Altered expression of claudin-3 and claudin-4 in ectopic endometrium of women with endometriosis

Pan, Xiaoyu; Xue, Lin; Weng, Zhanping; Wang, Bo

doi:10.1016/j.fertnstert.2007.11.095

Cited by 27 publications

(39 citation statements)

References 41 publications

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“…Consistent with these findings, overexpression of these two claudin proteins has been positively correlated with tumor progression in the endometrium and increased myometrial invasion (10). In contrast to the overexpression of claudin-3 and -4 observed in endometrial cancer, endometriosis appears to be associated with a decrease in the levels of these two proteins (13,14). The reason for the upregulation of claudin-3 and -4 in certain endometrial tumors is currently unclear but given its role in the physiology of the endometrium, it is possible that E 2 may be involved.…”

Section: Introductionmentioning

confidence: 55%

Estrogen-dependent expression and subcellular localization of the tight junction protein claudin-4 in HEC-1A endometrial cancer cells

Cuevas

Gaska

Gist

et al. 2015

International Journal of Oncology

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Abstract. Endometrial cancer is the most common female reproductive cancer in the United States and is associated with deregulated tight junction protein expression. Given the highly estrogen-responsive nature of this tissue, we investigated the effects of estrogen and its agonist, 4-OH TAM, on the expression and subcellular localization of the tight junction protein claudin-4 (CLDN-4), in HEC-1A endometrial cancer cells. In untreated HEC-1A cells, we observed dramatic overexpression of claudin-4 protein. In addition, differential detergent extraction analysis indicated that claudin-4 was localized primarily in the membrane but also found in the cytosolic, nuclear and cytoskeletal fractions. Upon exposure of HEC-1A to estradiol (E 2 ), we observed a biphasic effect both on the overall expression of claudin-4 protein and on its cytosolic and cytoskeletal presence as demonstrated by immunoblot analysis. Immunofluorescence analysis also revealed a biphasic effect of E 2 on claudin-4 expression. In contrast, we observed no changes in expression levels nor in the subcellular distribution patterns of claudin-4 in HEC-1A cells treated with different concentrations of 4-OH TAM. The intracellular presence of CLDN-4 coupled with the biphasic effects of E 2 on CLDN-4 expression in the cytoskeleton suggest that this protein may be involved in cell signaling to and from TJs.

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Section: Introductionmentioning

confidence: 55%

Estrogen-dependent expression and subcellular localization of the tight junction protein claudin-4 in HEC-1A endometrial cancer cells

Cuevas

Gaska

Gist

et al. 2015

International Journal of Oncology

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“…This impairment of differentiation may affect the epithelial-to-mesenchymal or mesenchymal-to-epithelial transition in the endometrial tissue [103], which physiologically is accompanied by a highly regulated expression pattern of intercellular junctional complexes. This has been supported by a morphometric study showing that tight junctions were missing or disrupted in endometrioma compared to eutopic endometrium [39]. In a more recent study, microarray analyses revealed an upregulation of transcripts of JAM-B and JAM-C and of claudin-1, -5 and -11 and a downregulation of ZO-3, occludin and claudin-3, -4 and -7 in peritoneal endometriotic lesions compared to the corresponding eutopic endometrium [104].…”

Section: Direct Cell–cell Interactions In Endometrial Pathophysiologymentioning

confidence: 67%

“…However, these observations were not validated by PCR or immunohistochemical staining. In accordance with these findings, Pan and colleagues noted a significantly lower expression of claudin-3 and -4 in ovarian endometrioma compared to eutopic endometrium originating from patients with endometriosis and from healthy controls on mRNA and protein level [39]. In contrast, immunohistochemical analysis showed a decreased staining for claudins-1 and -5 in epithelial cells of peritoneal endometriotic lesions [36].…”

Section: Direct Cell–cell Interactions In Endometrial Pathophysiologymentioning

confidence: 76%

“…In contrast, the group of Sobel described a significant upregulation only of claudin-4 transcripts and an increase in claudin-1 and -5 protein in the secretory phase [35]. In contrast to that, other groups did not observe any regulation of claudin-3 and -4 throughout the menstrual cycle [33,37,39]. Interestingly, in women undergoing IVF treatment, the absence of claudin-4 in the presence of leukemia inhibitory factor (LIF) in the endometrium could be correlated with a 6-fold higher probability of successful establishment of pregnancy compared to samples that exhibited a strong claudin-4 and a weak LIF expression [40].…”

Section: Cell–cell Junctions In the Cyclic Human Endometriummentioning

confidence: 99%

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Direct Cell–Cell Interactions in the Endometrium and in Endometrial Pathophysiology

Grund

Grümmer

2018

IJMS

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Cell contacts exhibit a considerable influence on tissue physiology and homeostasis by controlling paracellular and intercellular transport processes, as well as by affecting signaling pathways. Since they maintain cell polarity, they play an important role in cell plasticity. The knowledge about the junctional protein families and their interactions has increased considerably during recent years. In contrast to most other tissues, the endometrium undergoes extensive physiological changes and reveals an extraordinary plasticity due to its crucial role in the establishment and maintenance of pregnancy. These complex changes are accompanied by changes in direct cell–cell contacts to meet the various requirements in the respective developmental stage. Impairment of this sophisticated differentiation process may lead to failure of implantation and embryo development and may be involved in the pathogenesis of endometrial diseases. In this article, we focus on the knowledge about the distribution and regulation of the different junctional proteins in the endometrium during cycling and pregnancy, as well as in pathologic conditions such as endometriosis and cancer. Decoding these sophisticated interactions should improve our understanding of endometrial physiology as well as of the mechanisms involved in pathological conditions.

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“…Interestingly, in a group of women with idiopathic infertility and with eutopic endometrium, the expression of claudin-4 was upregulated as compared to the control (fertile) group [36]. On the other hand, a decreased expression of claudin-3 and claudin-4 is associated with endometriosis [37], which is known for its significant contribution to the reduction of fertility. Claudin-7 is also reported to participate in fertilization.…”

Section: Uterusmentioning

confidence: 98%

Claudins: New Players in Human Fertility and Reproductive System Cancers

Kozieł

Kowalska

Piastowska-Ciesielska

2020

Cancers

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Claudins are major integral proteins of tight junctions (TJs), the apical cell-cell adhesions that enable maintaining polarity of epithelial cells, their differentiation, and cell signaling. A number of studies have indicated that claudins might play a crucial role in both physiology and pathogenesis. Their tissue-specific expression was originally linked to the development of different types of cancer and triggered a hope to use them as diagnostic or prognostic markers. However, it seems that their expression is more complex than that, and undoubtedly, claudins participate in one of the most important molecular events in cells. This review summarizes the recent research evaluating the role of claudins in fertility and the most common endocrine-dependent cancers in the reproductive system and highlights the crucial role of claudins both in human fertility and the most common cancers.

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Altered expression of claudin-3 and claudin-4 in ectopic endometrium of women with endometriosis

Cited by 27 publications

References 41 publications

Estrogen-dependent expression and subcellular localization of the tight junction protein claudin-4 in HEC-1A endometrial cancer cells

Estrogen-dependent expression and subcellular localization of the tight junction protein claudin-4 in HEC-1A endometrial cancer cells

Direct Cell–Cell Interactions in the Endometrium and in Endometrial Pathophysiology

Claudins: New Players in Human Fertility and Reproductive System Cancers

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