Altered microRNA profiles in cerebrospinal fluid exosome in Parkinson disease and Alzheimer disease

Gui, Yaxing; Liu, Hai; Zhang, Li Shan; Lv, Wen; Hu, Xing

doi:10.18632/oncotarget.6158

Cited by 462 publications

(419 citation statements)

References 42 publications

(46 reference statements)

Supporting

Mentioning

404

Contrasting

Unclassified

Order By: Relevance

“…Our findings were consistent with the results of previous studies showing that STAT3 was negatively regulated by miR-29c-3p in brain tissues (Lei et al 2015). Other studies demonstrated that miR19b3p levels were significantly decreased in the cerebrospinal fluid of AD patients (Gui et al 2015). However, few reports have described target genes of miR19b-3p.…”

Section: Discussionmentioning

confidence: 96%

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are short noncoding RNA that participate in posttranscriptional gene regulation. However, little is understood about the roles of miRNAs in Alzheimer's disease (AD). In this study, we used next-generation sequencing on RNA extracted from the serum samples of 20 AD patients and 20 controls, yielding a total of 72 miRNAs with significantly changed expression levels. Among these candidates, we selected 9 miRNAs with most significant alteration in disease, and validated their expression levels using RT-qPCR analysis on serum samples from 45 AD patients and 40 control subjects. Thus, the serum levels of miR-146a-5p, 106b-3p, 195-5p, 20b-5p, and 497-5p were significantly higher, while those of miR-125b-3p, 29c-3p, 93-5p and 19b-3p were significantly lower in AD patients, compared with control subjects. Two miRNAs, miR-29c-3p and miR-19b-3p, were selected because both RNA deep-sequencing and q-PCR methods indicated lower serum levels of these miRNAs in AD patients. Computational analysis predicted that 3′-untranslated region of signal transduction and activator of transcription 3 (STAT3) mRNA is targeted both by miR-29c-3p and miR-19b-3p. Using SH-SY5Y human neuroblastoma cells, we showed that transfection with miR-29c-3p or miR-19b-3p inhibitor significantly increased STAT3 phosphorylation. Furthermore, Water maze test, which assesses the learning and memory deficits in rodents, showed that escape latency was significantly shorter in AD rats with overexpression of miR-29c-3p or miR-19b-3p than in control AD rats. These results suggest that miR-29c-3p or miR-19b-3p may contribute to the cognitive function. In conclusion, the serum levels of miR-29c-3p and miR-19b-3p are helpful biomarkers for AD.

show abstract

Section: Discussionmentioning

confidence: 96%

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

show abstract

“…Exosomes in the embryonic CSF have also been shown to modulate IGF signalling and promote Neural Stem Cell proliferation (NSC) (Feliciano et al, 2014). Exosomes have been proposed as promising non‐invasive diagnostic tools for cancer (Skog et al, 2008), kidney diseases (Spanu et al, 2014) and neurodegeneration (Liu et al, 2014; Gui et al, 2015) but have not been explored in neonatal development and GMH‐IVH/PHH.…”

Section: Introductionmentioning

confidence: 99%

Exosomes populate the cerebrospinal fluid of preterm infants with post‐haemorrhagic hydrocephalus

Spaull

McPherson

Gialeli

et al. 2019

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Background Preterm infants are at risk of germinal matrix haemorrhage‐intraventricular haemorrhage (GMH‐IVH) which leads to post‐haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate‐severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long‐term outcome after GMH‐IVH. In recent years, endosome‐derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro‐RNAs. Methods This study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro‐RNA analysis. Results NTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal‐sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro‐RNAs miR‐9, miR‐17, miR‐26a, miR‐124 and miR‐1911 were detected within the exosome‐enriched fraction and profiled over time. Conclusion This is the first reported characterisation of exosomes from the CSF of preterm infants with post‐haemorrhagic hydrocephalus.

show abstract

“…miRNAs impact on α-synuclein expression raises the hypothesis that dysregulated miRNAs in PD patients are responsible for α-synuclein upregulation and/or accumulation. Supporting this idea, several studies have demonstrated that PD patients exhibited dysregulated miRNAs in brain (Kim et al, 2007; Cardo et al, 2013; Miñones-Moyano et al, 2013; Briggs et al, 2015; Hoss et al, 2016), blood (Margis et al, 2011; Martins et al, 2011; Khoo et al, 2012; Botta-Orfila et al, 2014; Burgos et al, 2014), cerebrospinal fluid (CSF; Burgos et al, 2014; Gui et al, 2015; Hossein-Nezhad et al, 2016) and medulla (Liao et al, 2013). …”

Section: Discussionmentioning

confidence: 99%

Role of microRNAs in the Regulation of α-Synuclein Expression: A Systematic Review

Recasens

Perier

Sue

2016

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Growing evidence suggests that increased levels of α-synuclein might contribute to the pathogenesis of Parkinson’s disease (PD) and therefore, it is crucial to understand the mechanisms underlying α-synuclein expression. Recently, microRNAs (miRNAs) have emerged as key regulators of gene expression involved in several diseases such as PD and other neurodegenerative disorders. A systematic literature search was performed here to identify microRNAs that directly or indirectly impact in α-synuclein expression/accumulation and describe its mechanism of action. A total of 27 studies were incorporated in the review article showing evidences that six microRNAs directly bind and regulate α-synuclein expression while several miRNAs impact on α-synuclein expression indirectly by targeting other genes. In turn, α-synuclein overexpression also impacts miRNAs expression, indicating the complex network between miRNAs and α-synuclein. From the current knowledge on the central role of α-synuclein in PD pathogenesis/progression, miRNAs are likely to play a crucial role at different stages of PD and might potentially be considered as new PD therapeutic approaches.

show abstract

Altered microRNA profiles in cerebrospinal fluid exosome in Parkinson disease and Alzheimer disease

Cited by 462 publications

References 42 publications

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

Exosomes populate the cerebrospinal fluid of preterm infants with post‐haemorrhagic hydrocephalus

Role of microRNAs in the Regulation of α-Synuclein Expression: A Systematic Review

Contact Info

Product

Resources

About