Altered plasma albumin characteristics and lymphocyte populations in gastrointestinal cancer patients: Detection using modified fluorescence responses

Kalnina, Inta; Kirilova, Elena; Klimkane, Laura; Kirilov, G

doi:10.3109/15476910903280090

Cited by 7 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many questions regarding structure and properties of albumin remain unclear, among them physical properties related to albumin structure (especially in molecule tryptophan area), functional role of ligand and transportation of oxidation products during various pathologies. The reason for examining albumin is that this protein is practically the only source of ABM binding in blood plasma with a very high selectivity and subsequent fluorescence [5,13,15].…”

Section: Discussionmentioning

confidence: 99%

“…Probe ABM was approbated on patients with gastrointestinal cancer [13,15] and humans exposed to ionizing radiation during the clean-up work in Chernobyl [8,9]. In groups of Chernobyl clean-up workers, the patterns in spectral characteristics of ABM resemble the so-called N-F transition of albumin with the blue shift of fluorescence maximum to 602-620 nm and increased fluorescence intensity as compared to the healthy control group.…”

Section: Discussionmentioning

confidence: 99%

“…There is strong correlation between ABM fluorescence intensity in blood plasma and level of tryptophanyl region dehydration of albumin molecule [15]. The shifts of fluorescence zone to the short-wave region and decreased fluorescence intensity in Chernobyl clean-up workers provide evidence of hydration of tryptophanyl region of the albumin molecule [4,12].…”

Section: Albumin Auto-fluorescencementioning

confidence: 99%

See 2 more Smart Citations

Radiation-modified albumin in type 2 diabetic patients

Kalnina¹,

Kirilova²,

Zvagule³

et al. 2013

J Exp Integr Med

Self Cite

View full text Add to dashboard Cite

Objectives: Diabetes mellitus has been one of the most crippling diseases that man has seen, and its prevalence has risen dramatically over the past two decades. Currently, there are over 150 million diabetics worldwide and this number is likely to increase to 300 million or more by the year 2025. Diabetes mellitus increases the risk of many disorders including cardiovascular diseases. Understanding the molecular properties of diabetic progression is a big challenge in the system biology era. The aim of this study is to determine association in albumin modifications between Chernobyl clean-up workers with and without type 2 diabetes mellitus. Methods: ABM (3-aminobenzanthrone derivative developed in Daugavpils University, Latvia) has been previously shown as a potential biomarker for determination of the immune state of patients with different pathologies. In this study several aspects of plasma albumin alterations in the group of Chernobyl clean-up workers with diabetes mellitus in relation to the group of workers without diabetes mellitus and people having no professional contact with radioactivity were determined. The following parameters were examined: (1) spectral characteristics of ABM in blood plasma; (2) effective and total albumin concentration in blood plasma; and (3) quantitative parameters of albumin auto-fluorescence. Results: Screening of individuals with diabetes mellitus 25-26 years after their work in Chernobyl revealed two groups of patients differing in structural and functional properties of membrane. The revealed structural modifications of membrane are dependent on radiation-induced factors. Concomitant diseases (diabetes mellitus, cardiovascular diseases) reinforce radiation-induced effects. Conclusion: ABM is a sensitive probe of albumin alterations; it can be used to elucidate the changes in protein systems. Significant differences in albumin dynamics exist between the control group (donors) and that of diabetics and non-diabetics in the Chernobyl clean-up workers. [J Exp Integr Med 2013; 3(1): 3-7

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Albumin Auto-fluorescencementioning

confidence: 99%

See 1 more Smart Citation

Radiation-modified albumin in type 2 diabetic patients

Kalnina¹,

Kirilova²,

Zvagule³

et al. 2013

J Exp Integr Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…The above mentioned changes seem to be useful in reflecting alterations within the lymphocytes of cancer patients in each sub-group before and after operation; they change as a function of cancer staging and therefore may ultimately be of use as potential indices of altered cellular immunity in these hosts [9][10][11][12].…”

Section: Discussionmentioning

confidence: 99%

“…This work was performed using a novel fluorescent probe ABM (derivative of benzanthrone) as a biomarker. Synthesis and properties of this probe are described in our previous works [9][10][11][12]. Our aim was to elaborate a new fluorescent-based method for use in immunological-based diagnostics of gastrointestinal cancer (e.g., colorectal) patients.…”

Section: Introductionmentioning

confidence: 99%

Blood albumin alterations and lymphocyte populations in advanced cancer patients

Kalnina¹,

Kurjāne²,

Kirilova³

et al. 2011

J Exp Integr Med

Self Cite

View full text Add to dashboard Cite

Patients with cancer (namely advanced cancer) exhibit poorly functionating immune system. It is now widely accepted that dynamics of changes, along the certain types of alterations in structures of lipid/protein themselves of immune system cells and blood plasma, plays a critical role in the maintenance of the immune status of organism. Biomarkers for prediction of disease outcome are of great interest in human medicine. Methods: The fluorescent probe ABM was used to characterize lymphocyte membranes and blood plasma albumin of cancer patients suffering from advanced cancer with wide metastasis and intoxication. The aim of these studies was to evaluate the potential applicability of measures of ABM fluorescence intensity as a standard tool in the analyses of host immune status and for a clinical interpretation of alterations in albumin per se and in lymphocyte functional activity in cancer patients receiving palliative care. We registered probe ABM spectral parameters in patients" lymphocytes and blood plasma, and ABM auto-fluorescence in plasma. Results: The fluorescence intensity of ABM in blood plasma and lymphocyte suspension differed from control values and showed specific differences in patient groups in accordance (correlation) with survival rate. A significant decrease in ABM fluorescence in plasma could be explained, in part, by a diminished binding capacity of the albumin of these patients. The lymphocyte distribution among the subsets of patients also differed. Interestingly, the ABM fluorescence in the cell suspension and blood plasma was also found to correlate with selected immunological parameters (CD4 + :CD8 + ratios, lymphocyte counts, etc.). Conclusion: Results strongly correlated with changes in ABM spectral characteristics and both clinical and pathological estimates of patient immune state. ABM spectroscopy appears to be useful for clinicians to monitor the course of certain diseases (e.g., gastrointestinal cancers).

show abstract