Altered levels of memory T cell subsets and common γc cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment

Ray, Anuradha; Munisankar, Saravanan; Bhootra, Yukti; Dolla, Chandrakumar; Thiruvengadam, Kannan; Nutman, Thomas B.; Babu, Subash

doi:10.1371/journal.pntd.0006481

Cited by 5 publications

(6 citation statements)

References 47 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL‐15 was discovered by its structural similarity to IL‐2 and is produced by multiple tissues 25 . Rajamanickam et al demonstrated that the levels of IL‐15 decreased in the sera of patients infected with S. stercoralis 26 . Our results showed no differences in IL‐15 production between all groups of alcoholics and non‐alcoholics, infected or not, with S. stercoralis .…”

Section: Discussionsupporting

confidence: 39%

“…25 Rajamanickam et al demonstrated that the levels of IL-15 decreased in the sera of patients infected with S. stercoralis. 26 Our results showed no differences in IL-15 production between all groups of alcoholics and non-alcoholics, infected or not, with S. stercoralis. In this context, it is possible that the consumed amount of alcohol that was not measured in the alcoholic patients may have modulated the immune response.…”

Section: S Stercoralis Infection In Immunocompromised Patientscontrasting

confidence: 46%

See 1 more Smart Citation

Modulation of circulating cytokine production in alcoholic patients infected with Strongyloides stercoralis

Souza

Oliveira

et al. 2023

Parasite Immunology

View full text Add to dashboard Cite

Strongyloidiasis control is associated with a Th2 immune response. However, alcohol ingestion plays an important role in modulating the immune system. The aim of this study is to evaluate the occurrence of Strongyloides stercoralis infection in alcoholic patients, the levels of circulating cytokines (IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-15 and IL-17), and its correlation with modulation of parasitic load in alcoholic individuals infected with S. stercoralis. A total of 336 alcoholic patients, treated at the Alcoholic Care and Treatment Center were included in this study. The cytokine levels were measured by a commercial ELISA in 80 sera divided into four groups with 20 individuals each: alcoholics infected (ASs+) and not infected (ASs-) with S. stercoralis and non-alcoholics infected (NASs+) and not infected (NASs-) with the helminth.S. stercoralis frequency in alcoholic patients was 16.1% (54/336). The parasitic load varied from 1 to 546 larvae/g of faeces, median and interquartile range (IQR) of 9 and 1.0-62.5 larvae/g of faeces, while in non-alcoholic individuals the parasitic load was less than 10 larvae/g of faeces. Levels of circulating IL-4 were significantly higher in ASs+ when compared with NASs-group (p < .05). An inverse correlation between serum levels of IFN-γ and parasitic load in alcoholic patients infected with S. stercoralis was observed (r = À601; p < 0.01). These results suggest that modulation of IFN-γ production occurs in alcoholic individuals with high parasitic burden.

show abstract

Section: Discussionsupporting

confidence: 39%

Section: S Stercoralis Infection In Immunocompromised Patientscontrasting

confidence: 46%

Modulation of circulating cytokine production in alcoholic patients infected with Strongyloides stercoralis

Souza

Oliveira

et al. 2023

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Absolute counts of lymphocyte populations were calculated based on the equation: Absolute number/mm 3 of Leukocytes subset = [percent of lymphocyte × total number of white blood cells per mm 3 ]/100). 15 …”

Section: Methodsmentioning

confidence: 99%

“…Total lymphocyte counts were obtained from the hematology profile and the percentage of gated lymphocytes by flow cytometry was used to calculate the absolute numbers of T cell subsets. Absolute counts of lymphocyte populations were calculated based on the equation: Absolute number/mm 3 of Leukocytes subset = [percent of lymphocyte × total number of white blood cells per mm 3 ]/100) 15 …”

Section: Methodsmentioning

confidence: 99%

Characterization of memory T cell subsets and common γ−chain cytokines in convalescent COVID-19 individuals

