Altered levels of the Taraxacum kok-saghyz (Russian dandelion) small rubber particle protein, TkSRPP3, result in qualitative and quantitative changes in rubber metabolism

Collins-Silva, Jillian; Nural, Aise Taban; Skaggs, Amanda; Scott, Deborah J.; Hathwaik, Upul; Woolsey, Rebekah; Schegg, Kathleen M.; McMahan, Colleen; Whalen, Maureen C.; Cornish, Katrina; Shintani, David K.

doi:10.1016/j.phytochem.2012.04.015

Cited by 77 publications

(63 citation statements)

References 53 publications

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“…The SRPP protein family is named for the association of these proteins with rubber particles in laticifer cells of plants that synthesize rubber, such as rubber tree, guayule (Parthenium argentatum), and Russian dandelion (Taraxacum brevicorniculatum; Collins-Silva et al, 2012;Hillebrand et al, 2012). The SRPPs share homology with the rubber elongation factor and rubber allergen protein, and there is evidence that SRPP isoforms may Figure 6.…”

Section: Discussionmentioning

confidence: 99%

“…play a role in rubber synthesis (Collins-Silva et al, 2012). For instance, RNA interference suppression of SRPPs in Russian dandelion caused a significant reduction in overall rubber production and rubber synthase activity in comparison with wild-type plants (Hillebrand et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…4). Given the level of polypeptide sequence identity and the general similarity of rubber particles and lipid droplets in the storage of hydrophobic compounds in plant cells, we hypothesized that avocado LDAP1 and LDAP2 and Arabidopsis At3g05500 might similarly associate with and stabilize TAG-containing lipid droplets analogous to how SRPPs bind to the surface and stabilize polyisoprenoid-containing rubber particles (Collins-Silva et al, 2012;Hillebrand et al, 2012).…”

Section: Ldap1 and Ldap2mentioning

confidence: 99%

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Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

et al. 2013

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Lipid droplets in plants (also known as oil bodies, lipid bodies, or oleosomes) are well characterized in seeds, and oleosins, the major proteins associated with their surface, were shown to be important for stabilizing lipid droplets during seed desiccation and rehydration. However, lipid droplets occur in essentially all plant cell types, many of which may not require oleosinmediated stabilization. The proteins associated with the surface of nonseed lipid droplets, which are likely to influence the formation, stability, and turnover of this compartment, remain to be elucidated. Here, we have combined lipidomic, proteomic, and transcriptomic studies of avocado (Persea americana) mesocarp to identify two new lipid droplet-associated proteins, which we named LDAP1 and LDAP2. These proteins are highly similar to each other and also to the small rubber particle proteins that accumulate in rubber-producing plants. An Arabidopsis (Arabidopsis thaliana) homolog to LDAP1 and LDAP2, At3g05500, was localized to the surface of lipid droplets after transient expression in tobacco (Nicotiana tabacum) cells that were induced to accumulate triacylglycerols. We propose that small rubber particle protein-like proteins are involved in the general process of binding and perhaps the stabilization of lipid-rich particles in the cytosol of plant cells and that the avocado and Arabidopsis protein members reveal a new aspect of the cellular machinery that is involved in the packaging of triacylglycerols in plant tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Ldap1 and Ldap2mentioning

confidence: 99%

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Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

et al. 2013

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“…In addition, six SRPP homologues have been identified in latex of other latex-producing plants: the guayule homologues of SRPP (GHS; [45]) and 5 dandelion homologues (TkSRPP1-5) are also present in the so-called "Russian" dandelion, Taraxacum koksaghyz a good rubber producer [46,47]. This species originates indeed from eastern Kazakhstan [48] and the 5 TkSRPPs were originally identified in a Russian dandelion EST collection [46].…”

Section: Genes Phylogeny and Regulation Of Gene Expressionmentioning

confidence: 99%

Hevea brasiliensis REF (Hev b 1) and SRPP (Hev b 3): An overview on rubber particle proteins

et al. 2014

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“…Homologs of Hevea SRPP also exist in the rubber-producing plants Parthenium argentatum (guayule) (GHS) [34], T. kok-saghyz (TkSRPP) [35] and L. sativa (lettuce) (LsSRPP) [26]. To investigate the abundance of Hevea antigen homologs in both rubber-producing and non-rubberproducing plants, we performed BLAST queries (https://blast.ncbi.nlm.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition

Dafoe¹,

Huang²,

Yang³

2017

J Biotechnol Biomater

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The annual plant Taraxacum kok-saghyz, commonly called Russian dandelion, has recently gained commercial attention as an alternative source to the rubber tree, Hevea brasiliensis, for natural rubber latex. While producing rubber of equivalent quality, the potential application of TKS latex for hypoallergenic products remains to be determined. In order to quantify the allergenicity of TKS latex proteins, we compared the extent of anti-Hevea latex polyclonal and monoclonal antibody cross-reactivity toward Hevea latex proteins to that of TKS latex proteins by quantitative ELISA methods and semi-quantitative image densitometry. ELISA measurement of polyclonal antibody recognition toward TKS latex proteins was on the order of one-tenth relative to equal amounts of Hevea latex protein, while recognition by the monoclonal antibody was below 2%. Immunoblots confirmed significantly reduced polyclonal antibody recognition toward TKS latex proteins, in the same range as the cross-reactivity exhibited toward the rubber-producing plant, Lactuca sativa and Glycine max, a non-rubber-producing plant. Despite the presence of cross-reactivity, these quantitative results support TKS as an alternative source of latex that may require lessintensive processing for the reduction of antigenic proteins in the manufacture of hypoallergenic natural rubber latex products.

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Altered levels of the Taraxacum kok-saghyz (Russian dandelion) small rubber particle protein, TkSRPP3, result in qualitative and quantitative changes in rubber metabolism

Cited by 77 publications

References 53 publications

Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

Hevea brasiliensis REF (Hev b 1) and SRPP (Hev b 3): An overview on rubber particle proteins

Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition

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