Altered Gene Expression: Induction/Suppression in Leek Elicited by Iris Yellow Spot Virus Infection (IYSV) Egyptian Isolate

Hafez, Elsayed E.; Abdelkhalek, Ahmed; El-Wahab, Abeer Salah El-Deen Abd; Galal, Fatma H.

doi:10.5504/bbeq.2013.0068

Cited by 19 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results revealed that TMV induced a variation in gene expression within tomato tissues at all intervals. These results agree with previous observations [1,24,32,33]. All polymorphic bands were selected, excised from the agarose gel, purified and submitted for sequencing.…”

Section: Differential Display Pcr (Dd-pcr) and Up/down Regulated Genessupporting

confidence: 91%

“…To better clarify the molecular basis of tomato-TMV interactions with rapid identification of the differentially expressed tomato genes during viral infection, the DD-PCR technique was performed using five arbitrary primers (Table 1). Today, DD-PCR is increasingly effective in isolating and characterising genes differentially expressed amongst cells, tissues or individuals [1,22,24]. Many plants defend themselves against pathogens, such as viruses, bacteria and fungi, through altering their gene expressions [31].…”

Section: Resultsmentioning

confidence: 99%

“…The differential display-polymerase chain reaction (DD-PCR) technique provides a robust methodology for the fast identification of differentially expressed genes through scanning the differentially expressed mRNA in pathogen-infected plants [19][20][21][22]. Many studies on virus-host interactions use DD-PCR to isolate and characterise genes related to virus resistance [1,23,24]. Consequently, through using the DD-PCR technique, this study determined whether there were differentially expressed up-and/or down-regulated genes during tomato-TMV interactions, which are essential to better understand the tomato immune defence system against TMV infection.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A tomato kinesin-like protein is associated with Tobacco mosaic virus infection

Abdelkhalek

Ismail

Dessoky

et al. 2019

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Tobacco mosaic virus (TMV) causes severe damage and economic losses of tomato in crop yield production and quality worldwide. Tomato plants infected with TMV usually show a mosaic pattern and chlorosis symptoms that result from affected chloroplasts. To elucidate tomato-TMV interactions, the transcriptional profiles of tomato tissues at different time intervals were analysed using a differential display-polymerase chain reaction technique. Compared to mock-inoculated plants, and based on gene expression changes in tomato plants, the representative downand up-regulated genes were selected for further analysis. The Basic Local Alignment Search Tool at the National Center for Biotechnology Information (NCBI-BLAST) analysis revealed that the three up-regulated gene transcripts at 15 days post-inoculation were coding for chloroplast (MG565980 and MG565979) and kinesin-like proteins (MG565981). Additionally, the down-regulated gene was a chloroplast-related gene (MG565978). Interestingly, the two chloroplast genes, MG565978 and MG565980, shared more than 96% sequence identity, and their transmembrane profiles were nearly identical. On the other hand, many protein kinase C phosphorylation, casein kinase II phosphorylation and N-myristoylation sites were detected within the MG565981 gene. Moreover, the high similarity between the kinesin gene and many Arabidopsis defensin genes indicated that it might play an important role in the plant defence system against TMV infection. It should be stressed that studies of the pathways in which chloroplast and kinesin-like protein genes are involved may elucidate the mechanisms of tomato tolerance to viral infection and can lead us to a more comprehensive understanding of tomato-TMV interactions.

show abstract

Section: Differential Display Pcr (Dd-pcr) and Up/down Regulated Genessupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A tomato kinesin-like protein is associated with Tobacco mosaic virus infection

Abdelkhalek

Ismail

Dessoky

et al. 2019

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…Under insect-proof greenhouse conditions, tomato seeds were surface sterilized and cultivated in plastic pots (20 cm in diameter) filled with sterilized soil (clay: sand, 1:1). At 28th days post seedlings, tomato seedlings were transplanted into new pots, and one week later, two upper true leaves of each tomato plant were dusted with carborundum and mechanically inoculated with 1 mL of semi-purified TMV as previously described [43,44]. The experiment was carried out in five treatments, each treatment was comprised of five replicates, and each pot contained three tomato plants.…”

