Altered Expression of miRNAs Is Related to Larynx Cancer TNM Stage and Patients' Smoking Status

Abstract

It has been reported that microRNAs (miRNAs) are responsible for acquiring all the hallmarks of cancer cells, as well as have a significant impact on the clinical management of cancers at every stage, including prognosis, remission, relapse, and metastasis. In this study, we investigated the association of miR-29a-3p, miR-202-3p, miR-3713, miR-4768-3p, and miR-548aa expression with clinicopathologic features in patients suffering from laryngeal cancer (LC) and determined the potential role of studied miRNAs in the progression of LC. The study group consisted of 48 patients with untreated primary tumors of head and neck cancer localized in the larynx. Expression of the selected miRNAs was verified by the qRT-PCR technique. We showed that the expression of miR-29a as well as miR-548aa was positively correlated with tumor stage and lymph node metastasis, whereas the expression of miR-4768-3p was negatively correlated with lymph node metastasis. Furthermore, we investigated that exposure to cigarette smoke altered miRNA expression profile in LC. The expression level of miR-202-3p was significantly increased in smoking patients compared with nonsmokers, whereas the miR-4768-3p, miR-548aa, and miR-3713 were markedly decreased. Our research contributed toward better elucidating the mechanisms underlying the progression of LC as well as the use of miRNAs inhibitors as novel agents against progression and metastasis of LC.

MicroRNA‐384 regulates cell proliferation and apoptosis through directly targeting WISP1 in laryngeal cancer

“…Yilmaz SS et al found that miR‐221 was upregulated in LC plasma samples, and, in postoperative plasma samples, miR‐221 was at normal level. Bruzgielewicz et al showed that three miRs (miR‐4768‐3p, miR‐29a, and miR‐548a) were correlated with LC TNM stage and lymph node metastasis. Erkul E et al demonstrated that miR‐21 expression was at lower levels in early stage compared with advanced stage, implying that miR‐21 level may serve as a potential biomarker of LC diagnosis.…”

“…Yilmaz SS et al 16 found that miR-221 was upregulated in LC plasma samples, and, in postoperative plasma samples, miR-221 was at normal level. Bruzgielewicz et al 17 showed that F I G U R E 4 WISP1 knockdown inhibits LC cell proliferation and promotes cell apoptosis. TU212 and TU686 cells were, respectively, transfected with WISP1 siRNA (siWISP1) and its negative control (siNC), and then cultured for 24 hours.…”

Serum expression of selected miRNAs in patients with laryngeal squamous cell carcinoma (LSCC)

“…tissue rather than serum, or may be related to different world regions with their unique environmental factors or population characteristics [27]. Even smoking habit and alcohol consumption can significantly alter miRNA expression [28].…”

microRNAs deregulation in head and neck squamous cell carcinoma

“…Upregulation of miR‐31‐5p, miR‐29c‐3p, miR‐146a‐5p, miR193a‐5p, miR‐4454, and miR‐33b‐5p and downregulation of miR‐200 family and miR‐205 were found in tobacco‐treated oral carcinoma cell 55 . miR‐202‐3p was upregulated, whereas miR‐4768‐3p, miR‐548aa, and miR‐3713 were downregulated in smoking LSCC patients compared with nonsmoking patients 56 . miR‐133a‐3p was downregulated in HPV positive tumor of smokers OPSCC patients when compared to the nonsmoking group, facilitating tumorigenesis 57…”

“…Moreover, alcohol and smoke exposure can modulate miRNAs patterns in HNSCC 52‐57,170 . Alcohol induced the upregulation of miR‐375, 170 miR‐30a, miR‐934, 53 and miR‐34a, 52 promoting HNSCC pathogenesis and progression.…”