Altered metabolomic profile of dual-species biofilm: Interactions between Proteus mirabilis and Candida albicans

Kart, Didem; Ciftci, Samiye Yabanoglu; Nemutlu, Emirhan

doi:10.1016/j.micres.2019.126346

Cited by 20 publications

(20 citation statements)

References 57 publications

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“…A possible explanation of this situation might be that the peptide counteracts the formation of biofilm in polymicrobial system maintaining an active oxidative metabolism. This interpretation is consistent with previous studies which stresses the role of amino acids upregulation in the process of biofilm formation and reveal that genes involved in amino acid biosynthesis are upregulated in C. albicans biofilm compared to planktonic cells [43][44][45].…”

Section: Discussionsupporting

confidence: 93%

“…Apart from that, lower levels of serine in dual bacterial/fungal biofilms, with respect to fungal biofilms, are common. For instance, in a previous work performed to elucidate the metabolomic differences between single and polymicrobial biofilm cultures of P. mirabilis and C. albicans [43], a decrease of about 125 times in the serine level of polymicrobial biofilm cultures, with respect to single species C. albicans biofilm, was observed. Finally, it appears that coexistence of K. pneumoniae and C. albicans increases the uptake of serine in the untreated dual species biofilm with respect to the WMR-K treated biofilm.…”

Section: Discussionmentioning

confidence: 96%

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Impact of the Peptide WMR-K on Dual-Species Biofilm Candida albicans/Klebsiella pneumoniae and on the Untargeted Metabolomic Profile

et al. 2021

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In recent years, the scientific community has focused on the development of new antibiotics to address the difficulties linked to biofilm-forming microorganisms and drug-resistant infections. In this respect, synthetic antimicrobial peptides (AMPs) are particularly regarded for their therapeutic potential against a broad spectrum of pathogens. In this work, the antimicrobial and antibiofilm activities of the peptide WMR-K towards single and dual species cultures of Candida albicans and Klebsiella pneumoniae were investigated. We found minimum inhibitory concentration (MIC) values for WMR-K of 10 µM for K. pneumoniae and of 200 µM for C. albicans. Furthermore, sub-MIC concentrations of peptide showed an in vitro inhibition of biofilm formation of mono and polymicrobial systems and also a good biofilm eradication even if higher concentrations of it are needed. In order to provide additional evidence for the effect of the examined peptide, a study of changes in extracellular metabolites excreted and/or uptaken from the culture medium (metabolomic footprinting) in the poly-microbial association of C. albicans and K. pneumoniae in presence and absence of WMR-K was performed. Comparing to the untreated dual species biofilm culture, the metabolomic profile of the WMR-K treated culture appears significantly altered. The differentially expressed compounds are mainly related to the primary metabolic pathways, including amino acids, trehalose, pyruvic acid, glycerol and vitamin B6.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 96%

Impact of the Peptide WMR-K on Dual-Species Biofilm Candida albicans/Klebsiella pneumoniae and on the Untargeted Metabolomic Profile

et al. 2021

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“…However, Back-Brito et al (2011) have found considerably higher numbers of enteric bacteria in the oral cavities of HIV-positive patients, and Enterobacter cloacae were the most frequently isolated species (Table 1, the search strategy is in the supplementary file, Table S1). Interestingly, it was found that the presence of Candida albicans in the oral cavity can increase the growth and the swarming activity of Proteus mirabilis (Kart et al 2020).…”

Section: Enterobacteriaceaementioning

confidence: 99%

Presence of non-oral bacteria in the oral cavity

Zaatout

2021

Arch Microbiol

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A homeostatic balance exists between the resident microbiota in the oral cavity and the host. Perturbations of the oral microbiota under particular conditions can contribute to the growth of non-oral pathogens that are hard to kill because of their higher resistance to antimicrobials, raising the probability of treatment failure and reinfection. The presence of these bacteria in the oral cavity has been proven to be associated with several oral diseases such as periodontitis, caries, and gingivitis, and systemic diseases of importance in clinical medicine such as cystic fibrosis, HIV, and rheumatoid arthritis. However, it is still controversial whether these species are merely transient members or unique to the oral cavity. Mutualistic and antagonistic interactions between the oral microbiota and non-oral pathogens can also occur, though the mechanisms used by these bacteria are not clear. Therefore, this review presents an overview of the current knowledge about the presence of non-oral bacteria in the oral cavity, their relationship with systemic and oral diseases, and their interactions with oral bacteria. Supplementary Information The online version contains supplementary material available at 10.1007/s00203-021-02300-y.

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“…Metabolomic analyses of Staphylococcus aureus and C. albicans mixed biofilms showed that the symbiotic coexistence of the two species is signified by the high abundance of sedoheptulose-7-phosphate, an intermediate of the pentose phosphate pathway [36]. By contrast, the antagonistic effect of Proteus mirabilis on C. albicans growth resulted in slower metabolism and energy consumption by the fungus within the mixed biofilm [37]. Thus, metabolomics has an enormous potential to define interspecies interactions, which might be difficult or even impossible to achieve with other approaches.…”

Section: Biofilm Formationmentioning

confidence: 99%

“…By contrast, the antagonistic effect of Proteus mirabilis on C . albicans growth resulted in slower metabolism and energy consumption by the fungus within the mixed biofilm [ 37 ]. Thus, metabolomics has an enormous potential to define interspecies interactions, which might be difficult or even impossible to achieve with other approaches.…”

Section: What Have We Learned From Metabolomics Of Human Pathogenic Fmentioning

confidence: 99%

Catch the wave: Metabolomic analyses in human pathogenic fungi

2020

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Altered metabolomic profile of dual-species biofilm: Interactions between Proteus mirabilis and Candida albicans

Cited by 20 publications

References 57 publications

Impact of the Peptide WMR-K on Dual-Species Biofilm Candida albicans/Klebsiella pneumoniae and on the Untargeted Metabolomic Profile

Impact of the Peptide WMR-K on Dual-Species Biofilm Candida albicans/Klebsiella pneumoniae and on the Untargeted Metabolomic Profile

Presence of non-oral bacteria in the oral cavity

Catch the wave: Metabolomic analyses in human pathogenic fungi

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