Section: Greenhouse Experimental Design and Antiviral Activity Assaymentioning

confidence: 99%

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

2020

View full text Add to dashboard Cite

Globally, plant viral infection is one of the most difficult challenges of food security, where considerable losses in crop production occur. Nanoparticles are an effective control agent against numerous plant pathogens. However, there is limited knowledge concerning their effects against viral infection. In the present study, the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous leaf extract of Mentha spicata was achieved. X-ray diffraction patterns confirmed the crystalline nature of the prepared ZnO NPs. Dynamic light scattering and scanning electron microscopy analyses revealed that the resultant ZnO NPs were spherical in shape with a particle size ranged from 11 to 88 nm. Fourier transmission infrared spectroscopy detected different functional groups, capping and stability agents, and showed Zn-O bond within wavenumber of 487 cm−1. Under greenhouse conditions, the antiviral activity of biological synthesized ZnO NPs (100 µg/mL) against Tobacco mosaic virus (TMV) was evaluated. The double foliar application of the prepared ZnO NPs, 24 h before and 24 h after TMV-inoculation, was the most effective treatment that showed a 90.21% reduction of viral accumulation level and disease severity. Additionally, the transcriptional levels of PAL, PR-1 (salicylic acid marker gene), CHS, and POD genes were induced and up-regulated in all ZnO NPs treated plants. Notably, the results exhibited that aqueous extract of Mentha spicata was an effective reducing agent for the green synthesis of ZnO NPs, which showed significant antiviral activity. Finally, the detected protective and curative activity of ZnO NPs against TMV can encourage us to recommend its application for plant viral disease management. To our knowledge, this is the first study describing the antiviral activity of the green synthesized ZnO NPs.

show abstract

“…The experiment was carried out in four treatments, each comprised five replicate pots, and one potato tuber/each pot. After 35 days of growing, two true upper leaves of each potato plants were dusted with carborundum and mechanically inoculated, as previously described 98 . The first treatment (T1) was mock-treated plants (control), in which potato plants inoculated with free-virus inoculation buffer + foliar spraying of free bacterial broth medium.…”

Section: Greenhouse Experimental Designmentioning

confidence: 99%

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Abdelkhalek

Al-Askar

Behiry

2020

Sci Rep

View full text Add to dashboard Cite

Alfalfa mosaic virus (AMV) is a worldwide distributed virus that has a very wide host range and causes significant crop losses of many economically important crops, including potato (Solanum tuberosum L.). In this study, the antiviral activity of Bacillus licheniformis strain POT1 against AMV on potato plants was evaluated. The dual foliar application of culture filtrate (CF), 24 h before and after AMV-inoculation, was the most effective treatment that showed 86.79% reduction of the viral accumulation level and improvement of different growth parameters. Moreover, HPLC analysis showed that a 20 polyphenolic compound was accumulated with a total amount of 7,218.86 and 1606.49 mg/kg in POT1-treated and non-treated plants, respectively. Additionally, the transcriptional analysis of thirteen genes controlling the phenylpropanoid, chlorogenic acid and flavonoid biosynthetic pathways revealed that most of the studied genes were induced after POT1 treatments. The stronger expression level of F3H, the key enzyme in flavonoid biosynthesis in plants, (588.133-fold) and AN2, anthocyanin 2 transcription factor, (97.005-fold) suggested that the accumulation flavonoid, especially anthocyanin, might play significant roles in plant defense against viral infection. Gas chromatography-mass spectrometry (GC-MS) analysis showed that pyrrolo[1,2-a]pyrazine-1,4-dione is the major compound in CF ethyl acetate extract, that is suggesting it acts as elicitor molecules for induction of systemic acquired resistance in potato plants. To our knowledge, this is the first study of biological control of AMV mediated by PGPR in potato plants.

show abstract

Altered Gene Expression: Induction/Suppression in Leek Elicited by Iris Yellow Spot Virus Infection (IYSV) Egyptian Isolate

Cited by 19 publications

References 47 publications

A tomato kinesin-like protein is associated with Tobacco mosaic virus infection

A tomato kinesin-like protein is associated with Tobacco mosaic virus infection

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Contact Info

Product

Resources

About