Altered Expression of Pro-inflammatory Cytokines in Ovarian Follicles of Cows with Cystic Ovarian Disease

Baravalle, María Eugenia; Stassi, Antonela Florencia; Velázquez, Melisa María del Luján; Belotti, Eduardo Matías; Rodríguez, Fernanda Mariel; Ortega, Hugo Héctor; Salvetti, Natalia Raquel

doi:10.1016/j.jcpa.2015.04.007

Cited by 25 publications

(27 citation statements)

References 58 publications

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“…However, few studies have suggested that a major infiltration of macrophages in the visceral, periovarian, or subcutaneous adipocytes may also be involved in the maturation of follicles in cows and mice [57, 58]. Our present findings show an important presence of CD68+ macrophages into the periovarian VAT of hypothyroid rabbits.…”

Section: Discussionsupporting

confidence: 60%

Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits

Rodríguez-Castelán

Méndez-Tepepa

Carrillo-Portillo

et al. 2017

BioMed Research International

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Ovarian failure is related to dyslipidemias and inflammation, as well as to hypertrophy and dysfunction of the visceral adipose tissue (VAT). Although hypothyroidism has been associated with obesity, dyslipidemias, and inflammation in humans and animals, its influence on the characteristics of ovarian follicles in adulthood is scarcely known. Control and hypothyroid rabbits were used to analyze the ovarian follicles, expression of aromatase in the ovary, serum concentration of lipids, leptin, and uric acid, size of adipocytes, and infiltration of macrophages in the periovarian VAT. Hypothyroidism did not affect the percentage of functional or atretic follicles. However, it reduced the size of primary, secondary, and tertiary follicles considered as large and the expression of aromatase in the ovary. This effect was associated with high serum concentrations of total cholesterol and low-density lipoprotein cholesterol (LDL-C). In addition, hypothyroidism induced hypertrophy of adipocytes and a major infiltration of CD68+ macrophages into the periovarian VAT. Our results suggest that the reduced size of ovarian follicles promoted by hypothyroidism could be associated with dyslipidemias, hypertrophy, and inflammation of the periovarian VAT. Present findings may be useful to understand the influence of hypothyroidism in the ovary function in adulthood.

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Section: Discussionsupporting

confidence: 60%

Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits

Rodríguez-Castelán

Méndez-Tepepa

Carrillo-Portillo

et al. 2017

BioMed Research International

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“…It has also been found to exert a negative effect on estrogen (E) production in human (36), rat (37) and bovine (38) GCs. Furthermore, altered expression of IL-6 may be related to ovulation failure and development of follicular cysts in cows (14) and may be capable of reducing LH-induced ovulation in rats (39). In the CL IL-6 decreased hCG-induced P secretion in porcine luteal cells (40).…”

Section: Discussionmentioning

confidence: 99%

“…Interleukin-8 was involved in follicular development and atresia, ovulation, steroidogenesis, and corpus luteum (CL) function (12), and inhibited estrogen (E) production and enhanced P production in bovine GCs (13). Tumor necrosis factor a (TNF-a) is a mediator of the immediate-early immune response (14) and, in particular, was demonstrated a powerful inhibitor of GC proliferation and gonadotropin-stimulated steroidogenesis in rats (15). The IL-6, IL-8, and TNF-a have also proven to have an important role in the oogenesis regulation (16).…”

mentioning

confidence: 99%

High cytokine expression and reduced ovarian reserve in patients with Hodgkin lymphoma or non-Hodgkin lymphoma

Paradisi

Vicenti

Macciocca

et al. 2016

Fertility and Sterility

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“…Another key dysregulation reported in cows with COF is the expression of immunological markers such as cytokines – key regulators of cell proliferation, follicular development, atresia, ovulation, steroidogenesis, and angiogenesis (13–15). Few investigations were performed with dairy cows, using either a model with induced follicle persistence or naturally occurring COF (3, 16, 17). The first study using a model of persistent follicles (5, 10 and 15 days of follicular persistence with day 0 as expected ovulation) revealed that interleukin 8 ( IL-8) and tumor necrosis factor-α ( TNF) was higher early (0 and/or 5 days of follicular persistence), and IL-6 was higher from 0 to 10 days of persistence, in relation to dominant follicles from the control group (3).…”

Section: Introductionmentioning

confidence: 99%

Steroidogenic, Metabolic, and Immunological Markers in Dairy Cows Diagnosed With Cystic Ovarian Follicles at Early and Mid-Late Lactation

et al. 2019

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The etiology of cystic ovarian follicles (COF) remains a conundrum with steroidogenic, immunological, and metabolic dysfunctions linked to its development. Studies suggest that COF development may occur as a result of disruption of the insulin signaling pathway and the severity of a negative energy balance in dairy cows, but mid to late lactation cows diagnosed with COF are unlikely to have issues with energy metabolism. Herein, we characterized the mRNA expression of steroidogenic (LHCGR, StAR, CYP11A1, 3β-HSD, CYP19A), immunological (IL-1β, IL-6, IL-8, TLR-4, TNF), and metabolic markers (IGF-1, IRS1) in follicular fluid; and plasma and follicular fluid levels of E2, IL-1β, glucose, and NEFA in early and mid-late lactation COF cows. Lactating dairy cows were diagnosed as having COF (n = 11, follicle >20 mm persistent for 7 days, absence of corpus luteum, and flaccid uterus) while 11 herdmates cycling with a dominant follicle were classified as the control cows. Cows diagnosed with COF were classified as early lactation (COF-E, n = 5) cows, <35 days in milk (DIM); or mid-late lactation (COF-M/L, n = 6), ≥118 DIM cows. Results revealed that mRNA expression StAR was greater (P < 0.01) in COF-E cows than COF-M/L cows and the control cows. The mRNA expression CYP19A1 was lower (P < 0.01) in COF-E cows and COF-M/L cows than in the control cows. The mRNA expression IL-6 and IRS-1 tended to be greater and lower, respectively, in COF-M/L cows compared to the control cows. The mRNA expression IGF-1 was greater (P < 0.01) in COF-E and COF-M/L cows than in the control cows. The plasma and follicular fluid concentration of NEFA was greater (P < 0.05) in COF-E cows than in COF-M/L and the control cows. Cows with COF-E had disturbances in steroidogenic and metabolic markers, while cows with COF-M/L had steroidogenic, immunological, and metabolic dysregulations, suggesting that COF pathogenesis may vary between early and mid-late lactation dairy cows.

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Altered Expression of Pro-inflammatory Cytokines in Ovarian Follicles of Cows with Cystic Ovarian Disease

Cited by 25 publications

References 58 publications

Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits

Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits

High cytokine expression and reduced ovarian reserve in patients with Hodgkin lymphoma or non-Hodgkin lymphoma

Steroidogenic, Metabolic, and Immunological Markers in Dairy Cows Diagnosed With Cystic Ovarian Follicles at Early and Mid-Late Lactation

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