Altered Gene and Protein Expressions in Torn Rotator Cuff Tendon Tissues in Diabetic Patients

Chung, Seok Won; Choi, Bo Mi; Kim, Ja Yeon; Lee, Yong-Soo; Yoon, Jong Pil; Oh, Kyung-Soo; Park, Kyung Sik

doi:10.1016/j.arthro.2016.08.017

Cited by 29 publications

(21 citation statements)

References 43 publications

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“…Excess central adiposity is key constituent of MetS and adipose tissue is a rich source of circulating proinflammatory cytokines; collectively referred to as adipocytokines, including, tumour necrosis factor alpha (TNF-α), IL-1β, IL-1, IL-6, adiponectin, leptin and restinin. Increased cytokine expression has been observed in the subacromial bursa (IL-1 and IL-6) and partial thickness rotator cuff tear tissue (IL-6) from subjects undergoing rotator cuff surgery 77–79. Also, increased expression serum levels of TNF-α within subacromial bursa specimens have been retrieved during symptomatic rotator cuff tendon surgery 80.…”

Section: Discussionmentioning

confidence: 99%

Is there an association between metabolic syndrome and rotator cuff-related shoulder pain? A systematic review

Burne

Mansfield

Gaida

et al. 2019

BMJ Open Sport Exerc Med

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ObjectivesRotator cuff-related shoulder pain (RCRSP) is a common upper limb complaint. It has been suggested that this condition is more common among people with cardiometabolic risk factors. This systematic review has synthesised evidence from case–control, cross-sectional and cohort studies on the association between metabolic syndrome (MetS) and RCRSP.Design and data sourcesFive medical databases (MEDLINE, EMBASE, SCOPUS, CINAHL and AMED) and reference checking methods were used to identify all relevant English articles that considered MetS and RCRSP. Studies were appraised using the Newcastle-Ottawa Scale (NOS). Two reviewers performed critical appraisal and data extraction. Narrative synthesis was performed via content analysis of statistically significant associations.ResultsThree cross-sectional, two case–control and one cohort study met the inclusion criteria, providing a total of 1187 individuals with RCRSP. Heterogeneity in methodology and RCRSP or MetS definition precluded a meaningful meta-analysis. Four of the included studies identified associations between the prevalence of MetS and RCRSP. Studies consistently identified independent cardiometabolic risk factors associated with RCRSP. All studies were level III evidence.Summary and conclusionThe low-moderate quality evidence included in this review suggests an association between MetS and RCRSP. Most studies demonstrated moderate quality on appraisal. The direction of association and cardiometabolic factors influencing should be investigated by longitudinal and treatment studies. These preliminary conclusions and clinical utility should be treated with caution due to limitations of the evidence base.

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Section: Discussionmentioning

confidence: 99%

Is there an association between metabolic syndrome and rotator cuff-related shoulder pain? A systematic review

Burne

Mansfield

Gaida

et al. 2019

BMJ Open Sport Exerc Med

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“…To hallmark the tendon remodeling at the transcriptional level, we evaluated the expressional changes of Col1 , Col3 , and both MMP-2 and MMP-9 which have been associated with tendon remodeling elsewhere [8]. The present results show that the combination of LPBM and mechanical loading upregulated Col1 and Col3 mRNA expression after the 3-week experimental model.…”

Section: Discussionmentioning

confidence: 72%

“…While the pathogenesis of diabetic tendinopathy remains to be fully detailed [6], it is well known that the diabetic tendon exhibits a wide range of cellular, morphological, biomechanical and expressional alterations [1,7]. Among these alterations, collagen fiber disorganization and altered expression of key extracellular matrix (ECM) proteins, such as matrix metalloproteinases (MMPs) -2, -3, -9 and -13 [8] were previously reported as possible mechanisms for the impaired tendon healing in DM as they can lead to decreased degradation of matrix proteins and impaired tissue remodeling [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of photobiomodulation and exercise on early remodeling of the Achilles tendon in streptozotocin-induced diabetic rats

