Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome

Li, Yahan; Hagen, Darren E.; Ji, Tieming; Bakhtiarizadeh, Mohammad Reza; Frederic, Whitney M; Traxler, Emily M; Kalish, Jennifer M.

doi:10.1080/15592294.2019.1615357

Cited by 37 publications

(46 citation statements)

References 91 publications

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“…MicroR-296-3p was previously reported to be expressed in ovaries in mice 60 and to repress cell plasticity in different tumour lines 61 , promoting apoptosis in liver 62 and in mammalian pancreatic α cells 63 . Recently, altered expression of bta-miR-296-3p was detected in muscle, kidney, and liver, in bovine foetuses with large offspring syndrome (LOS) 64 . In addition, miR-296 was also observed to be epigenetically regulated as a part of the imprinted Gnas/GNAS clusters 65 .…”

Section: Discussionmentioning

confidence: 99%

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Capra

Lazzari

Russo

et al. 2020

Sci Rep

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Season clearly influences oocyte competence in buffalo (Bubalus bubalis); however, changes in the oocyte molecular status in relation to season are poorly understood. This study characterizes the microRNA (miRNA) and transcriptomic profiles of oocytes (OOs) and corresponding follicular cells (FCs) from buffalo ovaries collected in the breeding (BS) and non-breeding (NBS) seasons. In the BS, cleavage and blastocyst rates are significantly higher compared to NBS. Thirteen miRNAs and two mRNAs showed differential expression (DE) in FCs between BS and NBS. DE-miRNAs target gene analysis uncovered pathways associated with transforming growth factor β (TGFβ) and circadian clock photoperiod. Oocytes cluster in function of season for their miRNA content, showing 13 DE-miRNAs between BS and NBS. Between the two seasons, 22 differentially expressed genes were also observed. Gene Ontology (GO) analysis of miRNA target genes and differentially expressed genes (DEGs) in OOs highlights pathways related to triglyceride and sterol biosynthesis and storage. Co-expression analysis of miRNAs and mRNAs revealed a positive correlation between miR-296-3p and genes related to metabolism and hormone regulation. In conclusion, season significantly affects female fertility in buffalo and impacts on oocyte transcriptomic of genes related to folliculogenesis and acquisition of oocyte competence. Water buffalo (Bubalus bubalis) is an important livestock resource for both developing and developed countries. The major factor affecting buffalo farming profitability is reproductive seasonality, resulting in cycles of calving and milk production. Buffalo is a short-day breeder, with increased fertility in response to decreasing day length 1,2. This photoperiod-dependent seasonality pattern is more pronounced as distance from the equator, together with variations in the light/dark ratio, increases. In Italy, in order to satisfy market demand, out of breeding mating strategy (OBMS), consisting in interrupting sexual promiscuity or the use of artificial insemination (AI) during the breeding season (BS), is commonly utilized 2. The OBMS improves the distribution of calving throughout the year, but it reduces fertility 3. Longer post-partum anoestrus periods as well as higher incidence of embryonic mortality are observed in months with increasing daylight length and particularly in midwinter , which coincides with the transition to seasonal anoestrus at Italian latitudes 1,4. The embryonic mortality is due to inadequate luteal growth and function, resulting in reduced progesterone secretion 5. This has a negative impact on embryo growth, associated with alterations in transcriptomic and proteomic profiles of the embryos and chorioamnios/caruncles 6,7 , which ultimately impair embryo attachment to the uterine endometrium.

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Section: Discussionmentioning

confidence: 99%

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Capra

Lazzari

Russo

et al. 2020

Sci Rep

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“…Indeed, in cattle and sheep it has been noted a higher incidence of embryo loss, higher pregnancy length, large body size and birth weight in ART-derived gestations [25]. In addition, the large offspring syndrome described in cattle is an imprinting disorder with similar features to Beckwith-Wiedemann Syndrome in humans, such as macrosomia, macroglossia or visceromegaly [26,27]. Other anomalies observed in IVP-derived cattle due to an overgrowth in late gestation are a higher incidence of dystocia, hydrallantois, and neonatal mortality [23,24].…”

