Altered Membrane Structure and Surface Potential in Homozygous Hemoglobin C Erythrocytes

Tokumasu, Fuyuki; Nardone, Glenn; Ostera, Graciela R.; Fairhurst, Rick M.; Beaudry, Steven D.; Hayakawa, Eri; Dvorak, James A.

doi:10.1371/journal.pone.0005828

Cited by 23 publications

(17 citation statements)

References 62 publications

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“…Increased red cell membrane fragility has often been associated with membrane vesiculation in different hematological situations [19]–[21]. It is interesting to note that the clustering of AE1 through its binding to hemichromes has been demonstrated in most of these conditions [22]–[29]. Moreover, in hemolytic diseases, the accumulation of vesicles derived from the red cell membranes has received particular consideration because of their potential pro-coagulant and pro-inflammatory activities [30], [31].…”

Section: Discussionmentioning

confidence: 99%

Irreversible AE1 Tyrosine Phosphorylation Leads to Membrane Vesiculation in G6PD Deficient Red Cells

Pantaleo

Ferru

Carta³

et al. 2011

PLoS ONE

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BackgroundWhile G6PD deficiency is one of the major causes of acute hemolytic anemia, the membrane changes leading to red cell lysis have not been extensively studied. New findings concerning the mechanisms of G6PD deficient red cell destruction may facilitate our understanding of the large individual variations in susceptibility to pro-oxidant compounds and aid the prediction of the hemolytic activity of new drugs.Methodology/Principal FindingsOur results show that treatment of G6PD deficient red cells with diamide (0.25 mM) or divicine (0.5 mM) causes: (1) an increase in the oxidation and tyrosine phosphorylation of AE1; (2) progressive recruitment of phosphorylated AE1 in large membrane complexes which also contain hemichromes; (3) parallel red cell lysis and a massive release of vesicles containing hemichromes. We have observed that inhibition of AE1 phosphorylation by Syk kinase inhibitors prevented its clustering and the membrane vesiculation while increases in AE1 phosphorylation by tyrosine phosphatase inhibitors increased both red cell lysis and vesiculation rates. In control RBCs we observed only transient AE1 phosphorylation.Conclusions/SignificanceCollectively, our findings indicate that persistent tyrosine phosphorylation produces extensive membrane destabilization leading to the loss of vesicles which contain hemichromes. The proposed mechanism of hemolysis may be applied to other hemolytic diseases characterized by the accumulation of hemoglobin denaturation products.

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Section: Discussionmentioning

confidence: 99%

Irreversible AE1 Tyrosine Phosphorylation Leads to Membrane Vesiculation in G6PD Deficient Red Cells

Pantaleo

Ferru

Carta³

et al. 2011

PLoS ONE

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“…[41] Deviations of ζ potential of cells from the expected values have been used to conclude about their membrane abnormalities. [42] Charge on the cell membrane, originating from phosphoryl and carboxyl groups of macromolecules that constitute it, [43] can be manipulated to prevent cellular aggregation, which is an effect detrimental for cellular electrophoresis techniques. [44] It can also be assessed to differ between metabolically active and inactive cells, [45] as well as between rich, starved and dead ones.…”

Section: Other Applications Of the Concept Of ζ; Potential In Biochemmentioning

confidence: 99%

Dynamic Light Scattering Based Microelectrophoresis: Main Prospects and Limitations

