Altered Phospholipase Activities Related to α1-Adrenergic Receptor Supersensitivity of Aortas from Aldosterone-Salt Hypertensive Rats

Jones, Allan W.; Shukla, Shivendra D.; Geisbuhler, Brinda B.; Jones, Susan B.; Smith, Jacquelyn M.

doi:10.1007/978-1-4684-6015-5_5

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…Several bodies of evidence suggest that PLA 2 -induced NE increase is independent of cellular calcium, as NE-induced PLA 2 is independent of cellular calcium activity [64]. This action is mediated by the α1-adrenergic receptor [65].…”

Section: Pla 2 and Ne Functionmentioning

confidence: 99%

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

Folley

Doop

Park

2003

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Recent evidence suggests that genetic and biochemical factors associated with psychoses may also provide an increased propensity to think creatively. The evolutionary theories linking brain growth and diet to the appearance of creative endeavors have been made recently, but they lack a direct link to research on the biological correlates of divergent and creative thought. Expanding upon Horrobin's theory that changes in brain size and in neural microconnectivity came about as a result of changes in dietary fat and phospholipid incorporation of highly unsaturated fatty acids, we propose a theory relating phospholipase A 2 (PLA 2 ) activity to the neuromodulatory effects of the noradrenergic system. This theory offers probable links between attention, divergent thinking, and arousal through a mechanism that emphasizes optimal individual functioning of the PLA 2 and NE systems as they interact with structural and biochemical states of the brain. We hope that this theory will stimulate new research in the neural basis of creativity and its connection to psychoses.

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Section: Pla 2 and Ne Functionmentioning

confidence: 99%

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

Folley

Doop

Park

2003

Prostaglandins, Leukotrienes and Essential Fatty Acids

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α₁‐ADRENOCEPTORS ON RABBIT AORTIC SMOOTH MUSCLE CELLS IN CULTURE AND IN EXPERIMENTAL INTIMAL THICKENING

Manderson

Hayward

Pak

et al. 1995

Clin Exp Pharma Physio

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1. This study has defined alpha 1-adrenoceptors and their reactivity in rabbit aorta, following removal of the endothelium and formation of a myointimal thickening, and also in smooth muscle cells (SMC) in cell culture which had undergone serial passaging and changes in phenotype. 2. [3H]-prazosin binding to SMC from control aorta, vessels 2 weeks after endothelial denudation and sub-cultured SMC (passage 3-6) was specific (displaceable with 10 mumol/L phentolamine), and of high affinity to a single class of sites (KD range: 71-114 pmol/L). The maximum binding density (Bmax) of alpha 1-adrenoceptors on SMC from the neointima (11,105 +/- 771 sites/cell) was not significantly different to that of control medial SMC (14,014 +/- 2472 sites/cell). However, SMC cultured to passage 6, showed a 2-fold increase in Bmax (30,227 +/- 4349 sites/cell). 3. The production of inositol phosphates (IP1, IP2 and IP3) by SMC following 10 mumol/L phenylephrine was assayed. Both freshly-dispersed aortic SMC and sub-cultured SMC were stimulated to produce increased inositol phosphates by the addition of phenylephrine which was completely inhibited by pre-incubation with 10 mumol/L phentolamine, suggesting that the stimulation was via alpha 1-adrenoceptors. 4. Maximal contractile responses of isolated thoracic and abdominal aortic rings to KCl (100 mmol/L), 5-HT and phenylephrine were unchanged two weeks after endothelial denudation. However, phenylephrine was significantly less potent (2.7-fold) in both areas of the aorta, while the potency of 5-HT was significantly enhanced (2.7-fold) after endothelial denudation only in the abdominal aorta. 5. The decreased sensitivity of the rabbit aorta to alpha 1-adrenoceptor agonists following endothelial denudation and the formation of a myointimal thickening is not due to changes in affinity or density of alpha 1-adrenoceptors. However multiple passaging of SMC in culture leads to an increase in alpha 1-adrenoceptor density. This change can be related to the altered cytodifferentiation of irreversible synthetic state SMC which are similar to those in atherosclerotic lesions.

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Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta

Jones

Shukla

Geisbuhler

1993

American Journal of Physiology-Cell Physiology

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We sought to relate norepinephrine (NE) stimulation of phosphatidic acid (PA) production to functional responses of rat aorta and pathways for PA production. The time course for changes in PA was closely related to Ca-dependent tonic responses in 42K efflux and contraction. NE (30 microM for 1 min) increased PA and reduced phosphatidylcholine (PC) and phosphatidylinositol (PI) based on Pi analyses and 32P labeling of phospholipids. The 32P-to-Pi ratio in PA (0.8 +/- 0.2, n = 13) was similar to PC (0.8 +/- 0.1, n = 14) but was significantly lower (P < 0.001) than PI (4.6 +/- 0.5, n = 14). The 32P-to-Pi ratio in PA was also lower (P < 0.02) than phosphatidylinositol phosphate and phosphatidylinositol bisphosphate. NE also increased [3H]PA twofold (P < 0.05) when PC was selectively labeled with [3H]myristic acid. These observations are more consistent with PA being formed from the hydrolysis of PC by phospholipase D (PLD) than by the phosphorylation of diacylglycerol produced by the action of phospholipase C. PLD was assayed by the formation of phosphatidylethanol (PEt) via a transphosphatidylation reaction with ethanol (half-maximal stimulation at 0.4-0.5% vol/vol). The time course for PLD stimulation by NE was similar to PA, with significant increases (P < 0.002) during 10 s to 30 min exposure. Once formed, PEt was degraded slowly, with a half time > 3 h. It is concluded that NE stimulates PLD in rat aorta, which forms a significant amount of PA from the hydrolysis of PC.(ABSTRACT TRUNCATED AT 250 WORDS)

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Altered Phospholipase Activities Related to α1-Adrenergic Receptor Supersensitivity of Aortas from Aldosterone-Salt Hypertensive Rats

Cited by 5 publications

References 25 publications

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

α₁‐ADRENOCEPTORS ON RABBIT AORTIC SMOOTH MUSCLE CELLS IN CULTURE AND IN EXPERIMENTAL INTIMAL THICKENING

Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta

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Altered Phospholipase Activities Related to α1-Adrenergic Receptor Supersensitivity of Aortas from Aldosterone-Salt Hypertensive Rats

Cited by 5 publications

References 25 publications

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

Psychoses and creativity: is the missing link a biological mechanism related to phospholipids turnover?

α1‐ADRENOCEPTORS ON RABBIT AORTIC SMOOTH MUSCLE CELLS IN CULTURE AND IN EXPERIMENTAL INTIMAL THICKENING

Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta

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Product

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α₁‐ADRENOCEPTORS ON RABBIT AORTIC SMOOTH MUSCLE CELLS IN CULTURE AND IN EXPERIMENTAL INTIMAL THICKENING