Altered nuclear pore diameters in G1-arrested cells of the yeast Saccharomyces cerevisiae

Willison, J. H. M.; Johnston, Gerald C.

doi:10.1128/jb.136.1.318-323.1978

Cited by 14 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Complementary data gained from different fractions showed that the yeast NPC is also significantly smaller than vertebrate NPCs. This has been reported by others (Moor and Miihlethaler, 1963;Severs et al, 1976;Willison and Johnston, 1978). However the overall morphology and the diameters of the central pore and plug within appear to be conserved.…”

Section: Discussionsupporting

confidence: 80%

“…Thus it is not clear whether the isolated NPCs retained such rings, nor if they are present in yeast NPCs at all. Surprisingly, the measurements obtained from the NPCs within the context of the yeast nuclei were very similar to those obtained from the isolated NPCs (Table IO, though they are in full agreement with the dimensions of NPCs as seen in preparations of yeast cells (Moor and Miihlethaler, 1963;Severs et al, 1976;Willison and Johnston, 1978;Byers, 1981).…”

Section: Electron Microscopy Of the Npc Fractionssupporting

confidence: 74%

See 1 more Smart Citation

Isolation of the yeast nuclear pore complex.

Rout

Blobel

1993

The Journal of Cell Biology

262

284

View full text Add to dashboard Cite

Abstract. Nuclear pore complexes (NPCs) have been isolated from the yeast Saccharomyces. Negative stain electron microscopy of the isolated NPCs and subsequent image reconstruction revealed the octagonal symmetry and many of the ultrastructural features characteristic of vertebrate NPCs. The overall dimensions of the yeast NPC, both in its isolated form as well as in situ, are smaller than its vertebrate counterpart. However, the diameter of the central structures are similar. The isolated yeast NPC has a sedimentation coefficient of '-,310 S and an Mr of ',,66 MD. It retains all but one of the eight known NPC proteins. In addition it contains as many as 80 uncharacterized proteins that are candidate NPC proteins.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Electron Microscopy Of the Npc Fractionssupporting

confidence: 74%

Isolation of the yeast nuclear pore complex.

Rout

Blobel

1993

The Journal of Cell Biology

262

284

View full text Add to dashboard Cite

show abstract

“…Pore complexes in actively growing yeast cells appear to have a normal diameter (,x,100 nm), and are present at a frequency ("~11 pores/~m 2) identical to that in many other cell types (14,18). Interestingly, slow growth or growth arrest results in fewer pores per nucleus or an increase in pore diameter (14,28). Pore synthesis during interphase of the yeast cell cycle may be comparable to that in higher eucaryotes (14,19,22).…”

mentioning

confidence: 94%

Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins.

Aris

Blobel

1989

The Journal of Cell Biology

111

View full text Add to dashboard Cite

Abstract. We have used a monoclonal antibody raised against rat liver nuclear proteins to study two crossreactive proteins in the yeast nucleus. In rat liver, this monoclonal antibody, mAb 414, binds to nuclear pore complex proteins, including one of molecular weight 62,000 (Davis, L. I., and G. Blobel. 1987. Proc. Natl. Acad. Sci. USA. 84:7552-7556). In yeast, mAb 414 cross reacts by immunoblotting with two proteins that have apparent molecular weights of 110,000 and 95,000, and are termed p110 and p95, respectively. Examination of subcellular fractions by immunoblotting shows that both pll0 and p95 are located exclusively in the nuclear fraction. The mAb 414 immunoprecipitates several proteins from a crude yeast cell extract, including pll0, p95, and a ,x,55-kD protein. Immunoprecipitation from subcellular fractions yields only p110 and p95 from purified nuclei, whereas the ~55-kD protein is immunoprecipitated from the soluble fraction. Digestion of purified nuclei with DNase to produce nuclear envelopes releases some of pll0, but the majority of pll0 is solubilized only after treatment of envelopes with 1 M NaCI. Immunofluorescence localization using yeast cells and isolated nuclei shows a punctate and patchy staining pattern of the nucleus. Confocal laser scanning immunofluorescence microscopy resolves the punctate and patchy staining pattern better and shows regions of fluorescence at the nuclear envelope. Postembedding immunogold electron microscopy using purified nuclei and mAb 414 shows colloidal gold decoration of the yeast nuclear envelope, but resolves pore complexes too poorly to achieve further ultrastructural localization. Immunogold labeling of nuclei followed by embedding suggests decoration of pore complexes. Thus, pll0 and/or p95 are localized to the nuclear envelope in yeast, and may be components of the nuclear pore complex.

