Altered glycosaminoglycan composition in reactive and neoplastic human liver

Kovalszky, Ilona; Pogány, Gábor; Molnár, Gergely; Jeney, A.; Lapis, K.; Karácsonyi, S; Szécsény, A; Iozzo, Renato V.

doi:10.1016/0006-291x(90)90606-n

Cited by 37 publications

(24 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase of the amounts of CS (Iozzo, 1985;Kovalszky et al, 1990;Tsara et al, 1995;Theocharis et al, 2000) and HA (Tsara et al, 1995;Theocharis et al, 2000;Knudson et al, 1989;Auvinen et al, 1997;Ropponen et al, 1998;Anttila et al, 2000) in malignant tissues is a common phenomenon, often related to their malignant phenotype and metastatic ability. This phenomenon is a challenge for the development of a reliable and valuable biochemical test, which will accompany and complete the histopathological examination for malignancies.…”

Section: Original Researchmentioning

confidence: 96%

High‐performance capillary electrophoretic analysis of hyaluronan and galactosaminoglycan‐disaccharides in gastrointestinal carcinomas. Differential disaccharide composition as a possible tool‐indicator for malignancies

Theocharis

2002

Biomedical Chromatography

View full text Add to dashboard Cite

The glycosaminoglycans (GAGs) have documented implications for the growth and progression of malignant tumors. Gastrointestinal carcinomas (gastric, colon, rectum and pancreatic) are the most frequent malignancies occurring in human. GAGs, isolated from the tissues after digestion with papain, were analyzed by high-performance capillary electrophoresis (HPCE) following treatment with chondroitinase ABC. The composition of GAGs in disaccharides derived from the various gastrointestinal carcinomas was compared with those of normal tissues. We report that human gastrointestinal carcinomas are characterized by increased concentrations of GAGs, which have quite different disaccharide composition which, in turn, is associated with marked increase of non-sulfated (Delta(di)-nonS) and 6-sulfated (Delta(di)-mono6S) Delta-disaccharides. Particularly, a 12-51-fold increase in Delta(di)-nonS and a 3-42-fold increase in Delta(di)-mono6S content characterize these carcinomas, while the 4-sulfated units (Delta(di)-mono4S) showed a lower increase, about 0.5-1.5-fold. Moreover, the quantitation of hyaluronan (HA)-derived Delta-disaccharides (Delta(di)-nonS(HA)) also revealed a marked increase (1-12-fold) in the malignant tissues. On the other hand, the content of the chondroitinase ABC-resistant GAGs showed a low decrease, about 0.2-0.7-fold. The high amounts of hyaluronan (HA) produced by these carcinomas and the ectopic production of chondroitin sulphate (CS) proteoglycans, in which (Delta(di)-nonS) and (Delta(di)-mono6S) predominated, suggest a close relation between the content of these GAGs and the malignant phenotype, the metastatic ability and the survival time.

show abstract

Section: Original Researchmentioning

confidence: 96%

High‐performance capillary electrophoretic analysis of hyaluronan and galactosaminoglycan‐disaccharides in gastrointestinal carcinomas. Differential disaccharide composition as a possible tool‐indicator for malignancies

Theocharis

2002

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Increased proportions of DDi-0S disaccharides have been observed in laryngeal (26), rectal (19), colon (7), liver (27), pancreatic (10), and head and neck tumor tissues (9) compared with normal tissue. DDi-0S disaccharide is the predominant disaccharide found in pancreatic carcinoma (10) and is increased 4-fold in gastric carcinoma compared with normal tissue (28).…”

Section: Cancer Epidemiology Biomarkers and Prevention Cancer Epidemiomentioning

confidence: 99%

Immunohistochemical Level of Unsulfated Chondroitin Disaccharides in the Cancer Stroma Is an Independent Predictor of Prostate Cancer Relapse

