Altered global methylation of DNA: An epigenetic difference in susceptibility for lung cancer is associated with its progression

Piyathilake, Chandrika J.; Frost, Andra R.; Bell, Walter C.; Oelschlager, Denise K.; Weiss, Heidi L.; Johanning, Gary L.; Niveleau, Alain; Heimburger, Douglas C.; Grizzle, William E.

doi:10.1053/hupa.2001.26471

Cited by 64 publications

(48 citation statements)

References 32 publications

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“…The recurrence of global DNA hypomethylation in many types of human cancer is suggestive of its significant role in carcinogenesis, perhaps by inducing genomic instability and/or activating oncogenes (8,9). However, global hypomethylation is subject to a high degree of variability, unaccounted for by our current level of understanding (10,11).…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 98%

Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

Székvölgyi

Imre

Minh

et al. 2009

Chromatin Immunoprecipitation Assays

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Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45-52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Balint et al. 2005. Mol. Cell Biol. 25, 5648-5663), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.

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Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 98%

Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

Székvölgyi

Imre

Minh

et al. 2009

Chromatin Immunoprecipitation Assays

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show abstract

“…In contrast, for lung, liver, and cervical cancers, significant increases in global hypomethylation of DNA have been observed with tumor progression. [32][33][34][35] In our previous investigations of DNA from ovarian epithelial carcinomas and Wilms tumors, 8,11,14 cancerspecific hypomethylation of centromeric Satα as well as juxtacentromeric Sat2 was seen when comparing tumors to various normal postnatal somatic tissues. Furthermore, hypomethylation at both Sat2 and Satα sequences depended on the degree of malignancy in a comparison of three types of ovarian epithelial neoplasms: carcinomas, borderline malignancies (low malignant potential tumors), and cystadenomas (benign tumors; Table 1; Ehrlich M, Dubeau L, unpublished data).…”

Section: B Amentioning

confidence: 99%

DNA hypomethylation is prevalent even in low-grade breast cancers

Jackson¹,

Yu²,

Arakawa³

et al. 2004

Cancer Biology & Therapy

108

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“…Although fibrosis may be a so-called precancerous lesion (42), DNA methylation patterns seems to have some differences between fibrosis and cancer. Hypomethylation of CpG sites is more frequently observed than hypermethylation in cancer and lesions from epithelial hyperplasia to lung cancer accompanied with decreased DNA methylation (18,43). Moreover, LINE-1 retrotransposon differs in methylation status of IPF and lung cancer (32).…”

Section: Measure the Pten And C-jun Methylation Status In Helfsmentioning

confidence: 99%

Global DNA methylation and PTEN hypermethylation alterations in lung tissues from human silicosis

et al. 2016

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Background: Silicosis is a respiratory disease caused by long-term silica dust exposure. Our previous study has demonstrated that silica mediates the activation of phosphatidylinositol 3-kinase (PI3K)/phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/serine or threonine kinase (AKT)/mitogen-activated protein kinases (MAPK)/AP-1 pathway in human embryo lung fibroblasts (HELFs). The purpose of this study is to identify genome-wide aberrant DNA methylation profiling in lung tissues from silicosis patients. Conclusions: Abnormal DNA methylation on genome-scale was implicated in silicosis, and PTEN promoter hypermethylation might be associated with decrease of PTEN protein.

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Altered global methylation of DNA: An epigenetic difference in susceptibility for lung cancer is associated with its progression

Cited by 64 publications

References 32 publications

Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

DNA hypomethylation is prevalent even in low-grade breast cancers

Global DNA methylation and PTEN hypermethylation alterations in lung tissues from human silicosis

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