Ray

Kumar

Pandiaraj

et al. 2022

Journal of Leukocyte Biology

Self Cite

View full text Add to dashboard Cite

T cells are thought to be an important correlates of protection against SARS‐CoV2 infection. However, the composition of T cell subsets in convalescent individuals of SARS‐CoV2 infection has not been well studied. The authors determined the lymphocyte absolute counts, the frequency of memory T cell subsets, and the plasma levels of common γ−chain in 7 groups of COVID‐19 individuals, based on days since RT‐PCR confirmation of SARS‐CoV‐2 infection. The data show that both absolute counts and frequencies of lymphocytes as well as, the frequencies of CD4 + central and effector memory cells increased, and the frequencies of CD4 + naïve T cells, transitional memory, stem cell memory T cells, and regulatory cells decreased from Days 15–30 to Days 61–90 and plateaued thereafter. In addition, the frequencies of CD8 + central memory, effector, and terminal effector memory T cells increased, and the frequencies of CD8 + naïve cells, transitional memory, and stem cell memory T cells decreased from Days 15–30 to Days 61–90 and plateaued thereafter. The plasma levels of IL‐2, IL‐7, IL‐15, and IL‐21—common γc cytokines started decreasing from Days 15–30 till Days 151–180. Severe COVID‐19 patients exhibit decreased levels of lymphocyte counts and frequencies, higher frequencies of naïve cells, regulatory T cells, lower frequencies of central memory, effector memory, and stem cell memory, and elevated plasma levels of IL‐2, IL‐7, IL‐15, and IL‐21. Finally, there was a significant correlation between memory T cell subsets and common γc cytokines. Thus, the study provides evidence of alterations in lymphocyte counts, memory T cell subset frequencies, and common γ−chain cytokines in convalescent COVID‐19 individuals.

show abstract

“…Intestinal soil‐transmitted helminths (STHs) affect nearly one quarter of the world population and greatly contribute to chronic disease burdens in endemic populations [1, 2]. Although anthelminthic drug therapy is key to interrupting transmission, alone it is insufficient to effectively eliminate STHs, evidenced by the occurrence of high rates of reinfections in communities in endemic regions [3–5]. The development of effective anti‐helminth vaccines is therefore vital for the future elimination of parasitic helminths [6].…”

Section: Introductionmentioning

confidence: 99%

Immunization with excretory‐secretory molecules of intestinal nematodes induces antigen‐specific protective memory Th2 cell responses

2023

View full text Add to dashboard Cite

Parasitic nematodes infect more than 1 billion people in the global south. The development of effective antihelminthic vaccines is a crucial tool for their future elimination. Protective immune responses to nematodes depend on Gata3+ Th2 cells, which can also be induced by nematode‐released products. Whether these nematode products induce antigen‐specific long‐lived memory T cells and thereby confer protection against a challenge infection is not known yet. Hence, we set out to characterize the formation of memory Th2 cells induced by immunization with Heligmosomoides polygyrus excretory‐secretory (HES) products, infection‐induced versus immunization‐induced recall responses to a challenge infection, and whether HES‐induced memory T cells show protective properties following adoptive transfer. Our results show that 8 weeks postimmunization, HES induces long‐lived functional memory Th2 cells at the site of immunization in the peritoneal cavity. Following a H. polygyrus challenge infection, HES‐immunized mice display MHC‐II‐dependent antigen‐specific Th2 cytokine responses in the gut‐draining lymph nodes, comparable to those induced by a prior natural infection. Moreover, adoptive transfer of sorted memory CD4+ T cells from HES‐immunized donors reduces female worm fecundity following a challenge H. polygyrus infection in recipient mice, highlighting a protective role for immunization‐induced memory T cells.

show abstract

Altered levels of memory T cell subsets and common γc cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment

Cited by 5 publications

References 47 publications

Modulation of circulating cytokine production in alcoholic patients infected with Strongyloides stercoralis

Modulation of circulating cytokine production in alcoholic patients infected with Strongyloides stercoralis

Characterization of memory T cell subsets and common γ−chain cytokines in convalescent COVID-19 individuals

Immunization with excretory‐secretory molecules of intestinal nematodes induces antigen‐specific protective memory Th2 cell responses

Contact Info

Product

Resources

About