Altered Gene Expression Profiles in Mouse Tetraploid Blastocysts

Park, Mi-Ryung; Hwang, Kyu‐Chan; Bui, Hong-Thuy; Cho, Ssang-Goo; Park, Chankyu; Song, Hyuk; Oh, Jae-Wook; Kim, Jin‐Hoi

doi:10.1262/jrd.11-110m

Cited by 8 publications

(5 citation statements)

References 25 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 Tetraploidderived blastocyst embryos had very few Oct4-positive cells at the mid-blastocyst stage and the inner cell mass biomarkers Oct4, Sox2 and Klf4 were expressed at less than 10% of the level observed in diploid blastocysts. 11 Interestingly, Recent work discovered that expression of Oct4 is lost in the tetraploid expanded blastocysts, whereas Cdx2 is strongly expressed at this stage. 12 It was reported that Ccnb1 (cyclin B1), which was reduced by 3-fold in tetraploid embryos, appears to play a particularly important role in the cellular organization of the tetraploid blastocyst.…”

Section: Introductionmentioning

confidence: 99%

Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice

Zhao

Zhu

et al. 2016

Cell Cycle

View full text Add to dashboard Cite

Polyploids are pervasive in plants and have large impacts on crop breeding, but natural polyploids are rare in animals. Mouse diploid embryos can be induced to become tetraploid by blastomere fusion at the 2-cell stage and tetraploid embryos can develop to the blastocyst stage in vitro. However, there is little information regarding mouse octaploid embryonic development and precise mechanisms contributing to octaploid embryonic developmental limitations are unknown. To investigate the genetic and epigenetic mechanisms underlying octaploid embryonic development, we generated mouse octaploid embryos and evaluated the in vitro/in vivo developmental potential. Here we show that octaploid embryos can develop to the blastocyst stage in vitro, but all fetus impaired immediately after implantation. Our results indicate that cell lineage specification of octaploid embryo was disorganized. Furthermore, these octaploid embryos showed increased apoptosis as well as alterations in epigenetic modifications when compared with diploid embryos. Thus, our cumulative data provide cues for why mouse octaploid embryonic development is limited and its failed postimplantation development.

show abstract

Section: Introductionmentioning

confidence: 99%

Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice

Zhao

Zhu

et al. 2016

Cell Cycle

View full text Add to dashboard Cite

show abstract

“…3,30 Tetraploid blastocysts have a functional trophectoderm but lack a functional ICM. 29 As such, tetraploid blastocysts are not independently capable of completing normal development but, when complemented by the introduction of ICM cells, they develop into mature fetuses in which embryonic lineages are derived entirely from the ICM cells, and extraembryonic lineages arise largely from the tetraploid component. This approach is often referred to as tetraploid complementation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Intrauterine Air Bubbles on Embryonic Development in Mice

Zhou

et al. 2019

j am assoc lab anim sci

View full text Add to dashboard Cite

Murine embryo transfer is the final and key step needed to produce offspring after in vitro fertilization or a transgenic procedure and is accomplished surgically or by transferring embryos into the oviduct or uterine horn of a pseudopregnant mouse. During the transfer of embryos into the uterine horn, air bubbles frequently are loaded with embryos into the transfer catheter. 26 However, the role of air bubbles on murine embryonic development is unclear. In addition, the issue has not been addressed in regard to embryo transfer procedures for humans and other animals. 5,31 In mice, the onset of pregnancy is heralded by the attachment of an embryo to the luminal epithelium and its penetration into the stroma of the endometrium. In response to the steroid hormones estrogen and progesterone, the stromal cells surrounding the implantation chamber undergo a remarkable transformation. This process, known as decidualization, is an essential prerequisite for successful implantation. 32 However, an implanting embryo is not absolutely required for endometrial decidualization. 12 Decidualization can occur in response to an artificial stimulus, such as small beads or droplets of oil injected into the uterine lumen. 2,24 The endometrial tissue that forms in response to an artificial deciduogenic stimulus is called a deciduoma to distinguish it from naturally induced decidua. Air bubbles reportedly can induce decidualization in mice, rats, and hamsters, 11,28 but whether air-induced decidualization disturbs decidual gene expression, implantation, or embryonic development is unclear currently. Materials and Methods Animals. Female (age, 8 to 12 wk) and male (age, 3 to 10 mo) CD1 mice (Beijing Vital River Laboratory Animal Technology, Beijing, China) were housed under constant environmental conditions (25 ± 1 °C; relative humidity, 40% to 60%; lights on, 0600 to 1800) at our institutional animal facility. Quarterly sentinel surveillance was used to screen for a wide range of pathogens, including epizootic diarrhea of infant mice, mouse hepatitis virus, mouse norovirus, mouse parvovirus 1 and 2, Mycoplasma pulmonis, pneumonia virus of mice, Sendai virus, Helicobacter spp., fur mites, and pinworms. All results from sentinel mice tested negative throughout this study. Mice received autoclaved feed (Beijing Vital River) and reverse-osmosis-deionized water without restriction. Mice were housed in polycarbonate microisolation caging with autoclaved hardwood bedding (Beijing Vital River). Autoclaved environmental enrichment was provided weekly to all mice in the form of 5-cm squares of cotton batting that could be shredded to form nests. All procedures in this study were approved by Hebei Agricultural University Laboratory Animal Care and Use Committee. Air-induced decidualization in pseudopregnant mice. Female CD1 mice were mated with vasectomized males. The next morning the female mice were examined for vaginal plugs, and the plug-positive females were considered to be at day 0.5 of pseudopregnancy. On day 3.5, between 1500 to 1700, ...

