Altered m6A modification is involved YAP-mediated apoptosis response in 4-vinylcyclohexene diepoxide induced ovotoxicity

Yang, Li; Li, Meifang; Liu, Jian; Nie, Guangning; Yang, Hongyan

doi:10.1016/j.ecoenv.2023.115192

Cited by 3 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wei et al recently found that VCD caused follicular atresia by in uencing the apoptosis of GCs [17]. Precious studies have shown that VCD is an ideal chemical agent for establishing POI models [18][19][20]. Current studies have shown that YLZ can protect ovarian function, effectively improve the ovarian function of POI rat models established by VCD and promote follicle development [10,12].…”

Section: Discussionmentioning

confidence: 99%

Yu Linzhu alleviates primary ovarian insufficiency by improving proliferation and energy metabolism of granulosa cells through HIF1α/Cx43 pathway

Ruan,

Wang,

Wei

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Yu Linzhu (YLZ) is a classical Chinese traditional fomula, which has been used more than 600 years regulating menstruation to help pregnancy. However, the mechanism of modern scientific action of YLZ needs to be further studied. Methods Thirty SD female rats were divided into three groups to prepare the blank serum and drug-containing serum, and then using UHPLC-QE-MS to identify the ingredients of YLZ and it’s drug-containing serum. Twenty-four SD female rats were divided four groups, except the control group, 4-vinylcyclohexene dicycloxide (VCD) was intraperitoneally injected to establish a primary ovarian insufficiency (POI) model of all groups. By using vaginal smear to show that the estrous cycle of rats was disturbed after modeling, indicate that the POI model was successfully established. The ELISA test was used to measure the follicle-stimulating hormone (FSH), estradiol (E2) and anti-Mullerian hormone (AMH) levels in the serum of rats. HE stain was used to assess the morphology of ovarian tissue. The localization and relative expression levels of CX43 protein were detected by tissue immunofluorescence. Primary ovarian granulosa cells (GCs) were identified by cellular immunofluorescence. CCK8 was used to screen time and concentration of drug-containing serum and evaluate the proliferation effect of YLZ on VCD-induced GCs. ATP kit and Seahorse XFe24 were used to detect energy production and real-time glycolytic metabolism rate of GCs. mRNA and protein expression levels of HIF1α, CX43, PEK, LDH, HK1 were detected by RT-PCR and WB. Results UHPLC-QE-MS found 1702 ingredients of YLZ and 80 constituents migrating to blood. YLZ reduced the FSH while increased the AMH and E2 levels. In ovarian tissues, YLZ improved ovarian morphology, follicle development and the relative expression of CX43. In vitro studies, we found that YLZ increased the proliferative activity of GCs, ATP levels, glycolytic metabolic rate, HIF1α, CX43, PEK, HK1, LDH mRNA and protein levels. Conclusions The study indicated that YLZ increased the proliferation and glycolytic energy metabolism of GCs to improve the follicular development so that further alleviated the ovarian function.

show abstract

Section: Discussionmentioning

confidence: 99%

Yu Linzhu alleviates primary ovarian insufficiency by improving proliferation and energy metabolism of granulosa cells through HIF1α/Cx43 pathway

Ruan,

Wang,

Wei

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The total m6A levels exhibited a significant decrease in the 4-Vinylcyclohexene diepoxide-treated mouse POF model, particularly in both preantral and antral follicles. Concurrently, there was an elevation in plasma folliclestimulating hormone levels and a reduction in AMH levels [168]. In addition, RNA modification may serve as a potential therapeutic target as well as a potential biomarker for POF.…”

Section: Rna Modifications and Pofmentioning

confidence: 92%

“…A significant decrease in total m6A levels observed in a POF rat model induced by 4-vinyl cyclohexene epoxide. Further, alterations in ALKBH5mediated YAP m6A modification were also observed [168]. One study showed that YTHDC2 expression was higher in late-stage ovaries, and that the disruption of YTHDC2 expression in mice led to impaired meiosis and an ovarian insufficiency phenotype [169].…”

Section: Rna Modifications and Pofmentioning

confidence: 99%

Role and Mechanism of Epigenetic Regulation in the Aging of Germ Cells: Prospects for Targeted Interventions

