Altered redox status in patients with Diabetes Mellitus type I

Valle, Lizette Gil del; Milian, Lazara de la C; Toledo, Arturo; Vilaró, Nora; Tápanes, Rolando; Otero, María Ángeles

doi:10.1016/j.phrs.2004.10.012

Cited by 42 publications

(14 citation statements)

References 36 publications

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“…52,53 Dysregulated ROS homeostasis or signaling responses have been reported in a variety of human skeletal muscular dystrophies, 53 cancers, 54 neurodegeneration, 15 obesity, 55 and, increasingly, metabolic disease such as diabetes. 56 The redox proteomic approach developed in the present study could be readily applied to these disorders to identify metabolic pathways that are affected. This technique provides meaningful information regarding the overall proteome and both the sensitivity and oxidation states of individual cysteine residues.…”

Section: Discussionmentioning

confidence: 99%

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

et al. 2014

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The molecular mechanisms underlying skeletal muscle aging and associated sarcopenia have been linked to an altered oxidative status of redox-sensitive proteins. Reactive oxygen and reactive nitrogen species (ROS/RNS) generated by contracting skeletal muscle are necessary for optimal protein function, signaling, and adaptation. To investigate the redox proteome of aging gastrocnemius muscles from adult and old male mice, we developed a label-free quantitative proteomic approach that includes a differential cysteine labeling step. The approach allows simultaneous identification of up- and downregulated proteins between samples in addition to the identification and relative quantification of the reversible oxidation state of susceptible redox cysteine residues. Results from muscles of adult and old mice indicate significant changes in the content of chaperone, glucose metabolism, and cytoskeletal regulatory proteins, including Protein DJ-1, cAMP-dependent protein kinase type II, 78 kDa glucose regulated protein, and a reduction in the number of redox-responsive proteins identified in muscle of old mice. Results demonstrate skeletal muscle aging causes a reduction in redox-sensitive proteins involved in the generation of precursor metabolites and energy metabolism, indicating a loss in the flexibility of the redox energy response. Data is available via ProteomeXchange with identifier PXD001054.

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Section: Discussionmentioning

confidence: 99%

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

et al. 2014

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“…Many studies have shown that the antioxidant capacity of serum from DM patients is significantly reduced compared with that of healthy individuals [26, 27]. It can be inferred, therefore, that the antioxidant capacity of urine from diabetics will be lower than that of urine from healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Method for Measuring Antioxidant Capacity in Urine, Based on Oxidation Reduction Potential and Redox Couple I₂/KI

Cao

Bai

et al. 2016

Bioinorganic Chemistry and Applications

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Objectives. To establish a new method for determination of antioxidant capacity of human urine based on the redox couple I2/KI and to evaluate the redox status of healthy and diseased individuals. Methods. The method was based on the linear relationship between oxidation reduction potential (ORP) and logarithm of concentration ratio of I2/KI. ORP of a solution with a known concentration ratio of I2/KI will change when reacted with urine. To determine the accuracy of the method, both vitamin C and urine were reacted separately with I2/KI solution. The new method was compared with the traditional method of iodine titration and then used to measure the antioxidant capacity of urine samples from 30 diabetic patients and 30 healthy subjects. Results. A linear relationship was found between logarithm of concentration ratio of I2/KI and ORP (R 2 = 0.998). Both vitamin C and urine concentration showed a linear relationship with ORP (R 2 = 0.994 and 0.986, resp.). The precision of the method was in the acceptable range and results of two methods had a linear correlation (R 2 = 0.987). Differences in ORP values between diabetic group and control group were statistically significant (P < 0.05). Conclusions. A new method for measuring the antioxidant capacity of clinical urine has been established.

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“…AOPP have been widely accepted into the field of oxidative stress research, as a marker of plasma protein oxidative damage [8–10, 12, 15, 17, 18, 22, 23, 31, 35, 38, 41, 44, 48–51]. However, on thorough study of the literature, it is apparent that two discrete versions of the AOPP assay exist which both, at first sight, resemble an assay to measure chloramines, in which the oxidation of iodide (I − ) to triiodide (I 3 − ) by phagocyte-derived chloramines is measured [52].…”

Section: Discussionmentioning

confidence: 99%

“…In yet further studies on AOPP in diabetes, it was unclear which protocol was followed [15, 18, 51]. Differences in methodology may partially explain the discrepancy in AOPP values published in previous studies of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus

Taylor

Armstrong

Perrett

et al. 2015

Oxidative Medicine and Cellular Longevity

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Advanced oxidation protein products (AOPP) are reportedly elevated in the plasma of patients with a number of diseases, including diabetes mellitus, that involve oxidative stress. However, the accurate measurement of AOPP in human plasma is hampered by the formation of a precipitate following the addition of potassium iodide and glacial acetic acid according to the published assay procedure. Here we describe a modification of the AOPP assay which eliminates interference by precipitation and provides a robust, reliable, and reproducible protocol for the measurement of iodide oxidising capacity in plasma samples (intra-assay CV 1.7–5.3%, interassay CV 5.3–10.5%). The improved method revealed a significant association of AOPP levels with age (p < 0.05) and hypertension (p = 0.01) in EDTA-anticoagulated plasma samples from 52 patients with diabetes and 38 nondiabetic control subjects, suggesting a possible link between plasma oxidising capacity and endothelial and/or vascular dysfunction. There was no significant difference between AOPP concentrations in diabetic (74.8 ± 7.2 μM chloramine T equivalents) and nondiabetic (75.5 ± 7.0 μM chloramine T equivalents) individuals.

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Altered redox status in patients with Diabetes Mellitus type I

Cited by 42 publications

References 36 publications

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

Establishment of a Method for Measuring Antioxidant Capacity in Urine, Based on Oxidation Reduction Potential and Redox Couple I₂/KI

Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus

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Altered redox status in patients with Diabetes Mellitus type I

Cited by 42 publications

References 36 publications

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging

Establishment of a Method for Measuring Antioxidant Capacity in Urine, Based on Oxidation Reduction Potential and Redox Couple I2/KI

Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus

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Establishment of a Method for Measuring Antioxidant Capacity in Urine, Based on Oxidation Reduction Potential and Redox Couple I₂/KI