show abstract

“…Although their role has only marginally been elucidated so far ( Jia et al , 2015 ; Yu et al , 2022 ), several reports indicate that ER isoforms can differentially modulate estrogen signaling and, as a consequence, impact target gene regulation ( Ramsey et al , 2004 ; Elhasnaoui et al , 2021 ; Costa et al , 2022 ). Upon binding estrogen, ERα and ERβ dimerize and move to the cell nucleus, exerting their effect by activating or suppressing the transcription of a plethora of different genes, which are regulators of various physiological processes ( Mal et al , 2020 ) including uterine development and fertility, blastocyst implantation and the early stages of embryo development ( Park et al , 2012 ; Vasquez and DeMayo, 2013 ). Although responsible for several physiological functions in both females and males, studies performed on ERα and ERβ double knockout mice show that life is feasible even without E2 action, regardless of ubiquitous ER expression in almost all anatomical areas ( Lubahn et al , 1993 ; Dupont et al , 2000 ; Weihua et al , 2000 ).…”

Section: Estrogens and Their Receptorsmentioning

confidence: 99%

The pathophysiological role of estrogens in the initial stages of pregnancy: molecular mechanisms and clinical implications for pregnancy outcome from the periconceptional period to end of the first trimester

Parisi

Fenizia

Introini

et al. 2023

Human Reproduction Update

View full text Add to dashboard Cite

BACKGROUND Estrogens regulate disparate female physiological processes, thus ensuring reproduction. Altered estrogen levels and signaling have been associated with increased risks of pregnancy failure and complications, including hypertensive disorders and low birthweight babies. However, the role of estrogens in the periconceptional period and early pregnancy is still understudied. OBJECTIVE AND RATIONALE This review aims to summarize the current evidence on the role of maternal estrogens during the periconceptional period and the first trimester of pregnancies conceived naturally and following ART. Detailed molecular mechanisms and related clinical impacts are extensively described. SEARCH METHODS Data for this narrative review were independently identified by seven researchers on Pubmed and Embase databases. The following keywords were selected: ‘estrogens’ OR ‘estrogen level(s)’ OR ‘serum estradiol’ OR ‘estradiol/estrogen concentration’, AND ‘early pregnancy’ OR ‘first trimester of pregnancy’ OR ‘preconceptional period’ OR ‘ART’ OR ‘In Vitro Fertilization (IVF)’ OR ‘Embryo Transfer’ OR ‘Frozen Embryo Transfer’ OR ‘oocyte donation’ OR ‘egg donation’ OR ‘miscarriage’ OR ‘pregnancy outcome’ OR ‘endometrium’. OUTCOMES During the periconceptional period (defined here as the critical time window starting 1 month before conception), estrogens play a crucial role in endometrial receptivity, through the activation of paracrine/autocrine signaling. A derailed estrogenic milieu within this period seems to be detrimental both in natural and ART-conceived pregnancies. Low estrogen levels are associated with non-conception cycles in natural pregnancies. On the other hand, excessive supraphysiologic estrogen concentrations at time of the LH peak correlate with lower live birth rates and higher risks of pregnancy complications. In early pregnancy, estrogen plays a massive role in placentation mainly by modulating angiogenic factor expression—and in the development of an immune-tolerant uterine micro-environment by remodeling the function of uterine natural killer and T-helper cells. Lower estrogen levels are thought to trigger abnormal placentation in naturally conceived pregnancies, whereas an estrogen excess seems to worsen pregnancy development and outcomes. WIDER IMPLICATIONS Most current evidence available endorses a relation between periconceptional and first trimester estrogen levels and pregnancy outcomes, further depicting an optimal concentration range to optimize pregnancy success. However, how estrogens co-operate with other factors in order to maintain a fine balance between local tolerance towards the developing fetus and immune responses to pathogens remains elusive. Further studies are highly warranted, also aiming to identify the determinants of estrogen response and biomarkers for personalized estrogen administration regimens in ART.

show abstract

Altered Gene Expression Profiles in Mouse Tetraploid Blastocysts

Cited by 8 publications

References 25 publications

Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice

Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice

Effects of Intrauterine Air Bubbles on Embryonic Development in Mice

The pathophysiological role of estrogens in the initial stages of pregnancy: molecular mechanisms and clinical implications for pregnancy outcome from the periconceptional period to end of the first trimester

Contact Info

Product

Resources

About