2024

Aging dis

View full text Add to dashboard Cite

In modern times, a notable trend toward delayed childbearing has been observed in most developed countries. As a result, sperm aging and quality loss, as well as premature ovarian failure (POF), have emerged as major causes of infertility. The pathogenesis of sperm aging and POF is complex and has not been clearly elucidated. However, evidence from some studies has linked germ cell aging to epigenetic modifications. Epigenetics refers to the heritable changes in gene expression that occur in the absence of any alterations to the gene's nucleotide sequence. This paper systematically reviewed and analyzed the relevant literature to describe the relationship of DNA methylation, non-coding RNA regulation, histone modifications, chromatin remodeling, and RNA modifications with sperm aging and POF. In addition, we analyzed how sperm aging and POF can be mitigated via epigenetic interventions. This review could provide new therapeutic insights and guide strategies for improving sperm quality and ovarian function.

show abstract

Yu Linzhu alleviates primary ovarian insufficiency in a rat model by improving proliferation and energy metabolism of granulosa cells through hif1α/cx43 pathway

Ruan,

Wang,

Wei

et al. 2024

J Ovarian Res

View full text Add to dashboard Cite

Background Yu Linzhu (YLZ) is a classical Chinese traditional formula, which has been used for more than 600 years to regulate menstruation to help pregnancy. However, the mechanism of modern scientific action of YLZ needs to be further studied. Methods Thirty SD female rats were divided into three groups to prepare the blank serum and drug-containing serum, and then using UHPLC-QE-MS to identify the ingredients of YLZ and its drug-containing serum. Twenty-four SD female rats were divided into four groups, except the control group, 4-vinylcyclohexene dicycloxide (VCD) was intraperitoneally injected to establish a primary ovarian insufficiency (POI) model of all groups. Using vaginal smear to show that the estrous cycle of rats was disturbed after modeling, indicates that the POI model was successfully established. The ELISA test was used to measure the follicle-stimulating hormone (FSH), estradiol (E2), and anti-Mullerian hormone (AMH) levels in the serum of rats. HE stain was used to assess the morphology of ovarian tissue. The localization and relative expression levels of CX43 protein were detected by tissue immunofluorescence. Primary ovarian granulosa cells (GCs) were identified by cellular immunofluorescence. CCK8 was used to screen time and concentration of drug-containing serum and evaluate the proliferation effect of YLZ on VCD-induced GCs. ATP kit and Seahorse XFe24 were used to detect energy production and real-time glycolytic metabolism rate of GCs. mRNA and protein expression levels of HIF1α, CX43, PEK, LDH, HK1 were detected by RT-PCR and WB. Results UHPLC-QE-MS found 1702 ingredients of YLZ and 80 constituents migrating to blood. YLZ reduced the FSH while increasing the AMH and E2 levels. In ovarian tissues, YLZ improved ovarian morphology, follicle development, and the relative expression of CX43. In vitro studies, we found that YLZ increased the proliferative activity of GCs, ATP levels, glycolytic metabolic rate, HIF1α, CX43, PEK, HK1, LDH mRNA, and protein levels. Conclusions The study indicated that YLZ increased the proliferation and glycolytic energy metabolism of GCs to improve follicular development further alleviating ovarian function.

show abstract

Altered m6A modification is involved YAP-mediated apoptosis response in 4-vinylcyclohexene diepoxide induced ovotoxicity

Cited by 3 publications

References 34 publications

Yu Linzhu alleviates primary ovarian insufficiency by improving proliferation and energy metabolism of granulosa cells through HIF1α/Cx43 pathway

Yu Linzhu alleviates primary ovarian insufficiency by improving proliferation and energy metabolism of granulosa cells through HIF1α/Cx43 pathway

Role and Mechanism of Epigenetic Regulation in the Aging of Germ Cells: Prospects for Targeted Interventions

Yu Linzhu alleviates primary ovarian insufficiency in a rat model by improving proliferation and energy metabolism of granulosa cells through hif1α/cx43 pathway

Contact Info

Product

Resources

About