Altered pattern of lipids in plasma and erythrocyte membranes of rheumatoid arthritis patients

Vijayakumar, D.; Suresh, K.; Manoharan, Shanmugam

doi:10.1007/bf02893042

Cited by 13 publications

(6 citation statements)

References 16 publications

(9 reference statements)

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“…16 In other diseases, serum lipid pattern, phospholipid fatty acid composition, and fatty acid unsaturation index, vitamin E content and LPO correlated with the composition and structure of erythrocyte membranes. 17,18 This matches our own fi ndings. However, in this context, it must be mentioned that -different from other diseases -thalassemic hemoglobin plays a deleterious role in the destruction of RBC membranes.…”

Section: Transfusion-dependent Patientssupporting

confidence: 90%

Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Freisleben¹,

Hidayat²,

Freisleben³

et al. 2011

Med J Indones

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Latar belakang: Kami melakukan penelitian selama 10 tahun terakhir untuk mendapatkan gambaran yang komprehensif mengenai kelebihan besi, stres oksidatif, dan kerusakan sel pada pasien talasemia di Jakarta. Metode: Dari sampel darah 15 pasien yang bergantung transfusi (kelompok T), 5 pasien yang belum ditransfusi (kelompok N) dan 10 kontrol (kelompok C) dilakukan analisis plasma dan membran eritrosit yang diisolasi dan strukturnya diteliti dengan spekroskopi electron paramagnetic resonance (EPR) menggunakan doxyl-stearic acid dan maleimido-proxyl spin lable. Data statistik dianalisis dengan ANOVA. Hasil: Terdapat nilai trigliserida lebih tinggi dan nilai kolesterol plasma lebih rendah pada penderita talasemia dibandingkan dengan kontrol. Kadar vitamin E (kelompok C: 21.8 vs T: 6.2 μmol/L) dan reactive thiols (C: 144 vs. T: 61 μmol/L) jauh lebih rendah pada pasien yang bergantung-transfusi. Tanda-tanda stres oksidatif (MDA, C: 1.96 vs. T: 9.2 μmol/L) dan kadar transaminase dalam serum sangat tinggi. Pasien belum ditransfusi memperlihatkan sedikit tanda-tanda terjadinya stres oksidatif, tetapi tidak terdapat tanda-tanda yang berarti adanya kerusakan jaringan atau sel. Parameter membran sel darah merah dari spektroskopi electron paramagnetic resonannce (EPR) menunjukkan hasil yang sangat berbeda-beda untuk setiap kelompok. Pada pasien bergantung-transfusi, struktur membran sel darah merah serta gradien polaritas dan fl uiditas dalam domain lipid menunjukkan rusak; kapasitas mengikat protein tiol di dalam membran lebih rendah dan statis.

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Section: Transfusion-dependent Patientssupporting

confidence: 90%

Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Freisleben¹,

Hidayat²,

Freisleben³

et al. 2011

Med J Indones

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“…Both possibilities suggest a condition of oxidative stress is present in whole blood of RA patients. This is in agreement with reports of increased ROS and biomarkers of oxidative damage in either plasma and/or erythrocytes of RA patients . To our knowledge no reports exist on oxidative stress in the whole blood of RA patients and these studies provide limited information on the true redox status of RA patients .…”

Section: Discussionsupporting

confidence: 92%

Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson's disease patients

2018

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The consequences of oxidative stress and inflammation are implicated in a wide range of diseases, including rheumatoid arthritis and Parkinson's disease. The status of antioxidant capacity in rheumatoid arthritis and Parkinson's disease remains unclear, in part due to common practice of assaying erythrocytes separately to plasma. This method removes any synergistic interactions between plasma and erythrocyte-based antioxidants. The experiments in this report tested antioxidant capacity in whole blood, erythrocytes and plasma by group and disease stage. Medically diagnosed patients were recruited along with appropriate control group participants. Fasting venous blood was assayed using chemiluminescence methods for: time to maximum light emitted, maximum light emitted, and plasma antioxidant capacity in vitamin E analogue units. Here we demonstrate that whole blood exhibits higher antioxidant capacity than either plasma or erythrocytes assayed separately. We report increased oxidative stress in the blood of rheumatoid arthritis patients by group (p = 0.018, p = 0.049). We show increased antioxidant capacity in Parkinson's disease patients by group (p < 0.001). For later stage Parkinson's disease patients, we report reduced oxidative stress (p = 0.025), and increased antioxidant capacity and for erythrocytes (p < 0.001, p = 0.004) and whole blood (p < 0.001, p = 0.003). Early stage Parkinson's disease showed higher antioxidant capacity on only one measure (p = 0.008). Whole blood chemiluminescence is a useful technique for determining redox status in disease and might help clarify the role of oxidative stress in rheumatoid arthritis and Parkinson's disease.

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“…Previous studies have shown an increased level of thiobarbituric acid reactive substances (TBARS) (6.58 ± 0.49, P < 0.001) in RA patients 48 . The present study results show significant reduction in levels of TBARS (a marker of oxidative stress) ( P < 0.01) after drug treatment (Table 2).…”

Section: Discussionsupporting

confidence: 74%

Advanced glycation end‐products inhibition improves endothelial dysfunction in rheumatoid arthritis

Syngle¹,

Vohra

Garg

et al. 2011

Int J of Rheum Dis

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Altered pattern of lipids in plasma and erythrocyte membranes of rheumatoid arthritis patients

Cited by 13 publications

References 16 publications

Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson's disease patients

Advanced glycation end‐products inhibition improves endothelial dysfunction in rheumatoid arthritis

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