][13][14][15][16].…”