Altered expression and glycosylation of plasma proteins in rheumatoid arthritis

Raghav, Sunil K.; Gupta, Bhawna; Agrawal, Charu; Saroha, Ashish; Das, Rakha H.; Chaturvedi, Ved; Das, Hasi R.

doi:10.1007/s10719-006-7922-6

Cited by 27 publications

(27 citation statements)

References 21 publications

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“…Another major glycoprotein, transferrin, accounts for a small proportion of the tri-antennary oligosaccharides found in healthy adults [22], and IgG and haptoglobin reportedly exhibit bi-antennary [23,24] rather than tri-antennary oligosaccharides. However, alteration in AGP N-glycans in RA is reported to involve only fucosylation, and proteomic analysis of serum in RA using 2-D PAGE and lectins shows changes in IgG, haptoglobin, and two unknown glycoproteins, but not in AGP [8]. Thus, the observed increase in tri-antennary oligosaccharides may occur in glycoproteins other than AGP, supporting the importance of analyzing whole serum N-glycans rather than just oligosaccharides on purified glycoproteins.…”

Section: Discussionmentioning

confidence: 93%

“…One particularly dramatic oligosaccharide change associated with disease is reduced galactose in IgG N-glycans seen in patients with rheumatoid arthritis (RA) [1]. Fucosylation of IgG [2] and N-glycan microheterogeneities in α1-acid glycoproteins (AGP) [3,4], transferrin [5], haptoglobin [6], α2-macrogloblin [7], and other plasma proteins [8] have also been reported in RA, although it is not known how alterations are associated with symptoms [9]. Several methods have been applied to the analysis of IgG N-glycans, such as reversed phase-high performance liquid chromatography (RP-HPLC), liquid chromatography-mass spectrometry (LC-MS), lectin analysis, and high pH anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD) [10].…”

Section: Introductionmentioning

confidence: 99%

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Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Nakagawa

Hato

Takegawa

et al. 2007

Journal of Chromatography B

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Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have been observed. To further analysis of N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with previous reports regarding IgG.We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC-MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Nakagawa

Hato

Takegawa

et al. 2007

Journal of Chromatography B

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“…Then CRP replaced ESR in the next developed models for predicting disease activity since it was a more reliable acute phase reactant and sensitive to short-term changes in clinical activity than ESR [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Serum adenosine deaminase may predict disease activity in rheumatoid arthritis

Zamani

Jamali

2011

Rheumatol Int

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To determine the relationship between serum adenosine deaminase (ADA) and disease activity, and to develop a new disease activity index based on serum ADA in rheumatoid arthritis (RA). Seventy RA patients were included. Disease activity based on Disease Activity Score 28-ESR (DAS28-ESR) and Disease Activity Score 28-CRP (DAS28-CRP) and serum ADA were measured. There were correlations when serum ADA compared with DAS28-ESR and DAS28-CRP. (R (2) = 0.014, 0.175, respectively, P values < 0.00). New disease activity index was developed by replacing ADA with ESR and CRP in DAS28-ESR and DAS28-CRP. There were strong correlations when new model compared with DAS28-ESR and DAS28-CRP. (R (2) = 0.94 and 0.95, respectively, P values < 0.00) The best new model values corresponding to DAS28-ESR values of 2.6, 3.2, and 5.1 were 2.79, 3.4, and 4.82, respectively; and new model values corresponding to DAS28-CRP values of 2.3, 2.7, and 4.1 were 2.1, 2.9, and 4, respectively. There were agreements when the new model compared with DAS28-ESR and DAS28-CRP for determination of patients in different disease activity categories. (Kappa = 0.81 and 0.71, respectively, P values < 0.00). The new disease activity index that applies serum ADA may help in predicting disease activity in RA.

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“…Several studies have shown that, in the etiology of RA, alterations in the glycosylation of plasma proteins are involved [4,10], including transferrin which consists of a polypeptide sequence of two oligosaccharide chains being N-glycosidally bound [2]. The association between CDT and RA was found by Sillanaukee et al [13].…”

Section: Discussionmentioning

confidence: 99%

“…Rheumatoid arthritis (RA) is a disease in which the glycosylation of plasma proteins is altered [4,10]. Evidence for these abnormalities was the presence of IgG isoforms having different numbers of galactose residues [11,15], due to reduced galactosyltransferase activity in lymphocyte B [9].…”

mentioning

confidence: 99%

Relationship between CDT and disease activity in rheumatoid arthritis

et al. 2012

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The aim of this study was to estimate the serum concentration of carbohydrate-deficient transferrin (CDT) in patients with rheumatoid arthritis (RA) and the relationship between the CDT level and disease activity in RA patients. Studies were carried out in 47 female patients with RA and 32 healthy women. Disease activity of RA was evaluated using the 28-joint count Disease Activity Score (DAS 28). Serum CDT was determined by particle-enhanced immunononephelometry using the N Latex CDT test. Patients with RA had significantly lower serum concentrations of CDT compared with controls. The correlation study showed the significant negative relationship between CDT and DAS 28 (r = - 0.483, p = 0.011). There were no correlations between serum CDT level and patient's age, disease duration, number of tender and swollen joints, and degree of disability evaluated by the Health Assessment Questionnaire. The level of CDT in patients with RA was significantly decreased and confirms the changes in transferrin glycosylation which are dependent on the disease activity. Therefore, measurement of CDT in the sera of patients with RA can be useful for the evaluation of disease activity in these patients.

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Altered expression and glycosylation of plasma proteins in rheumatoid arthritis

Cited by 27 publications

References 21 publications

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Serum adenosine deaminase may predict disease activity in rheumatoid arthritis

Relationship between CDT and disease activity in rheumatoid arthritis

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