Altered expression of OsNPF7.1 and OsNPF7.4 differentially regulates tillering and grain yield in rice

Huang, Weiting; Nie, Haipeng; Feng, Fan; Wang, Jie; Lü, Kai; Fang, Zhongming

doi:10.1016/j.plantsci.2019.01.019

“…Among the expression of N transporter genes, OsNPF2.4 was induced under 5.0 mM N, which is consistent with its functions in low-affinity acquisition and long-distance transport [58], supporting that OsNPF2.4 plays a role in promoting the axillary bud growth of plants grown under HN stress. In addition, the functions of many other reported genes were confirmed in our study, such as the functions of OsNPF7.1 [26], OsNPF7.2 [27], and OsNPF7.3 [29], which are differentially expressed in the basal parts or/and axillary buds in response to different N concentrations. Remarkably, many novel regulators were revealed, such as The dynamic transcriptomic data clearly demonstrated the responsive gene clusters and biological processes involved in the axillary bud growth of plants grown under various N supplies in detail.…”

Section: Expression Patterns Imply Spatio-temporal Specificity Of Gensupporting

confidence: 82%

“…N is a crucial macronutrient for plant development. Although previous studies have elucidated the regulatory mechanisms of the axillary bud growth under N stress [22,26,27,29,35,54], many regulators and their regulatory mechanisms are still unclear. This work revealed that the axillary bud growth rate was altered by different N concentrations, and integrated transcriptomic data from the basal parts and axillary buds of rice plants grown under different N concentrations via RNAseq.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

Wang

¹

,

Qian

²

,

Fang

³

et al. 2020

Preprint

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Background: N is an important macronutrient required for plant development and significantly influences axillary bud outgrowth, which affects tillering and grain yields of rice. However, how different N concentrations affect axillary bud growth at the molecular and transcriptional levels remains unclear. Results: In this study, morphological changes in the axillary bud growth of rice seedlings under different N concentrations ranging from low to high levels were systematically observed. To investigate the expression of N-induced genes involved in axillary bud growth, we used RNA-seq technology to generate mRNA transcriptomic data from two tissue types, basal parts and axillary buds, of plants grown under six different N concentrations. In total, 10,221 and 12,180 DEGs induced by LN or HN supplies were identified in the basal parts and axillary buds, respectively, via comparisons to expression levels under NN level. Analysis of the coexpression modules from the DEGs of the basal parts and axillary buds revealed an abundance of related biological processes underlying the axillary bud growth of plants under N treatments. Among these processes, the activity of cell division and expansion was positively correlated with the growth rate of axillary buds of plants grown under different N supplies. Additionally, TFs and phytohormones were shown to play crucial roles in determining the axillary bud growth of plants grown under different N concentrations. Further validation of OsGS1;2 and OsGS2 , the rice mutants of which presented altered tiller numbers, validated our transcriptomic data. Conclusion: These results indicate that different N concentrations affect the axillary bud growth rate, and our study revealed comprehensive expression profiles of genes that respond to different N concentrations, providing an important resource for future studies attempting to determine how axillary bud growth is controlled by different N supplies.

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“…Among the expression of N transporter genes, that of OsNPF2.4 was induced under 5.0 mM N, which is consistent with its functions in low-affinity acquisition and long-distance transport [60], supporting that OsNPF2.4 plays a role in promoting the axillary bud growth of plants grown under HN stress. In addition, the functions of many other reported genes were confirmed in our study, such as the functions of OsNPF7.1 [26], OsNPF7.2 [27], and OsNPF7.3 [28], which are differentially expressed in the basal parts or/and axillary buds in response to different N concentrations. Remarkably, many novel regulators were revealed, such as OsNPF8.20, OsAMT1.1/3.…”

Section: Regulatory Effects Of N Treatment On Axillary Bud Growth In supporting

confidence: 80%

“…N is a crucial macronutrient for plant development. Previous studies have elucidated the regulatory mechanisms of the axillary bud growth of plants grown under N stress [22,26,27,29,35,56]; however, many regulators and their regulatory mechanisms are still unclear. This work revealed that the growth rate of axillary buds was distinct in plants grown under different N concentrations, and integrated transcriptomic data from the basal parts and axillary buds of rice plants grown under six different N concentrations via RNA-sEq.…”

