Altered lipidomic profiles in patients with and without osteonecrosis of the femoral head after 1‐month glucocorticoid treatment

Wang, Xinyuan; Zhang, Lin‐Lin; Hua, Bingxuan; Ji, Zongfei; Wei, Fan Yan; Gong, Linjing; Zhu, Liang; Wang, Xiangdong; Yan, Zuoqin

doi:10.1002/ctm2.298

Cited by 9 publications

(10 citation statements)

References 10 publications

(14 reference statements)

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“… 22 , 23 , 24 Lipidomic changes are associated with severities, durations, stages, clinical phenomes, subtypes and prognosis of lung disease. The circulating levels of lipid elements have been suggested as biomarkers for metabolism‐based diagnosis, monitors for therapeutic effects and lipid‐oriented mechanisms, 25 , 26 , 27 , 28 although there are still clinical and technical obstacles to be overcome. Metabolic disorders of palmitic or stearic acids in lung cancer or normal epithelial cells influenced cell proliferation through regulation of lipid‐associated genes.…”

Section: Discussionmentioning

confidence: 99%

Regulatory roles of external cholesterol in human airway epithelial mitochondrial function through STARD3 signalling

Liu

et al. 2022

Clinical & Translational Med

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Background Hypercholesterolemia is found in patients with chronic lung inflammation, during which airway epithelial cells play important roles in maintenance of inflammatory responses to pathogens. The present study aims at molecular mechanisms by which cholesterol changes airway epithelial sensitivity in response to smoking. Methods Human bronchial epithelial cells (HBEs) were stimulated with cigarette smoke extract (CSE) and mice were exposed to CS/lipopolysaccharide (LPS) as models in vitro and in vivo. Severe COPD patients and healthy volunteers were also enrolled and the level of cholesterol in plasma was detected by metabolomics. Filipin III and elisa kits were used to stain free cholesterol. Mitochondrial function was detected by mitotracker green, mitotracker green, and Seahorse. Mitochondrial morphology was detected by high content screening and electron microscopy. The mRNA and protein levels of mitochondrial dynamics‐related proteins were detected by RT‐qPCR and Western blot,respectively. BODIPY 493/503 was used to stain lipid droplets. Lipidomics was used to detect intracellular lipid components. The mRNA level of interleukin (IL)‐6 and IL‐8 were detected by RT‐qPCR. Results We found that the cholesterol overload was associated with chronic obstructive pulmonary disease (COPD) and airway epithelia‐driven inflammation, evidenced by hypercholesterolemia in patients with COPD and preclinical models, alteration of lipid metabolism‐associated genes in CSE‐induced airway epithelia and production of ILs. External cholesterol altered airway epithelial sensitivity of inflammation in response to CSE, through the regulation of STARD3‐MFN2 pathway, cholesterol re‐distribution, altered transport and accumulation of cholesterol, activities of lipid transport regulators and disorder of mitochondrial function and dynamics. MFN2 down‐regulation increased airway epithelial sensitivity and production of ILs after smoking, at least partially by injuring fatty acid oxidation and activating mTOR phosphorylation. Conclusions Our data provide new insights for understanding molecular mechanisms of cholesterol‐altered airway epithelial inflammation and for developing diagnostic biomarkers and therapeutic targets to improve patient outcomes.

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Section: Discussionmentioning

confidence: 99%

Regulatory roles of external cholesterol in human airway epithelial mitochondrial function through STARD3 signalling

Liu

et al. 2022

Clinical & Translational Med

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“…1,2,[7][8][9] The clinical lipidomics platform integrates lipidomic profiles with clinical phenomes to uncover disease stage-, severity-, duration-and type-specific biomarkers. 10,11 The interaction between lipids and genes plays critical roles in expression and activation of gene-specific lipid proteins and enzymes and lipid metabolism-associated genes, as well as in multi-directional regulations. This Editorial highlights the value of lipid-gene interaction in the identification and development of biomarkers and targets and emphasizes the significance of inter-regulation between lipids and genes in innovation and application of precise therapies for patients.…”

