Altered metabolism of chloroplastic NAD kinase-overexpressing Arabidopsis in response to magnesium sulfate supplementation

Kawai‐Yamada, Maki; Miyagi, Atsuko; Sato, Yuki; Hosoi, Yuki; Hashida, Shin‐nosuke; Ishikawa, Toshiki; Yamaguchi, Masatoshi

doi:10.1080/15592324.2020.1844509

Cited by 2 publications

(1 citation statement)

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“…Two principal components, PC1 (31%) and PC2 (17%), together accounted for 48% of variance in the metabolite dataset ( Figure 2a ). The results of the PCA showed that the metabolic patterns of the WT plants and nadk2 mutants were quite different in the non-senescent leaves at day 0, as reported previously 10 , 21 . In the non-senescent leaves, different metabolic profiles were observed at day 1, 3, and 7.…”

Section: Resultssupporting

confidence: 82%

Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants

Chaomurilege

Miyagi

Ishikawa

et al. 2023

Plant Signaling & Behavior

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Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP + synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence.

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Section: Resultssupporting

confidence: 82%