Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature

Rodrı́guez, Dolores; Nicolás, G.; Aldasoro, Juan José; Hernández-Nistal, Josefina; Babiano, M. J.; Matilla, Ángel J.

doi:10.1007/bf00395969

Cited by 18 publications

(10 citation statements)

References 28 publications

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“…When stressed in the absence of ABA, the cells began to synthesize 26-kDa protein only on day 32 (Fig. 2B, 14 (Fig. 4A, lanes 1-4, respectively).…”

Section: Resultsmentioning

confidence: 99%

“…Barley aleurone cells have been used extensively to study ABA inhibition of giberellic acid-induced synthesis of aamylase, where ABA appears to affect the level of transcription and transcript stability and processing (4,5,(18)(19)(20). In other tissues, ABA appears to act at the level of translation (14,17) or transcription (15,16).…”

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confidence: 99%

“…ABA generally exerts an inhibitory effect on the metabolism of nucleic acids and proteins. Yet, it has been shown to induce the synthesis of specific mRNAs and proteins, notably in barley aleurohe layers (4)(5)(6)(7) and in other plants during seed germination and embryogenesis (8)(9)(10)(11)(12)(13)(14), desiccation and other stresses (15,16), and dormancy (17). Barley aleurone cells have been used extensively to study ABA inhibition of giberellic acid-induced synthesis of aamylase, where ABA appears to affect the level of transcription and transcript stability and processing (4,5,(18)(19)(20).…”

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confidence: 99%

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Hormonal regulation of protein synthesis associated with salt tolerance in plant cells

Singh

LaRosa

Handa

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

144

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Cultured tobacco cells (Nicotiana tabacum L. cv. Wisconsin 38) synthesize a predominant 26-kDa protein upon exposure to abscisic acid (ABA). ABA also accelerates the rate of adaptation of unadapted cells to NaCl stress. The ABA-induced 26-kDa protein is immunologically cross-reactive to, and produces a similar pattern of peptides after partial proteolysis as, the major 26-kDa protein associated with NaCl adaptation. Both Abscisic acid (ABA) has been implicated as having an important role in many plant physiological processes (1-3). However, molecular events mediated by ABA are poorly understood. ABA generally exerts an inhibitory effect on the metabolism of nucleic acids and proteins. Yet, it has been shown to induce the synthesis of specific mRNAs and proteins, notably in barley aleurohe layers (4-7) and in other plants during seed germination and embryogenesis (8-14), desiccation and other stresses (15, 16), and dormancy (17). Barley aleurone cells have been used extensively to study ABA inhibition of giberellic acid-induced synthesis of aamylase, where ABA appears to affect the level of transcription and transcript stability and processing (4,5,(18)(19)(20). In other tissues, ABA appears to act at the level of translation (14, 17) or transcription (15,16).We have demonstrated that tobacco cells undergo phenotypic and physiological changes during exposure to and subsequent adaptation to NaCl and accumulate several proteins (21). In addition, we have shown that ABA accelerates the rate of adaptation to NaCl (22). In this report, we present evidence of the involvement of ABA in the synthesis of a 26-kDa protein which is associated with NaCl adaptation. We also show that the ABA-induced 26-kDa proteins from cultured cells of several plant species are immunologically related and that there is tissue-specific expression of the 26-kDa protein in differentiated tissues of tobacco plants. (pH 7.0) containing 0.5 M NaCl, 0.1% NaDodSO4, 0.5% Triton X-100, and 1 mM phenylmethylsulfonyl fluoride] and placed in boiling water for 2 min. A clear supernatant was obtained by centrifugation at 12,000 x g for 5 min. 35S incorporation into protein was measured after trichloroacetic acid precipitation as described (21). Twenty microliters of partially purified (IgG fraction) rabbit antiserum against 26-kDa protein from S-25 cells was used to immunoprecipitate 35S-labeled protein (equal cpm) in 500 ,ul of clear supernatant by incubation at 4°C overnight. Antibody-antigen complex was precipitated by addition of protein A attached to Staphylococcus aureus wall as described by Kessler (24). The immunoprecipitated protein complex was dissociated in extraction buffer containing 65 mM Tris HCl (pH 6.8), 2% NaDodSO4, 5% (vol/vol) glycerol, 5% 2-mercaptoethanol, 2 mM EDTA, and 1 mM phenylmethylsulfonyl fluoride by incubation in boiling water followed by centrifugation at 12,000 x g for 2 min. Conditions of single and two-dimensional electrophoresis, partial proteolysis, staining, and fluorography of gels were as described (21)....

