Altered erythrocyte sodium‐lithium counter‐transport and Na+/K+‐ATPase activity in cystic fibrosis

Luczay, Andrea; Vásárhelyi, Barna; Dobos, M.; Holics, K; Újhelyi, Rita; Tulassay, Tivadar

doi:10.1111/j.1651-2227.1997.tb08883.x

Cited by 6 publications

(6 citation statements)

References 12 publications

(6 reference statements)

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“…It is difficult to speculate as to the implications of the inhibitory effect of tea catechins on Na + /K + ‐ATPase activity with ageing because there was no significant change in the activity of this enzyme in erythrocytes obtained from young and old humans. The finding that tea catechins may inhibit the activity of Na + /K + ‐ATPases may be beneficial in some age‐associated diseases in which enhanced Na + /K + ‐ATPase activity has been observed . The inhibitory effect of tea catechins on Na + /K + ‐ATPase activity may also be explained by the non‐specific effects of these polyphenols on membrane‐bound transporters.…”

Section: Discussionsupporting

confidence: 79%

“…The finding that tea catechins may inhibit the activity of Na + /K + -ATPases may be beneficial in some age-associated diseases in which enhanced Na + /K + -ATPase activity has been observed. 10,32,33 The inhibitory effect of tea catechins on Na + /K + -ATPase activity may also be explained by the non-specific effects of these polyphenols on membrane-bound transporters. Of the tea catechins, EGCG and ECG have been shown to affect membrane fluidity more significantly than EC and EGC.…”

Section: Discussionmentioning

confidence: 99%

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Erythrocyte membrane transporters during human ageing: Modulatory role of tea catechins

Pandey

Jha

Rizvi

2013

Clin Exp Pharma Physio

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Ageing is associated with many physiological and cellular changes, many of which are due to alterations in the plasma membrane. The functions of membrane transporter proteins are crucial for the maintenance of ionic homeostasis between the extra- and intracellular environments. The aim of the present study was to determine the status of erythrocyte membrane transporters, specifically Ca(2+) -ATPases, Na(+) /K(+) -ATPases and the Na(+) /H(+) exchanger (NHE), during ageing in humans. Furthermore, because tea catechins have been reported to possess strong anti-oxidant potential, the study was extended to evaluate the effect of (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC) and (-)-epigallocatechin-3-gallate (EGCG) on these transporters as a function of human age. The study was performed on 97 normal healthy subjects (62 men, 35 women; 16-80 years old). To investigate the effects of tea catechins, subjects were divided into three groups: young (<40 years old; n = 34); middle-aged (40-60 years old; n = 32); and old (>60 years old; n = 31). Erythrocyte ghosts/cell suspension from each group were incubated with ECG, EGCG, EGC and EC (10 μmol/L) for 30 min at 37°C prior to assay. Ageing significantly increased NHE activity and decreased Ca(2+) -ATPase activity. There were no significant changes in Na(+) /K(+) -ATPase activity during the ageing process. (-)-Epigallocatechin-3-gallate, EGC, ECG and EC effectively mitigated the changes in membrane transporter activity in erythrocytes from all age groups; however, the effect was more pronounced in the old age group. We hypothesize that impairment in -bound transporters may be one of the possible mechanisms underlying the pathological events during ageing. A higher intake of catechin-rich food may provide some protection against age-dependent diseases.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Erythrocyte membrane transporters during human ageing: Modulatory role of tea catechins

Pandey

Jha

Rizvi

2013

Clin Exp Pharma Physio

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“…To determine the functional results of the Na + /K + -pump, the intracellular Na + and K + concentrations were measured (12). The healthy reference range was the same as was reported by Luczay et al (12). To compare these results with those of healthy controls, two sample t tests were used.…”

Section: Methodsmentioning

confidence: 99%

Decreased Na+/K+ Pump Activity in the Erythrocytes of Children With Treated Celiac Disease

Vásárhelyi,

Dobos,

Bodánszky

et al. 2001

J. pediatr. gastroenterol. nutr.

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“…Abnormalities in red cell membrane essential fatty acid content and an increased susceptibility to oxidative damage have been recently described in CF patients, supporting the use of red cells as a cell model in CF [35]–[39]. Physiological studies on red cell membrane permeability in CF patients are contradictory and not conclusive, most likely related to the differences in the CF population studied [40]–[44].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evidence for Cell Membrane Amelioration in Children with Cystic Fibrosis by 5-MTHF and Vitamin B12 Supplementation: A Single Arm Trial

et al. 2009

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BackgroundCystic fibrosis (CF) is one of the most common fatal autosomal recessive disorders in the Caucasian population caused by mutations of gene for the cystic fibrosis transmembrane conductance regulator (CFTR). New experimental therapeutic strategies for CF propose a diet supplementation to affect the plasma membrane fluidity and to modulate amplified inflammatory response. The objective of this study was to evaluate the efficacy of 5-methyltetrahydrofolate (5-MTHF) and vitamin B12 supplementation for ameliorating cell plasma membrane features in pediatric patients with cystic fibrosis.Methodology and Principal FindingsA single arm trial was conducted from April 2004 to March 2006 in an Italian CF care centre. 31 children with CF aged from 3 to 8 years old were enrolled. Exclusion criteria were diabetes, chronic infections of the airways and regular antibiotics intake. Children with CF were supplemented for 24 weeks with 5-methyltetrahydrofolate (5-MTHF, 7.5 mg /day) and vitamin B12 (0.5 mg/day). Red blood cells (RBCs) were used to investigate plasma membrane, since RBCs share lipid, protein composition and organization with other cell types. We evaluated RBCs membrane lipid composition, membrane protein oxidative damage, cation content, cation transport pathways, plasma and RBCs folate levels and plasma homocysteine levels at baseline and after 24 weeks of 5-MTHF and vitamin B12 supplementation. In CF children, 5-MTHF and vitamin B12 supplementation (i) increased plasma and RBC folate levels; (ii) decreased plasma homocysteine levels; (iii) modified RBC membrane phospholipid fatty acid composition; (iv) increased RBC K+ content; (v) reduced RBC membrane oxidative damage and HSP70 membrane association.Conclusion and Significance5-MTHF and vitamin B12 supplementation might ameliorate RBC membrane features of children with CF.Trial RegistrationClinicalTrials.gov NCT00730509

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Altered erythrocyte sodium‐lithium counter‐transport and Na+/K+‐ATPase activity in cystic fibrosis

Cited by 6 publications

References 12 publications

Erythrocyte membrane transporters during human ageing: Modulatory role of tea catechins

Erythrocyte membrane transporters during human ageing: Modulatory role of tea catechins

Decreased Na+/K+ Pump Activity in the Erythrocytes of Children With Treated Celiac Disease

Preliminary Evidence for Cell Membrane Amelioration in Children with Cystic Fibrosis by 5-MTHF and Vitamin B12 Supplementation: A Single Arm Trial

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