Altered expression of ADM and ADM2 by hypoxia regulates migration of trophoblast and HLA-G expression†

Gu, Changdai; Park, Sohae; Seok, Jin; Jang, Hee Yeon; Bang, Yong Ju; Kim, G I Jin

doi:10.1093/biolre/ioaa178

Cited by 12 publications

(6 citation statements)

References 31 publications

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“…Moreover, it is used as a target for diagnosis in clinical trials 33 . ADM is involved in immune regulation and trophoblast invasion under hypoxic conditions 34 . ARRB1 has been demonstrated to be a potential tumor promoter in prostate cancer and is important for metabolic alterations in prostate cancer 35 .…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Combined Hypoxia and Immune Prognostic Classifier for Lung Adenocarcinoma

et al. 2022

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Lung cancer is the leading cause of cancer-related deaths worldwide. Hypoxia is a crucial microenvironmental factor in lung adenocarcinoma (LUAD). However, the prognostic value based on hypoxia and immune in LUAD remains to be further clarified. The hypoxia-related genes (HRGs) and immune-related genes (IRGs) were downloaded from the public database. The RNA-seq expression and matched complete clinical data for LUAD were retrieved from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. The least absolute shrinkage and selection operator (LASSO) Cox regression analysis was applied to model construction. Hypoxia expression profiles, immune cell infiltration, functional enrichment analysis, Tumor Immune Dysfunction and Exclusion (TIDE) score and the somatic mutation status were analyzed and compared based on the model. Moreover, immunofluorescence (IF) staining in human LUAD cases to explore the expression of hypoxia marker and immune checkpoint. A prognostic model of 9 genes was established, which can divide patients into two subgroups. There were obvious differences in hypoxia and immune characteristics in the two groups, the group with high-risk score value showed significantly high expression of hypoxia genes and programmed death ligand-1 (PD-L1), and maybe more sensitive to immunotherapy. Patients in the high-risk group had shorter overall survival (OS). This model has a good predictive value for the prognosis of LUAD. We constructed a new HRGs and IRGs model for prognostic prediction of LUAD. This model may benefit future immunotherapy for LUAD.

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Section: Discussionmentioning

confidence: 99%

Development and Validation of a Combined Hypoxia and Immune Prognostic Classifier for Lung Adenocarcinoma

et al. 2022

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“…In 1997, HLA-G was first proved to have a protective effect on the fetus after semi-allogeneic transplantation in the maternal immune system, which was also supported in pathological research by Yie et al ( 23 ). Nowadays, HLA-G is considered to be a major immune checkpoint molecule and plays a crucial role in maternal-fetal immune tolerance ( 24 , 25 ). HLA-G can increase the activation threshold of immune cells before immune response by up-regulating the expression of inhibitory receptors on immune cells such as dNK cells and decidual T cells ( 26 – 28 ).…”

Section: Immunomodulatory Effect Of Hla-gmentioning

confidence: 99%

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

et al. 2022

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The HLA class Ib molecule is an alloantigen that causes transplant rejection on behalf of individual human and plays an important role in maternal-fetal immune tolerance. Early studies on HLA class Ib focused on the mechanism of HLA-G-induced immune escape, but in recent years, studies on the mechanism of HLA-G have deepened and gradually explored the mechanism of HLA-E and HLA-F, which are also HLA class Ib molecules. In the maternal-fetal interface, trophoblast cells express HLA class Ib molecules to protect the fetus from maternal immune cells by binding to inhibitory receptors of decidual immune cells (DICs) and shifting Th1/Th2 balance toward Th2 bias. Further studies on the molecular mechanism of HLA class Ib molecules provide a reference for its application in the field of clinical assisted reproduction.

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“…And some research showed that low O 2 levels can also promote EVT invasion. [8,[10][11][12][13] Therefore, a physiological hypoxic environment exists at the maternal-fetal interface during early pregnancy. However, there is a pathological hypoxic microenvironment in patients with PE, and the underlying pathological mechanisms remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

Liu

Wang

et al. 2023

Medicine

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Preeclampsia (PE) is a common pregnancy complication, and placental hypoxia is one of its causes. We aimed to identify the transcriptional profile and construct a long non-coding RNAs (lncRNA)-centered competing endogenous RNAs (ceRNA) network in hypoxia-induced HTR8/SVneo cells. We used datasets from the GEO database to identify important pathways in PE. We performed microarray profiling and functional analysis to identify differentially expressed long non-coding RNAs (lncRNAs), differentially expressed profiles of microRNA (miRNAs), and differentially expressed profiles of messenger RNA (mRNAs) in hypoxia-induced HTR8/SVneo cells. The candidates were validated using quantitative reverse transcription polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses were performed to understand the functional significance of differentially expressed genes. Finally, we constructed an lncRNA-centered ceRNA network. Several hub genes were validated both in placentas from PE and normal pregnancy, and in hypoxia-induced HTR8/SVneo cells. The hypoxic response pathway was involved in the pathophysiology of PE. Subsequently, we identified 536 differentially expressed profiles of lncRNAs (183 upregulated and 353 downregulated), 46 differentially expressed profiles of miRNAs (35 upregulated and 11 downregulated), and 2782 differentially expressed profiles of mRNAs (DEmRNAs) (1031 upregulated and 1751 downregulated) in hypoxia-induced HTR8/SVneo cells. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed potential pathways affected by these genes, such as angiogenesis, the HIF-1 signaling pathway, and the PI3K-Akt signaling pathway. The ceRNA network comprised 35 lncRNAs, 11 miRNAs, 27 mRNAs, and 2 hub lncRNAs, which might play a vital role in placental functions and PE. Our results revealed the transcriptome profile and constructed an lncRNA-centered ceRNA network in hypoxiainduced HTR8/SVneo cells, thereby providing potential therapeutic targets for PE.Abbreviations: BP = biological process, ceRNAs = competing endogenous RNAs, CTBs = cytotrophoblast cells, DEmiRNAs = differentially expressed profiles of miRNAs, DEmRNAs = differentially expressed profiles of mRNAs, EVT = extravillous trophoblast, GO = gene ontology, KEGG = Kyoto Encyclopedia of Genes and Genomes, lncRNAs = long non-coding RNAs, MEF2 = myocyte enhancer factor-2, MEG3 = lncRNA maternally expressed gene 3, messenger RNA = mRNA, microRNA = miRNA, PE = preeclampsia, qRT-PCR = quantitative reverse transcription polymerase chain reaction, SNHG16 = small nucleolar RNA host gene 16.

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Altered expression of ADM and ADM2 by hypoxia regulates migration of trophoblast and HLA-G expression†

Cited by 12 publications

References 31 publications

Development and Validation of a Combined Hypoxia and Immune Prognostic Classifier for Lung Adenocarcinoma

Development and Validation of a Combined Hypoxia and Immune Prognostic Classifier for Lung Adenocarcinoma

Advances in the study of HLA class Ib in maternal-fetal immune tolerance

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

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