Altered cytoskeletal structures in transformed cells exhibiting obviously metastatic capabilities

Abstract

Cytoskeletal changes in transformed cells (LM-51) exhibiting obviously metastatic capabilities were investigated by utilization of double-fluorescent labelling through combinations of: (1) tubulin indirect immunofluoreseonce plus Rhodamine-phalloidin staining of F-actins; (2) indirect immunofluorescent staining with α-actinin polyclonal-and vinculin monoclonal antibodies. The LM-51 cells which showed metastatic index of >50% were derived from lung metastasis in nude mice after subcutaneous inoculation of human highly metastatic tumor DNA transfected NIH3T3 cell transformants. The parent NIH3T3 cells exhibited well-organized microtubules, prominent stress fibers and adhesion plaques while their transformants showed remarkable eytoskeletal alterations: (l) reduced microtubules but increased MTOC fluorescence; (2) disrupted stress fibers and fewer adheaion plaques with their protein components redistributed in the cytoplasm; (3) Factin-and α-actinin/vinculin aggregates appeared in the cytoplasm. These aggregates were dot-like, varied in size (0.1− 0.4μm) and number, located near the ventral surface of the cells. TPA-induced actin/vineulin bodies were studied too. Indications that aotin and α-actinin/vinoulin redistribution might be important alterations involved in the expression of metastatic capabilities of LM-51 transformed cells were discussed.

Mechanical phenotyping of cells and extracellular matrix as grade and stage markers of lung tumor tissues

“…In comparison with healthy cells, biomechanical investigations reported some common features of many types of tumor cell lines, such as a less structured CSK [7][8][9][10] with lower cell mechanical [10][11][12] and cyto-adhesive properties [9,10,13,14]. All factors which, from a biophysical point of view, augment the metastatic potential of the cancer cells [3,7,9,14,15].…”

Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium

“…These include immortalization of retroviral transduction by simian virus 40 (SV40) T antigen [ 37 , 38 ], Cdk4R24c/CyclinD1 [ 39 ], and/or human papilloma virus 16 E6/E7 [ 40 ]. Immortalized cells increase the risk of tumor formation, aneuploidy [ 41 ], and structural rearrangements [ 42 ]. Recently, the establishment of untransfected HCEC line [ 43 ] and immortalization of HCECs with human telomerase reverse transcriptase have been explored [ 44 ].…”

Differential response of MDA‑MB‑231 breast cancer and MCF10A normal breast cells to cytoskeletal disruption

“…The cytoskeleton is a dynamic network of filamentous proteins that controls cell shape, maintains intracellular organization and is involved in cell motility ( 1 , 2 ). Alterations in cytoskeletal structures serve a pivotal role in controlling cancer cell behavior, including cell adhesion, migration and invasion ( 3 , 4 ).…”