et al. 2019

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The aim of this study was to compare the treatment effects of laser photobiomodulation (LPBM) therapy and aerobic exercise on the biomechanical properties, tissue morphology and the expression of tendon matrix molecules during early remodeling of Achilles tendon (AT) injury in diabetic rats. Animals were randomly assigned to five groups: injured non diabetic (I, n = 15), injured diabetic (ID, n = 15), injured diabetic plus LPBM (IDL, n = 16), injured diabetic plus aerobic exercise (IDE, n = 16) and injured diabetic plus aerobic exercise and LPBM (IDEAL, n = 17). Type 1 diabetes was induced via a single intravenous injection of Streptozotocin at a dose of 40 mg/kg. A partial tenotomy was performed in the right AT. LPBM was performed with an indium-gallium-aluminum-phosphide 660 nm 10 mW laser device (spot size 0.04 cm2, power density 250 mW/cm2, irradiation duration 16 s, energy 0.16 J, energy density 4 J/cm2) on alternate days for a total of 9 sessions over 3 weeks (total energy 1.44 J), using a stationary contact technique to a single point over the dorsal aspect of the AT. Moderate aerobic exercise was performed on a motorized treadmill (velocity 9 m/min for 60 minutes). At 3 weeks post-injury, biomechanical analyzes as well as assessment of fibroblast number and orientation were performed. Collagen 1 (Col1) and 3 (Col3) and matrix metalloproteinases (MMPs) -3 and 13 protein distributions were studied by immunohistochemistry; while Col1 and Col3 and MMP-2 and 9 gene expression were assessed by quantitative RT-PCR (qRT-PCR). IDEAL exhibited significant increases in several biomechanical parameters in comparison to the other groups. Moreover, IDEAL presented stronger Col1 immunoreactivity when compared to ID, and weaker Col3 immunoreactivity than IDE. Both IDL and IDEAL demonstrated weaker expression of MMP-3 in comparison to I, while IDL presented no expression of MMP-13 when compared to ID. ID, IDL and IDE showed an increased number of fibroblasts in comparison to I, while IDEAL decreased the number of these cells in comparison to ID and IDE. IDL and IDEAL groups exhibited decreased angular dispersion among the fibroblasts when compared to I. The gene expression results showed that IDE demonstrated a downregulation in Col1 mRNA expression in comparison to I and ID. IDEAL demonstrated upregulation of Col1 mRNA expression when compared to IDL or IDE alone and increased MMP-2 expression when compared to IDL and IDE. MMP-9 expression was upregulated in IDEAL when compared to I, IDL and IDE. Our results suggest a beneficial interaction of combining both treatment strategies i.e., aerobic exercise and LPBM, on the biomechanical properties, tissue morphology and the expression of matrix molecules in diabetic tendons.

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“…Sejersen et al, 27 in a systematic review, identified 2,199 protein analysis studies in tendinopathies and verified a tendency to increase COL1, COL3, MMP1, MMP9, MMP13, TIMP1, VEGF expression and decrease in MMP3. Chung et al 28 found increased expression of MMP9 and IL6 genes in diabetics. The authors suggested that this difference may be one of the explanations for increased repair failure in diabetic patients.…”

Section: Rotator Cuff Injurymentioning

confidence: 98%

“…Sejersen et al 27 em revisão sistemática, identificaram 2.199 estudos de análise de proteínas em tendinopatias e verificaram uma tendência de aumento da expressão de COL1, COL3, MMP1, MMP9, MMP13, TIMP1, VEGF e diminuição de MMP3. Chung et al 28 encontraram o aumento da expressão dos genes MMP9 e IL6 em diabéticos. Os autores sugeriram que essa diferença pode ser uma das explicações para o aumento da falha do reparo em pacientes diabéticos.…”

Section: Lesão Do Manguito Rotadorunclassified

Aspectos genéticos nas afecções do ombro

Cohen

Figueiredo

Belangero

et al. 2020

Rev Bras Ortop (Sao Paulo)

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ResumoA influência da herança genética tem sido cada vez mais investigada nas afecções do ombro, como a lesão do manguito rotador, instabilidade e ombro congelado. Ainda que os achados iniciais sejam pouco esclarecedores, é necessário construir progressivamente um banco de marcadores genéticos para catalogar perfis genômicos que, mais adiante, poderão contribuir para a previsão do risco da doença, desenvolvimento de melhores ferramentas de diagnóstico e tratamento. O presente artigo busca atualizar o que há de evidências de estudos genéticos na literatura para essas doenças, desde análises de polimorfismos, expressão de genes candidatos em tecidos e estudos de associação genômica ampla (GWAS, na sigla em inglês). Porém, é necessário apontar que existe grande dificuldade na replicação e utilização dos achados, principalmente em razão da falta de poder estatístico, da alta taxa de resultados falso-positivos e da grande quantidade de variáveis envolvidas.

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Altered Gene and Protein Expressions in Torn Rotator Cuff Tendon Tissues in Diabetic Patients

Cited by 29 publications

References 43 publications

Is there an association between metabolic syndrome and rotator cuff-related shoulder pain? A systematic review

Is there an association between metabolic syndrome and rotator cuff-related shoulder pain? A systematic review

Effect of photobiomodulation and exercise on early remodeling of the Achilles tendon in streptozotocin-induced diabetic rats

Aspectos genéticos nas afecções do ombro

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