Section: Discussionmentioning

confidence: 99%

“…In rice, there are more than 80 NPF (NRT1/PTR), 4 NRT2 and 2 NAR2 genes [25], and their protein functions have been extensively studied. Recently, several genes involved in nitrate or peptide transport, such as OsNPF7.1 [26], OsNPF7.4 [26], OsNPF7.2 [27], OsNPF7.3 [28], and OsNPF7.7 [29], have been reported to mediate axillary bud outgrowth and tiller number in rice. Ammonium uptake is regulated by ammonium transporter/methylammonium permeases (AMT/MEPs), and ammonium is assimilated into glutamine (Gln) and glutamate (Glu) by glutamine synthetase (GS) and glutamate synthase (GOGAT) [30].…”

mentioning

confidence: 99%

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Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

Wang

¹

,

Qian

²

,

Fang

³

et al. 2019

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background: N is an important macronutrient required for plant development and significantly influences axillary bud outgrowth, which affects tillering and grain yields of rice. However, how different N concentrations affect axillary bud growth at the molecular and transcriptional levels remains unclear.Results: In this study, morphological changes in the axillary bud growth of rice seedlings under different N concentrations ranging from low to high levels were systematically observed. To investigate the expression of N-induced genes involved in axillary bud growth, we used RNA-seq technology to generate mRNA transcriptomic data from two tissue types, basal parts and axillary buds, of plants grown under six different N concentrations. In total, 10,221 and 12,180 DEGs induced by LN or HN supplies were identified in the basal parts and axillary buds, respectively, via comparisons to expression levels under NN level. Analysis of the coexpression modules from the DEGs of the basal parts and axillary buds revealed an abundance of related biological processes underlying the axillary bud growth of plants under N treatments. Among these processes, the activity of cell division and expansion was positively correlated with the growth rate of axillary buds of plants grown under different N supplies. Additionally, TFs and phytohormones were shown to play crucial roles in determining the axillary bud growth of plants grown under different N concentrations. Further validation of OsGS1;2 and OsGS2 , the rice mutants of which presented altered tiller numbers, validated our transcriptomic data.Conclusion: These results indicate that different N concentrations affect the axillary bud growth rate, and our study revealed comprehensive expression profiles of genes that respond to different N concentrations, providing an important resource for future studies attempting to determine how axillary bud growth is controlled by different N supplies. BackgroundRice is one of the three major crop species that provide food for more than half of the global population. Tiller number is an important agronomic trait that plays a role in rice yield by affecting the number of panicles per plant. The formation of rice tillers can be divided into two developmental 3 processes: axillary bud formation and outgrowth [1]. The axillary buds of tillers initiate in the axils of leaves of the basal part of shoots but then enter into dormancy. Later, these dormant axillary buds are activated and triggered by internal and environmental factors to outgrow to form tillers or branches. Therefore, both axillary bud formation and outgrowth are decisive factors that affect tiller number that contribute to grain yield.In the past few years, the regulatory mechanism of axillary bud formation and outgrowth governing

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Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses

Akhtar,

Ain,

Prasad

et al. 2024

The Plant Genome

0

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Nitrogen (N) as an inorganic macronutrient is inevitable for plant growth, development, and biomass production. Many external factors and stresses, such as acidity, alkalinity, salinity, temperature, oxygen, and rainfall, affect N uptake and metabolism in plants. The uptake of ammonium (NH4+) and nitrate (NO3−) in plants mainly depends on soil properties. Under the sufficient availability of NO3− (>1 mM), low‐affinity transport system is activated by gene network NRT1, and under low NO3− availability (<1 mM), high‐affinity transport system starts functioning encoded by NRT2 family of genes. Further, under limited N supply due to edaphic and climatic factors, higher expression of the AtNRT2.4 and AtNRT2.5T genes of the NRT2 family occur and are considered as N remobilizing genes. The NH4+ ion is the final form of N assimilated by cells mediated through the key enzymes glutamine synthetase and glutamate synthase. The WRKY1 is a major transcription factor of the N regulation network in plants. However, the transcriptome and metabolite profiles show variations in N assimilation metabolites, including glycine, glutamine, and aspartate, under abiotic stresses. The overexpression of NO3− transporters (OsNRT2.3a and OsNRT1.1b) can significantly improve the biomass and yield of various crops. Altering the expression levels of genes could be a valuable tool to improve N metabolism under the challenging conditions of soil and environment, such as unfavorable temperature, drought, salinity, heavy metals, and nutrient stress.

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Altered expression of OsNPF7.1 and OsNPF7.4 differentially regulates tillering and grain yield in rice

Cited by 38 publications

References 44 publications

Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses

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