show abstract

“…The model shows that despite the initial size differences the generation times of the mother and daughter cells are equal. small yeast cells was reported recently by Willison and Johnston (30). They showed that the nuclear pores of small Gl-arrested cells were larger in diameter than those of large Gl-arrested cells.…”

Section: Resultsmentioning

confidence: 62%

Differential growth rates of Candida utilis mother and daughter cells under phased cultivation

Thomas¹,

Dawson²,

Gamborg³

1980

J Bacteriol

View full text Add to dashboard Cite

The yeast Candida utilis was continuously synchronized by the phased method of cultivation with the nitrogen source as the growth-limiting nutrient. The doubling time (phasing period) of celLs was 6 h. Both cell number and deoxyribonucleic acid synthesis showed a characteristic stepwise increase during the phased growth. The time of bud emergence coincided with the time of initiation of deoxyribonucleic acid synthesis. Size distribution studies combined with microscopic analysis showed that the cells expanded only during the unbudded phase of growth. Usually the cells stopped increasing in size about 30 min before bud emergence, and the arrest of the increase in cell volume coincided with the exhaustion of nitrogen from the medium. There was no net change in the volume of cells during the bud expansion phase of growth, suggesting that as the bud expanded, the volume of the mother portion of the cell decreased. After division the cells expanded slightly. The postdivision expansion of cells, unlike the growth before bud initiation, occurred in the absence of the growth-limiting nutrient. The newly formed daughter cells were smaller than the mother cells and expanded at a faster rate, so that both types of cells reached maximum size at the same time. Possible reasons for the different rates of expansion of mother and daughter cells are discussed. Mitchison (18) suggested that the cell cycle can be considered as two loosely coupled and temporally overlapping sequences ofevents. One is referred to as the DNA division cycle, and the other is referred to as the growth cycle. Cycle markers that can be identified with the DNA division cycle are the start and the finish of the S period, stages of mitosis, and cell division. Most of the macromolecular syntheses, including the production of step and peak enzymes and cell envelope synthesis, are considered to be part of the growth cycle. Under normal conditions of growth these cycles are coordinated. This is borne out by the fact that cells of a particular type display a characteristic and rather narrow range of sizes (7). Cell division without precisely controlled coordination between growth and DNA division cycles would result in the production of excessively large or infinitesimally small cells. However, the mechanism of this coordination is poorly understood. One suggestion is that attainment of a critical cell size might be a prerequisite for the continuation of the cellular events that lead to cell division (8). The existence of such "size control" over cell division has been verified in several microorganisms (4,7,8,10,32). t National Research Council publication no. 17960.The progression through the cell cycle of two newborn cells of equal size under the same environmental conditions would be expected to be at the same rate, so that the cells might attain the critical size at the same time. Most likely these cells would have the same generation time as well. However, if the newborn cells were not the same size, it is quite likely that the smaller cell would take a lo...

show abstract

Altered nuclear pore diameters in G1-arrested cells of the yeast Saccharomyces cerevisiae

Cited by 14 publications

References 13 publications

Isolation of the yeast nuclear pore complex.

Isolation of the yeast nuclear pore complex.

Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins.

Differential growth rates of Candida utilis mother and daughter cells under phased cultivation

Contact Info

Product

Resources

About