Altered metabolism and function of rat heart following chronic low level cadmium/lead feeding

Kopp, SJ; Glonek, Thomas; Erlanger, M; Ef, Perry; Bárány, Michael; Hm, Perry

doi:10.1016/0022-2828(80)90125-x

Cited by 27 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, in the rat heart the relative phosphorylcholine level is about 1%; in the guinea pig brain the level is 3%. The unknown resonances at 106 in the brain spectrum are also observed in liver (Kopp et al, 1980) and lens (Greiner et al, 1981) spectra, although they have not been detected in white muscle spectra (Burt et al, 1976~).…”

Section: Discussionmentioning

confidence: 99%

P‐31 Nuclear Magnetic Resonance Analysis of Brain: The Perchloric Acid Extract Spectrum

Glonek¹,

Kopp²,

Kot

et al. 1982

Journal of Neurochemistry

164

View full text Add to dashboard Cite

Perchloric acid (PCA) extracts were prepared from liquid‐N2‐frozen guinea pig brains and their organophosphate profiles examined by P‐31 nuclear magnetic resonance (NMR) spectroscopy. Thirty‐two phosphorus‐containing brain metabolites were characterized and quantitated. A distinctive feature of brain tissue metabolism relative to that of other tissues probed by P‐31 NMR is its pronounced ribose 5‐phosphate content. Comparison of brain metabolite levels following control or sublethal cyanide treatment (4 mg/kg) revealed specific cyanide‐induced changes in brain metabolism. Brains from cyanidetreated animals were characterized by a reduced phosphocreatine content and elevated α‐glycerolphosphate and inorganic orthophosphate contents relative to control. P‐31 NMR spectra of brain PCA extracts at pH 7.2 were also obtained under conditions that approximate those used for in vivo and intact tissue in vitro P‐31 spectroscopic analyses. The spectra reveal nine separate resonance bands corresponding to: sugar phosphates, principally ribose 5‐phosphate (3.7δ); inorganic orthophosphate (2.2δ); glycerol 3‐phosphorylethanolamine (0.3δ); glycerol 3‐phosphorylcholine (−0.1δ); phosphocreatine (−3.2δ); adenosine tri‐(β‐ATP) and di‐(β‐ADP) phosphate ionized end‐groups (−6.2δ); α‐ATP, α‐ADP, and nicotinamide adenine dinucleotides esterified end‐groups (−11.1δ); uridine diphosphohexose, hexose esterified end‐groups (−13.0δ); and β‐ATP ionized middle group (−21.6δ). Knowledge of the phosphatic molecules that contribute resonances to the brain P‐31 NMR spectrum as well as understanding their magnetic resonance properties is essential for the interpretation of in vivo brain spectroscopic data as well as brain extract data, since these same compounds contribute to the intact brain P‐31 spectrum.

show abstract

Section: Discussionmentioning

confidence: 99%

P‐31 Nuclear Magnetic Resonance Analysis of Brain: The Perchloric Acid Extract Spectrum

Glonek¹,

Kopp²,

Kot

et al. 1982

Journal of Neurochemistry

164

View full text Add to dashboard Cite

show abstract

“…Ouabain is the first or ganic molecule wc have tested which has a demonstrated effect on the 18-9 6 resonance region [4,7,8]. Usually the concentrations of the metabolites giving rise to these signals in various mammalian tissues are constant from sample to sample unless the source ani mal has experienced a severe pahtology [9,10]. Since the chemical structures of these phosphatic molecules are unknown, it is not possible to comment on their probable role in lens and how this may be affected by ouabain.…”

Section: Resultsmentioning

confidence: 99%

“…Since the chemical structures of these phosphatic molecules are unknown, it is not possible to comment on their probable role in lens and how this may be affected by ouabain. [9]. The quadratic expression y = Ax2 + Bx + C was em ployed to fit the time-course data for the incubation period.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Phosphorus-31 NMR Analysis of Dynamic Energy Metabolism in Intact Crystalline Lens Treated with Ouabain: Phosphorylated Metabolites

Greiner¹,

Kopp²,

Glonek³

1985

Ophthalmic Res

View full text Add to dashboard Cite

Changes in phosphatic metabolites and intralenticular pH of the intact crystalline rabbit lens induced by ouabain (10^––3, 10^––4, 10^––5M) were measured during time-course incubations using phosphorus-31 nuclear magnetic resonance spectroscopy (³¹P NMR) in order to study the metabolic manifestations of this steroid on lens glycolytic activity. Significant alterations in the glycolytic intermediates of the crystalline lens only occurred with ouabain at the concentration of 10^––3M. A significant time-dependent increase in α-glycerophosphate, an unidentified pentose phosphate, the nucleoside diphosphosugars (uridine diphosphoglucose and uridine diphosphomannose), inorganic orthophosphate, and inosine monophosphate, and decline in glucose 6-phosphate and ATP were detected in 10^––³M ouabain-treated lenses. The uridine diphosphosugars exhibited a maximum at approximately the time when the lens ATP content was 50% of control levels (12.5 h). A progressive decrease in intralenticular pH indicative of hydrogenion-pump damage was detected concomitant with the changes in lens metabolite levels. Lenses maintained transparency throughout the time-course. Since metabolite manifestations effecting the intermediates of glycolysis occur in lens tissue with its high glycolytic and low oxidative activity, we hypothesize the effects of 10^––3M ouabain may represent metabolic actions of ouabain which are independent of its effects on tissue oxygen consumption as is presumed from studies involving tissues more dependent on oxidative metabolism.

show abstract

Metabolomics

Alnouti¹

2013

Springer Protocols Handbooks

View full text Add to dashboard Cite

Altered metabolism and function of rat heart following chronic low level cadmium/lead feeding

Cited by 27 publications

References 24 publications

P‐31 Nuclear Magnetic Resonance Analysis of Brain: The Perchloric Acid Extract Spectrum

P‐31 Nuclear Magnetic Resonance Analysis of Brain: The Perchloric Acid Extract Spectrum

Phosphorus-31 NMR Analysis of Dynamic Energy Metabolism in Intact Crystalline Lens Treated with Ouabain: Phosphorylated Metabolites

Metabolomics

Contact Info

Product

Resources

About