Section: Lipids and Genes: Regulatory Roles Of Lipids In Rna Expressionmentioning

confidence: 99%

“…Growing evidence demonstrates that metabolites and molecules generated during disease‐associated lipid metabolism can be an important source to identify and develop new diagnostic biomarkers and therapeutic targets 1,2,7–9 . The clinical lipidomics platform integrates lipidomic profiles with clinical phenomes to uncover disease stage‐, severity‐, duration‐ and type‐specific biomarkers 10,11 . The interaction between lipids and genes plays critical roles in expression and activation of gene‐specific lipid proteins and enzymes and lipid metabolism‐associated genes, as well as in multi‐directional regulations.…”

mentioning

confidence: 99%

Lipids and genes: Regulatory roles of lipids in RNA expression

Wang

Powell

2022

Clinical & Translational Med

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Nuclear lipid metabolism and metabolites play important roles in regulation of lipid–lipid, lipid–gene, lipid–chromatin, lipid–membrane and lipid–protein interactions to maintain the nuclear microenvironment, three‐dimensional chromatin architecture, gene expression and transcription and biological function. This Editorial highlights the value of lipid–gene interaction in the identification and development of biomarkers and targets and emphasizes the significance of inter‐regulation between lipids and genes in innovation and application of precise therapies for patients. Regulatory functions of nuclear lipid dynamics and biophysics modify transcriptomic expression, and modification (lipotranscriptome) can be a new approach for discovery and development of disease‐specific diagnoses and therapies, although there are several challenges to be overcome. Better understanding of how lipid‐based changes of nuclear functions and transcriptomic profiles modify clinical phenomes will provide new insights to understand molecular mechanisms of diseases and to develop spatiotemporal molecular medicine diagnostics and therapeutics.

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“…1,2,[7][8][9] The clinical lipidomics platform integrates lipidomic profiles with clinical phenomes to uncover disease stage-, severity-, duration,-and typespecific biomarkers. 10,11 The interaction between lipids and genes plays critical roles in the expression and activation of gene-specific lipid proteins and enzymes and lipid metabolism-associated genes, as well as in multidirectional regulation. This editorial highlights the value of lipid-gene interaction in the identification and development of biomarkers and targets and emphasises the significance of interregulation between lipids and genes in the innovation and application of precise therapies for patients.…”

Section: Lipids and Genes: Regulatory Roles Of Lipids In Rna Expressionmentioning

confidence: 99%

“…Growing evidence demonstrates that metabolites and molecules generated during disease‐associated lipid metabolism can be an important source to identify and develop new diagnostic biomarkers and therapeutic targets 1,2,7–9 . The clinical lipidomics platform integrates lipidomic profiles with clinical phenomes to uncover disease stage‐, severity‐, duration,‐ and type‐specific biomarkers 10,11 . The interaction between lipids and genes plays critical roles in the expression and activation of gene‐specific lipid proteins and enzymes and lipid metabolism‐associated genes, as well as in multidirectional regulation.…”

mentioning

confidence: 99%

Lipids and Genes: Regulatory roles of lipids in RNA expression

Wang

Han

Powell

2022

Clinical and Translational Dis

View full text Add to dashboard Cite

Nuclear lipid metabolism and metabolites play important roles in the regulation of lipid‐lipid, lipid‐gene, lipid‐chromatin, lipid‐membrane, and lipid‐protein interactions to maintain the nuclear microenvironment, three‐dimensional chromatin architecture, gene expression and transcription, and biological function. This editorial highlights the value of lipid‐gene in the identification and development of biomarkers and targets and emphasises the significance of interregulation between lipids and genes in the innovation and application of precise therapies for patients. Regulatory functions of nuclear lipid dynamics and biophysics modify transcriptomic expression, and modification (lipotranscriptome) can be a new approach for discovery and development of disease‐specific diagnoses and therapies, although there are several challenges to be overcome. A better understanding of how lipid‐based changes in nuclear functions and transcriptomic profiles modify clinical phenomes will provide new insights to understand the molecular mechanisms of diseases and to develop spatiotemporal molecular medicine diagnostics and therapeutics.

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Altered lipidomic profiles in patients with and without osteonecrosis of the femoral head after 1‐month glucocorticoid treatment

Cited by 9 publications

References 10 publications

Regulatory roles of external cholesterol in human airway epithelial mitochondrial function through STARD3 signalling

Regulatory roles of external cholesterol in human airway epithelial mitochondrial function through STARD3 signalling

Lipids and genes: Regulatory roles of lipids in RNA expression

Lipids and Genes: Regulatory roles of lipids in RNA expression

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