Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines

Debray, Henri; Zhu, Qi; Delannoy, Philippe; Montreuil, Jean; Duś, Danuta; Radzikowski, C; Christensen, B.; Kieler, J

doi:10.1002/ijc.2910370421

Cited by 28 publications

(14 citation statements)

References 14 publications

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“…The level of b1-6 branching was dependent on competition for substrate between GlcNAc transferases III and V, which play a role in invasion and cell attachment in the extravasation stage of metastasis (Yoshimura et al 1995). Similarly in human uroepithelial cell lines, increased branching of complex-type asparagine-linked oligosaccharides was correlated with the degree of transformation and invasiveness (Debray et al 1986). Loss or truncation of b1-6 branched oligosaccharides has multiple eects on cellular phenotypes, including decreased adhesion to vascular endothelial cells, increased adhesion to extracellular matrix proteins (Dennis et al 1982), reduced cellular motility and invasion of the extracellular matrix (Yagel et al 1990), reduced cellular response to autocrine growth stimulation (Dennis et al 1990;VanderElst and Dennis 1991) and increased susceptibility to natural killer cells (Ahrens and Ankel 1987;Dennis and Laferte 1985).…”

Section: Discussionmentioning

confidence: 94%

Elevated activity of N -acetylglucosaminyltransferase V in human hepatocellular carcinoma

Yao

Zhou

Jiang

et al. 1998

Journal of Cancer Research and Clinical Oncology

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Cell-surface glycoproteins are regarded as candidates for involvement in the spread of tumor cells. N-linked beta1-6 branched oligosaccharides may contribute directly to the malignant or metastatic phenotypes of tumor cells. Increased beta1-6 branching has been associated with an increased level of N-acetylglucosaminyltransferase V (GlcNAc transferase V), the glycosyltransferase that initiates the beta1-6 branching. In this report, 33 pathologically verified hepatocellular carcinoma (HCC) specimens, six non-cancerous tissues surrounding HCC and five normal liver specimens have been studied. We have quantified N-linked beta1-6 branched oligosaccharides indirectly by measuring GlcNac transferase V activity. The average GlcNac transferase V activities in hepatocellular carcinoma (HCC), noncancerous tissues surrounding HCC and normal liver tissues were 324.2 +/- 269.8, 84.8 +/- 20.7 and 7.0 +/- 6.2 pmol product h(-1) mg protein(-1) (P < 0.05) respectively. In addition, the activity was correlated with the TNM classification of HCC. The average activities of GlcNAc transferase V in stages T1, T2-3 and T4 were 77.6 +/- 57.8, 369.0 +/- 294.7 and 329.9 +/- 205.9 pmol product h(-1) mg protein h(-1) respectively (P < 0.05), showing that the activity of the enzyme in advanced HCC was higher than that in early HCC. Our preliminary results indicated that GlcNAc transferase V activity increased in human HCC and was correlated with its progression.

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Section: Discussionmentioning

confidence: 94%

Elevated activity of N -acetylglucosaminyltransferase V in human hepatocellular carcinoma

Yao

Zhou

Jiang

et al. 1998

Journal of Cancer Research and Clinical Oncology

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“…Affinity chromatography of the glycosylpeptide mixtures from MDCK-3 and ras-MDCK cells was performed on concanavalin A (Con A)-Sepharose and on Lens culinaris agglutinin (LCA)-Sepharose at room temperature, in accordance with Debray et al [13]. In the third set of cultures, cell surface glycopeptides of both cell types were released by trypsin-EDTA (10 min at 37°C; Gibco Europe, cat.…”

Section: Labeling and Isolation Of Glycosylpeptidesmentioning

confidence: 99%

Altered glycosylation in Madin-Darby canine kidney (MDCK) cells after transformation by murine sarcoma virus

Bruyneel

Hebray²,

Mets

et al. 1990

Clin Exp Metast

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The changes in glycosylation of an immortalized epithelial cell line (MDCK) before and after progression towards a more malignant phenotype have been studied. The parental MDCK-3 cells were immortalized after long-term passage in vitro and have shown no tendency for spontaneous acquisition of malignancy-related phenotypes such as tumorigenicity, invasion and metastasis. They conserved morphological and functional characteristics of the epithelial tissue of origin. The ras-MDCK cells acquired the fully malignant phenotype after transformation with a Harvey murine sarcoma virus; they were immortalized, invasive in vitro and produced invasive and also metastatic tumors after subcutaneous injection into nude mice. Using immobilized lectins and gel chromatography, before and after liberation of O-linked glycans from the peptide moieties and also after removal of terminal sialic acid, we have found differences in the glycosylpeptides of both whole cells and cell surface trypsinates from ras-MDCK cultures as compared to the parental MDCK-3 cultures: (i) more sialic acid in the N-linked tri- and tetra-antennary structures; (ii) more fucosylation in the N-glycosylpeptides; (iii) more bi-antennary N-glycosylpeptides and less O-linked glycans; and (iv) a lower molecular weight of the O-linked glycans probably due to a decreased sialylation. It is concluded that alterations in sialylation and fucosylation of the cell surface exposed glycans accompanied progression of MDCK-3 cells towards a more malignant phenotype.

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“…Normal (TGr0) and slightly transformed (TGrI) cells differ from pre-malignant (TGrII) and malignant (TGrIII) cells in their morphology and finite life-span in culture. TGrIII cells differ from TGrII cells in their morphology [2], their tumourigenicity in nude mice [2,19], in their ability to invade normal tissue in vitro [2,10], in the glycosylation of membrane glycoproteins [1] and in their apparent lack of polymorphic HLA-A,B epitopes [18]. In the present study we demonstrate an inverse relationship between the TGrII and TGrIII classification of 9 human urothelial cell lines and their expression of the monomorphic part of HLA-A,B,C.…”

Section: Offprint Requests To: S S Ottesenmentioning

confidence: 53%

Correlation between classification of human urothelial cell lines and HLA-A,B,C expression

Ottesen

Tromholt

Kieler

1988

Cancer Immunol Immunother

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Quantitative changes in major histocompatibility class I antigen expression in tumour cells are believed to affect the host immune response against the tumour. In tumourigenic (TGrIII) human urothelial cell lines the apparent loss of polymorphic HLA-A,B epitopes has previously been demonstrated. In the present study, 3 non-tumourigenic (TGrII) and 6 tumourigenic (TGrIII) human urothelial cell lines have been investigated for their quantitative expression of monomorphic HLA-A,B,C and B2-microglobulin. Evidence is provided that an inverse correlation exists between tumourigenicity and HLA-A,B,C and B2-microglobulin expression. Furthermore, treatment of the cells with neuraminidase partly restored the expression of monomorphic HLA-A,B,C suggesting that at least some of the observed quantitative differences could be due to masking of the membrane bound HLA antigens by sialic acid-containing glycoconjugates.

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Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines

Cited by 28 publications

References 14 publications

Elevated activity of N -acetylglucosaminyltransferase V in human hepatocellular carcinoma

Elevated activity of N -acetylglucosaminyltransferase V in human hepatocellular carcinoma

Altered glycosylation in Madin-Darby canine kidney (MDCK) cells after transformation by murine sarcoma virus

Correlation between classification of human urothelial cell lines and HLA-A,B,C expression

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