2022
DOI: 10.1159/000526062
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Altered Endometrial Expression of α-Inhibin Subunit and Its Co-Receptor Betaglycan in Infertile Women with Endometriosis

Abstract: <b><i>Background:</i></b> Inhibins and their co-receptor betaglycan are members of the transforming growth factor β superfamily, a group of signaling molecules that control the differentiation of human endometrium in the secretory phase of the menstrual cycle. <b><i>Objective:</i></b> Since endometriosis is associated with endometrial dysfunction and infertility, this study aimed at evaluating the expression of α-inhibin and betaglycan mRNA and proteins in endome… Show more

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Cited by 4 publications
(2 citation statements)
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“…First‐strand complementary deoxyribonucleic acid (cDNA) was synthesized from 750 ng of DNase I‐treated total RNA using Superscript IV first‐strand synthesis system (CAT: 18091050; Invitrogen). Real‐time PCR was carried out as described previously (Dela Cruz et al, 2022) in an ABI‐Prism 7500 Sequence Detection System using the fluorescent dye Power SYBR Green Master Mix Kit (Invitrogen Life Technologies). The PCR parameters were: [stage 1] a cycle of 95°C/10 min; [stage 2] 40 cycles of 95°C/15 s, 60°C/15 s and 72°C/20 s; [stage 3] 95°C/15 s, 54°C/15 s and 95°C/15 s. The gene encoding the ribosomal protein S26 was used as the internal control.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…First‐strand complementary deoxyribonucleic acid (cDNA) was synthesized from 750 ng of DNase I‐treated total RNA using Superscript IV first‐strand synthesis system (CAT: 18091050; Invitrogen). Real‐time PCR was carried out as described previously (Dela Cruz et al, 2022) in an ABI‐Prism 7500 Sequence Detection System using the fluorescent dye Power SYBR Green Master Mix Kit (Invitrogen Life Technologies). The PCR parameters were: [stage 1] a cycle of 95°C/10 min; [stage 2] 40 cycles of 95°C/15 s, 60°C/15 s and 72°C/20 s; [stage 3] 95°C/15 s, 54°C/15 s and 95°C/15 s. The gene encoding the ribosomal protein S26 was used as the internal control.…”
Section: Methodsmentioning
confidence: 99%
“…). Real-time PCR was carried out as described previously(Dela Cruz et al, 2022) in an ABI-Prism 7500 Sequence Detection…”
mentioning
confidence: 99%