Altered Phage P1 Attachment to Strains of Escherichia Coli Carrying the Plasmid ColV,I-K94

Goodson, Margaret; Rowbury, R. J.

doi:10.1099/0022-1317-68-7-1785

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Another issue with P1 transduction is that some recipient or donor strains are resistant to P1 infection and therefore cannot be engineered by P1 transduction [4]. However, a key drawback of P1 transduction for complex strain development is that the phage package and transfers approximately 100 kb of donor DNA.…”

Section: Introductionmentioning

confidence: 99%

Strain Engineering by Genome Mass Transfer: Efficient Chromosomal Trait Transfer Method Utilizing Donor Genomic DNA and Recipient Recombineering Hosts

2009

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Strain engineering, like cloning, is a fundamental technology used to confer new traits onto existing strains. While effective methods for trait development through gene modification within strains have been developed, methods for trait transfer between Escherichia coli strains to create complex strains are needed. We report herein the development of genome mass transfer (GMT), a broadly applicable new strain engineering methodology enabling rapid trait transfer from a donor strain into a recombineering gene-expressing recipient strain. GMT utilizes electroporation of donor chromosomal DNA into a recombineering recipient cell for precise trait transfer. GMT transfer of traits between E. coli strains can be used to rapidly assemble new strains incorporating combinations of marked gene knockouts, for example, utilizing the existing E. coli K-12 Keio gene knockout collection as source target genes. Optional use of random primed isothermal amplified DNA eliminates the need for initial DNA purification, affording high throughput application. This allows unprecedented simplicity and speed for rational design engineering of complex phenotypes in industrial strains.

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Section: Introductionmentioning

confidence: 99%

Strain Engineering by Genome Mass Transfer: Efficient Chromosomal Trait Transfer Method Utilizing Donor Genomic DNA and Recipient Recombineering Hosts

2009

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“…We hypothesized that urinary E. coli F plasmids could influence permissivity to phage infection; we tested this hypothesis by monitoring lysis by coliphages P1vir, Greed, and Lust. P1vir is a laboratory phage mutated to enter only the lytic cycle, and Greed and Lust are urinary phages that lyse E. coli , including some urinary strains ( 8 , 32 , 33 , 42 ). We found F plasmids in most of our urinary E. coli isolates; they often carried genes associated with TA modules, antibiotic resistance, and/or virulence.…”

Section: Introductionmentioning

confidence: 99%

Urinary Plasmids Reduce Permissivity to Coliphage Infection

2023

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“…We hypothesized that urinary E. coli F plasmids could influence permissivity to phage infection; we tested this hypothesis by monitoring lysis by coliphages P1vir, Greed, and Lust. P1vir is a laboratory phage mutated to only enter the lytic cycle; Greed and Lust are urinary phages that lyse E. coli , including some urinary strains 8, 33, 34, 43 . We found F plasmids in most of our urinary E. coli isolates; they often carried genes associated with TA modules, antibiotic resistance, and/or virulence.…”

Section: Introductionmentioning

confidence: 99%

Urinary F plasmids reduce permissivity to coliphage infection

Hernandez

Putonti

Wolfe

2022

Preprint

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The microbial community of the urinary tract (urinary microbiota or urobiota) has been associated with human health. Bacteriophages (phages) and plasmids present in the urinary tract, like in other niches, may shape urinary bacteria dynamics. While urinary E. coli, often associated with urinary tract infection (UTI), and their phages have been catalogued for the urobiome, the dynamics of their interactions have yet to be explored. In this study, we characterized urinary E. coli F plasmids and their ability to decrease permissivity to E. coli phage (coliphage) infection. Putative F plasmids were present in 47 of 67 urinary E. coli isolates, and most of these plasmids carry genes that encode for toxin-antitoxin (TA) modules, antibiotic resistance, and/or virulence. Two urinary E. coli F plasmids, from urinary microbiota (UMB) 0928 and 1284, were conjugated into E. coli K-12 strains; plasmids included genes for antibiotic resistance and virulence. These plasmids, pU0928 and pU1284, decreased permissivity to coliphage infection by the laboratory phage P1vir and the urinary phages Greed and Lust. Furthermore, pU0928 could be maintained in E. coli K-12 for up to ten days in the absence of antibiotic resistance selection; this included maintenance of the antibiotic resistance phenotype and decreased permissivity to phage. Finally, we discuss how F plasmids present in urinary E. coli could play a role in coliphage dynamics and maintenance of antibiotic resistance in urinary E. coli.

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Altered Phage P1 Attachment to Strains of Escherichia Coli Carrying the Plasmid ColV,I-K94

Cited by 4 publications

References 20 publications

Strain Engineering by Genome Mass Transfer: Efficient Chromosomal Trait Transfer Method Utilizing Donor Genomic DNA and Recipient Recombineering Hosts

Strain Engineering by Genome Mass Transfer: Efficient Chromosomal Trait Transfer Method Utilizing Donor Genomic DNA and Recipient Recombineering Hosts

Urinary Plasmids Reduce Permissivity to Coliphage Infection

Urinary F plasmids reduce permissivity to coliphage infection

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