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“…When stressed in the absence of ABA, the cells began to synthesize 26-kDa protein only on day 32 (Fig. 2B, 14 (Fig. 4A, lanes 1-4, respectively).…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Hormonal regulation of protein synthesis associated with salt tolerance in plant cells

Singh

LaRosa

Handa

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

144

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“…To isolate polysomal RNA from leaf tissue, several protocols were combined, modified and optimized for use with Arabidopsis (mainly according to Jackson and Larkins 1976;Rodriguez et al 1985; also Leaver and Dyer 1974;Larkins 1985). Extraction conditions were set in order to achieve the isolation of both free and membrane-bound polysomes.…”

Section: Methodsmentioning

confidence: 99%

A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Lechner

Rau

1993

Planta

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Abstract. Previous studies have indicated that the photoperiodic induction of flowering results in qualitative changes in gene expression in the leaves of the induced plant. However, we obtained different results when leaf tissue was examined at different sampling times after flower induction, thus pointing to the possibility that alterations in gene expression during photoperiodic induction are only transient. We analyzed the composition of mRNA populations in the leaves of the long-day plant Arabidopsis thaliana (L.) Heynh. at various times during photoperiodic induction. Polysomal mRNA populations were assayed during illumination programs consisting of different photoperiodic cycles. Quantitative changes of distinct leaf mRNAs were observed during the time course. The expression levels of most of these mRNAs oscillated in a diurnal or circadian rhythm with respect to the photoperiod. However, no novel mRNA appeared or decreased to undetectable levels during the changes in photoperiod. After the onset of inducing photoperiods, alterations in the phase and amplitude of these oscillations were seen. Various types of oscillation pattern were observed. These changes occurred either very rapidly, within hours after the additional light period began or appeared after the first altered photoperiod was complete. The majority of the changes seen at the beginning of inductive photoperiods were transient and the mRNA levels reverted to the original pattern when the plant was returned to non-inductive photoperiods. In addition, some of these alterations were found to be transient, irrespective of the length of subsequent light periods.

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“…At 30°C and above the emergence of the radicle is delayed and the capacity for ion exchange in the embryonic axis (1 1), genetic expression (26), and cytokinin levels (25) are all affected. At 25°C ethylene production is related to cell growth (28).…”

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Ethylene Production and 1-Aminocyclopropane-1-Carboxylic Acid Conjugation in Thermoinhibited Cicer arietinum L. Seeds

Gallardo¹,

Delgado²,

Sánchez‐Calle³

et al. 1991

Plant Physiol.

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The effect of supraoptimal temperatures (300C, 350C) on germination and ethylene production of Cicer arietinum (chick-pea) seeds was measured. Compared with a 250C control, these temperatures inhibited both germination and ethylene production. The effect of supraoptimal temperatures could be alleviated by treating the seeds with ethylene. It was concluded that one effect of high temperature on germination was due to its negative effect on ethylene production. This inhibitory effect of high temperature was due to increased conjugation of 1-aminocyclopropane-1-carboxylic acid to 1-(malonylamino)cyclopropane-1-carboxylic acid and to an inhibition of ethylene-forming enzyme activity.Seeds germinate at a certain optimum temperature, above which germination may be delayed or inhibited, a phenomenon known as thermoinhibition (1). This can be overcome by various phytohormones, one of which is ethylene, and indeed small quantities of this phytohormone are produced in some thermoinhibited seeds (1, 18).The optimum temperature for the germination of Cicer arietinum seeds is 25°C. At 30°C and above the emergence of the radicle is delayed and the capacity for ion exchange in the embryonic axis (1 1), genetic expression (26), and cytokinin levels (25) are all affected. At 25°C ethylene production is related to cell growth (28). In other tissues high temperatures appear to inhibit the conversion of ACC2 into ethylene rather than interfere with the SAM to ACC step (8).In plants ethylene is synthesized from methionine via adenosyl-methionine and ACC (30). The conversion of SAM or adenosyl-methionine into ACC is catalyzed in the cytosol by ACC-synthase and is the principal point ofcontrol for biosynthesis ofethylene (29). The mechanism for the transformation of ACC into ethylene is less well understood. However, EFE activity has been associated with the vacuole membranes (10) and other compartments of the cell (15).ACC is also metabolized into MACC in the cytosol (4 (14), in some tissues the possibility exists that MACC is converted into ACC via MACC-acylase. MATERIALS AND METHODS Vegetable Materials and Seed GerminationSeeds of Cicer arietinum cv Castellana (chick-pea) were bought commercially and stored at 4°C until required.The seeds were washed in sterile, double-distilled water and incubated in batches of 50 at 25°C, 30°C or 35C, for a minimum of 6 h and a maximum of 36 h, in plastic trays containing 175 mL sterile distilled water. The percentage of germination was measured for all the times and temperatures, taking the emergence of the radicle as the definition of germination.The embryonic axes and cotyledons were aseptically removed after incubation to measure ethylene production, levels of free and conjugated ACC, and activity of ACC-synthase and EFE. Ethylene MeasurementsTwenty five (500 mg) isolated embryonic axes and 10 (4.5 g) cotyledons were aseptically transferred to 50 mL flasks containing 0.5 mL distilled water. The flasks were sealed with silicone-rubber stoppers and incubated in darkness at 25°C. After ...

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Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature

Cited by 18 publications

References 28 publications

Hormonal regulation of protein synthesis associated with salt tolerance in plant cells

Hormonal regulation of protein synthesis associated with salt tolerance in plant cells

A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Ethylene Production and 1-Aminocyclopropane-1-Carboxylic Acid Conjugation in Thermoinhibited Cicer arietinum L. Seeds

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