Section: Discussionmentioning

confidence: 99%

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

París-Oller

Navarro-Serna

Soriano‐Úbeda

et al. 2020

Preprint

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Background: In vitro embryo production (IVP) and embryo transfer (ET) are two very common assisted reproductive technologies (ART) in human and cattle. However, in pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to the low efficiency of IVP plus the difficulty of performing ET in the long and contorted uterus of the sow. In addition, the potential impact of these two ART on the health of the offspring is unknown. We investigated here if the use of an improved IVP system, with natural reproductive fluids (RF) as supplements to the culture media, combined with a minimal invasive surgery to perform ET, affects the output of the own IVP system as well as the reproductive performance of the mother and placental molecular traits.Results: The blastocyst rates obtained by both in vitro systems, conventional (C-IVP) and improved (RF-IVP), were similar. Pregnancy and farrowing rates were also similar. However, when compared to in vivo control (artificial insemination, AI), litter sizes of both IVP groups were lower, while placental efficiency was higher in AI than in RF-IVP. Gene expression studies revealed aberrant expression levels for PEG3 and LUM in placental tissue for C-IVP group when compared to AI, but not for RF-IVP group. Conclusions: The use of reproductive fluids as additives for the culture media in pig IVP does not improve reproductive performance of recipient mothers but can mitigate the impact of artificial procedures in the offspring.

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“…In our attempt to investigate if the birth weight was affected by sex, we found that only females showed differences when the data were analysed separately, being the weight at birth higher in ET than in AI, as others reported [11], but such differences disappeared on days 3 and 15. This fact, however, should be kept in mind for future studies on the long-term phenotypes of ET animals because, as it has been shown in different studies, increased birth weight is one of the most common ndings in ARTderived calves and it has been related to the large offspring syndrome [24,25] as well as to the Beckwith-Wiedemann syndrome in humans [26], although it has not been described in pigs until now. Another reason not to obviate this nding is that the differences were observed only in females, which are the animals kept in the farm as future mothers for next generations.…”

Section: Discussionmentioning

confidence: 99%

Impact of Embryo Transfer on the phenotype of the offspring in pigs

París-Oller¹,

Matás²,

Romar³

et al. 2020

Preprint

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BackgroundThe increased use of assisted reproductive technologies led to a higher risk of specific alterations in the offspring, as already described in human and other mammalian species. Nonetheless, it is yet to be totally understood how and which specific techniques are inducing these changes. The use of animal models may represent an advantage to find out answers to these questions due to the minimization of confounding factors. The pig is not only a great human reproductive model, but it is also of interest in the livestock industry to develop further technologies that may enhance meat production. This study was designed to decipher the impact of one of these technologies, namely embryo transfer, on mother´s reproductive performance and on the phenotype of the offspring in pigs.ResultsEmbryo transfer of in vivo derived embryos (ET) showed similar results in terms of pregnancy length, litter size and total litter weight per sow than sows artificially inseminated (AI). Bodyweight at birth was higher for ET-females than AI-females, but this difference was no longer observed at day 15 of postnatal life. Some of the major findings after haematological analyses were that concentration of red blood cells, haemoglobin and haematocrit in female-ET piglets at day 3 showed higher values than AI-females. Additionally, higher concentration of white blood cells was found at day 3 in both ET-derived piglets (males and females) in comparison to AI-piglets. Biochemical analysis showed a higher level of albumin at day 3 for ET-derived male piglets than AI but ET-females showed lower levels of bilirubin than AI in the same day. Despite the statistical differences between groups, all values were within the normal ranges, so that we cannot give them any clinical significance.ConclusionsPiglets derived from in vivo embryo transfer seem to be phenotypically similar, which provides evidence that the embryo transfer procedure is a safe technique, without relevant health implications to the offspring. Additionally, these findings may be of interest to the pig industry regarding the reference values provided for haematological and biochemical parameters in this species.

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Altered microRNA expression profiles in large offspring syndrome and Beckwith-Wiedemann syndrome

Cited by 37 publications

References 91 publications

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

Impact of Embryo Transfer on the phenotype of the offspring in pigs

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