Uskoković¹

2012

Journal of Dispersion Science and Technology

125

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Microelectrophoresis based on the dynamic light scattering (DLS) effect has been a major tool for assessing and controlling the conditions for stability of colloidal systems. However, both the DLS methods for characterization of the hydrodynamic size of dispersed submicron particles and the theory behind the electrokinetic phenomena are associated with fundamental and practical approximations that limit their sensitivity and information output. Some of these fundamental limitations, including the spherical approximation of DLS measurements and an inability of microelectrophoretic analyses of colloidal systems to detect discrete charges and differ between differently charged particle surfaces due to rotational diffusion and particle orientation averaging, are revisited in this work. Along with that, the main prospects of these two analytical methods are mentioned. A detailed review of the role of zeta potential in processes of biochemical nature is given too. It is argued that although zeta potential has been used as one of the main parameters in controlling the stability of colloidal dispersions, its application potentials are much broader. Manipulating surface charges of interacting species in designing complex soft matter morphologies using the concept of zeta potential, intensively investigated recently, is given as one of the examples. Branching out from the field of colloid chemistry, DLS and zeta potential analyses are now increasingly finding application in drug delivery, biotechnologies, physical chemistry of nanoscale phenomena and other research fields that stand on the frontier of the contemporary science. Coupling the DLS-based microelectrophoretic systems with complementary characterization methods is mentioned as one of the prosperous paths for increasing the information output of these two analytical techniques.

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“…Electrostatic effects have been regularly used for the electrophoretic separation of peptides, and the protein adsorption has been shown to be directly dependent on the magnitude of the difference between the ζ-potentials of the protein and the adsorbent8. Deviations of ζ-potential of cells from the normal range of values have been used as an indicator of membrane abnormalities9. Charge on the cell membrane, originating from phosphoryl and carboxyl groups of macromolecules that constitute it10, can be manipulated to prevent cellular aggregation, which is an effect detrimental for cellular electrophoresis techniques11.…”

Section: Introductionmentioning

confidence: 99%

Dynamic light scattering and zeta potential of colloidal mixtures of amelogenin and hydroxyapatite in calcium and phosphate rich ionic milieus

Uskoković¹,

Odsinada²,

Djordjevic³

et al. 2011

Archives of Oral Biology

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The concept of zeta-potential has been used for more than a century as a basic parameter in controlling the stability of colloidal suspensions, irrespective of the nature of their particulate ingredients – organic or inorganic. There are prospects that self-assembly of peptide species and the protein-mineral interactions related to biomineralization may be controlled using this fundamental physicochemical parameter. In this study, we have analyzed the particle size and zeta-potential of the full-length recombinant human amelogenin (rH174), the main protein of the developing enamel matrix, in the presence of calcium and phosphate ions and hydroxyapatite (HAP) particles. As calcium and phosphate salts are introduced to rH174 sols in increments, zeta-potential of the rH174 nanospheres is more affected by negatively charged ions, suggesting their tendency to locate within the double charge layer. Phosphate ions have a more pronounced effect on both the zeta-potential and aggregation propensity of rH174 nanospheres compared to calcium ions. The isoelectric point of amelogenin was independent on the ionic strength of the solution and the concentration of calcium and/or phosphate ions. Whereas rH174 shows a higher affinity for phosphate than for calcium, HAP attracts both of these ions to the shear plane of the double layer. The parallel size and zeta-potential analysis of HAP and rH174 colloidal mixtures indicated that at pH 7.4, despite both HAP and rH174 particles being negatively charged, rH174 adsorbs well onto HAP particles. The process is slower at pH 7.4 than at pH 4.5 when the HAP surface is negatively charged and the rH174 nanosphere carries an overall positive charge. The results presented hereby demonstrate that electrostatic interactions can affect the kinetics of the adsorption of rH174 onto HAP.

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Altered Membrane Structure and Surface Potential in Homozygous Hemoglobin C Erythrocytes

Cited by 23 publications

References 62 publications

Irreversible AE1 Tyrosine Phosphorylation Leads to Membrane Vesiculation in G6PD Deficient Red Cells

Irreversible AE1 Tyrosine Phosphorylation Leads to Membrane Vesiculation in G6PD Deficient Red Cells

Dynamic Light Scattering Based Microelectrophoresis: Main Prospects and Limitations

Dynamic light scattering and zeta potential of colloidal mixtures of amelogenin and hydroxyapatite in calcium and phosphate rich ionic milieus

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