Altered mRNA Expression due to Acute Mesenteric Ischaemia in a Porcine Model

Block, Tomas; Isaksson, Helena S.; Acosta, Stefan; Björck, Martin; Brodin, David; Nilsson, Torbjörn

doi:10.1016/j.ejvs.2010.09.012

Cited by 9 publications

(10 citation statements)

References 35 publications

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“…ischemia and inflammation in various organs (21)(22)(23). Also, we do not know whether some of our patients had circulating tumor cells in Table II.…”

Section: Discussionmentioning

confidence: 97%

Whole blood RNA expression profiles in ovarian cancer patients with or without residual tumors after primary cytoreductive surgery

2012

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Abstract. Significant improvements in the treatment results of ovarian cancer have been achieved during the last decades, but further improvements require additional methods identifying signs of the disease and its biological behavior, preferably by a simple blood test. We hypothesized that peripheral blood leukocytes may express genes that carry such clinical information. Therefore, we studied the relative gene expressions of 168 cancer-and metastasis-specific genes in blood samples from ovarian cancer patients with different prognoses after primary cytoreductive surgery. Total RNA was extracted from whole blood and the relative gene expression profile of 168 genes were analyzed using real-time qPCR assays. Two groups of patients were analyzed; one group with residual tumor mass after primary surgery, and one group where the tumor was macroscopically radically resected, resulting in no visible tumor mass left behind. The group with the remaining tumor mass after surgery showed significantly different gene expression profiles compared to the group with no remaining tumor mass. Differences were noted for the metastasis associated 1 family, member 2 gene (MTA2), the TNF, α-catenin, interleukin 1β, the KiSS-1 metastasis suppressor and the matrix metallo proteinase 10 genes. All genes were downregulated with a fold-change between 1.15 to 1.57; there were no upregulated genes. Thus, a signature of genes involved in metastasis, invasion and inflammation was found to be significantly downregulated in native unstimulated blood leukocytes from ovarian cancer patients with a poor prognosis. Preoperatively it may serve as a guide to the biology of the tumor and postoperatively in the optimization of adjuvant treatment of ovarian cancer patients.

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“…ischemia and inflammation in various organs (21)(22)(23). Also, we do not know whether some of our patients had circulating tumor cells in Table II.…”

Section: Discussionmentioning

confidence: 97%

Whole blood RNA expression profiles in ovarian cancer patients with or without residual tumors after primary cytoreductive surgery

2012

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“…Of other large animals used in biomedical research, dogs have been used extensively, particularly for the study of ischemia/ reperfusion injury. However, with increasing social pressures to limit use of dogs as experimental animals and the high mortality rate associated with some disease models, the use of dogs is declining (18, 23). However, dogs are particularly well suited and are increasingly utilized for the study of spontaneous naturally occurring diseases that also affect humans, such as cancer.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, pharmaceutical bioavailability and nutrient digestibility in pigs closely resemble that of humans (19, 25, 43). These characteristics have led to the use of pigs for the development of pig models of a number of gastrointestinal diseases including necrotizing enterocolitis (16, 44–48), acute mesenteric ischemia (18, 49–52), short bowel syndrome (43, 53–57), AIDS-associated Cryptosporidium infection (17, 58, 59), stress-induced intestinal dysfunction (60–63), cystic fibrosis (64, 65) and familial adenomatous polyposis (14). This review will highlight strengths and limitations of pig models of intestinal ischemia/reperfusion injury, stress-induced intestinal dysfunction and short bowel syndrome.…”

Section: Introductionmentioning

confidence: 99%

Porcine models of digestive disease: the future of large animal translational research

Gonzalez

Moeser

Blikslager

2015

Translational Research

174

167

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There is increasing interest in non-rodent translational models for the study of human disease. The pig, in particular, serves as a useful animal model for the study of pathophysiological conditions relevant to the human intestine. This review assesses currently used porcine models of gastrointestinal physiology and disease and provides a rationale for the use of these models for future translational studies. The pig has proven its utility for the study of fundamental disease conditions such as ischemia/ reperfusion injury, stress-induced intestinal dysfunction, and short bowel syndrome. Pigs have also shown great promise for the study of intestinal barrier function, surgical tissue manipulation and intervention, as well as biomaterial implantation and tissue transplantation. Advantages of pig models highlighted by these studies include the physiological similarity to human intestine as well as to mechanisms of human disease. Emerging future directions for porcine models of human disease include the fields of transgenics and stem cell biology, with exciting implications for regenerative medicine.

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“…In a pig model with induced proximal arterial occlusion of the superior mesenteric artery, Block et al identified a panel of up-and down-regulated mRNA (157 and 57 transcripts, respectively). Up-regulated mRNA included monocyte chemoattractant protein 1 (MCP1) and acyl CoA synthetase long-chain family member 4 [77]. In a preclinical study in swine, a microRNA signature was identified in an i3 model related to hypothermic circulatory arrest associated with the gut barrier dysfunction.…”

Section: Towards Promising Novel Approaches For Finding Biomarkersmentioning

confidence: 99%

Diagnosis biomarkers in acute intestinal ischemic injury: so close, yet so far

Peoc’h

Nuzzo

Guedj

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Acute intestinal ischemic injury (i3) is a lifethreatening condition with disastrous prognosis, which is currently difficult to diagnose at the early stages of the disease; a rapid diagnosis is mandatory to avoid irreversible ischemia, extensive bowel resection, sepsis and death. The overlapping protein expression of liver and gut related to the complex physiopathology of the disease, the heterogeneity of the disease and its relative rarity could explain the lack of a useful early biochemical marker of i3. Apart from non-specific biological markers of thrombosis, hypoxia inflammation, and infection, several more specific biomarkers in relation with the gut barrier dysfunction, the villi injury and the enterocyte mass have been used in the diagnosis of acute i3. It includes particularly D-lactate, intestinal fatty acid-binding protein (FABP) and citrulline. Herein, we will discuss leading publications concerning these historical markers that point out the main limitations reagrding their use in routine clinical practice. We will also introduce the first and limited results arising from omic studies, underlying the remaining effort that needs to be done in the field of acute i3 biological diagnosis, which remains a challenge.

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Altered mRNA Expression due to Acute Mesenteric Ischaemia in a Porcine Model

Cited by 9 publications

References 35 publications

Whole blood RNA expression profiles in ovarian cancer patients with or without residual tumors after primary cytoreductive surgery

Whole blood RNA expression profiles in ovarian cancer patients with or without residual tumors after primary cytoreductive surgery

Porcine models of digestive disease: the future of large animal translational research

Diagnosis biomarkers in acute intestinal ischemic injury: so close, yet so far

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