Sakko

Butler

Byers

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

The glycosaminoglycan chondroitin sulfate is significantly increased in the peritumoral stroma of prostate tumors compared with normal stroma and is an independent predictor of prostate-specific antigen (PSA) relapse following radical prostatectomy. In this study, we determined whether specific alterations in the sulfation pattern of glycosaminoglycan chains in clinically organ-confined prostate cancer are associated with PSA relapse. Immunoreactivity to distinct glycosaminoglycan disaccharide epitopes was assessed by manually scoring the staining intensity in prostate tissues from patients with benign prostatic hyperplasia (n = 19), early-stage cancer (cohort 1, n = 55 and cohort 2, n = 275), and advanced-stage cancer (n = 20). Alterations to glycosaminoglycans in benign and malignant prostate tissues were determined by cellulose acetate chromatography and high-pressure liquid chromatography. Glycosaminoglycan disaccharide epitopes were localized to the peritumoral stroma of clinically localized prostate cancer. The level of immunostaining for unsulfated disaccharides (C0S) in the peritumoral stroma, but not for 4-sulfated (C4S) or 6-sulfated disaccharides (C6S), was significantly associated with the rate of PSA relapse following radical prostatectomy. High levels of C0S immunostaining were determined to be an independent predictor of PSA relapse (1.6-fold, P = 0.020). Advanced-stage prostate cancer tissues exhibited reduced electrophoretic mobility for chondroitin sulfate and increased unsulfated disaccharides when compared with benign prostatic hyperplasia tissues, whereas the sulfated disaccharide levels were unaffected. The level of C0S immunostaining in the peritumoral stroma is an independent determinant of PSA failure in clinically localized prostate cancer. Specific alterations to chondroitin sulfate side chains occurring during tumor development may be a crucial step for disease progression in prostate cancer. (Cancer Epidemiol Biomarkers Prev 2008;17(9):2488 -97)

show abstract

“…An increase in HA and CS seems to be a characteristic abnormality of GAGs in hepatic carcinoma compared with normal liver tissue [23]. It has been reported that HCC shows a significant increase in undersulfated CS [24]. However, little is known about the relationship between GAG characteristics and histological types of primary hepatic carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Elevate Level of Glycosaminoglycans and Altered Sulfation Pattern of Chondroitin Sulfate Are Associated with Differentiation Status and Histological Type of Human Primary Hepatic Carcinoma

Sun

et al. 2007

Oncology

View full text Add to dashboard Cite

Objectives: The characteristics of glycosaminoglycans (GAGs) in many carcinomas have been reported to be different from those in normal tissues, which can be used as prognostic indices in some cancers. However, the difference in GAG characteristics among various differentiation status or histological types of the same cancer has not been described. The aim of this study was to investigate the relationship between GAG characteristics and human primary hepatic carcinomas of divers differentiation status or histological type. Methods: GAGs from intrahepatic cholangiocarcinomas and differently differentiated hepatocellular carcinomas were extracted, purified and enzymatically digested. Their content, relative molecular size distribution and disaccharide composition were analyzed and compared using electrophoresis and high-performance liquid chromatography. Results: A progressive increase in the content of chondroitin sulfate, low molecular size GAGs, and nonsulfated and disulfated chondroitin sulfate disaccharide units, together with a gradual decrease in heparan sulfate, have been found as the differentiation status of hepatocellular carcinoma became poorer. A significant increase in hyaluronic acid, which only slightly increased in hepatocellular carcinoma, was found in intrahepatic cholangiocarcinomas. Conclusion: The alterations in GAG characteristics in primary hepatic carcinoma were associated with both the differentiation status and the histological type of the tumor.

show abstract

Altered glycosaminoglycan composition in reactive and neoplastic human liver

Cited by 37 publications

References 18 publications

High‐performance capillary electrophoretic analysis of hyaluronan and galactosaminoglycan‐disaccharides in gastrointestinal carcinomas. Differential disaccharide composition as a possible tool‐indicator for malignancies

High‐performance capillary electrophoretic analysis of hyaluronan and galactosaminoglycan‐disaccharides in gastrointestinal carcinomas. Differential disaccharide composition as a possible tool‐indicator for malignancies

Immunohistochemical Level of Unsulfated Chondroitin Disaccharides in the Cancer Stroma Is an Independent Predictor of Prostate Cancer Relapse

Elevate Level of Glycosaminoglycans and Altered Sulfation Pattern of Chondroitin Sulfate Are Associated with Differentiation Status and Histological Type of Human Primary Hepatic Carcinoma

Contact Info

Product

Resources

About