Altered content composition and structure of glycosaminoglycans and proteoglycans in gastric carcinoma

Theocharis, Achilleas D.; Vynios, Demitrios H.; Papageorgakopoulou, N.; Skandalis, Spyros S.; Theocharis, Dimitrios A.

doi:10.1016/s1357-2725(02)00264-9

Cited by 126 publications

(129 citation statements)

References 48 publications

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“…Heightened levels of both hyaluronan and versican in tumor progression have been reported previously (45,46,64). It has also been reported that in many cancers the level of hyaluronan is considered to be a reliable prognostic indicator of malignant progression (65).…”

Section: Discussionmentioning

confidence: 81%

A Role for Versican in the Development of Leiomyosarcoma

Keire

Bressler

Lemire

et al. 2014

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 81%

A Role for Versican in the Development of Leiomyosarcoma

Keire

Bressler

Lemire

et al. 2014

Journal of Biological Chemistry

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“…DDi-0S disaccharide is the predominant disaccharide found in pancreatic carcinoma (10) and is increased 4-fold in gastric carcinoma compared with normal tissue (28). Furthermore, gastric carcinomas exhibit increased DDi-0S and DDi-6S disaccharides with a parallel decrease in DDi-4S disaccharides.…”

Section: Cancer Epidemiology Biomarkers and Prevention Cancer Epidemiomentioning

confidence: 96%

Immunohistochemical Level of Unsulfated Chondroitin Disaccharides in the Cancer Stroma Is an Independent Predictor of Prostate Cancer Relapse

Sakko

Butler

Byers

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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The glycosaminoglycan chondroitin sulfate is significantly increased in the peritumoral stroma of prostate tumors compared with normal stroma and is an independent predictor of prostate-specific antigen (PSA) relapse following radical prostatectomy. In this study, we determined whether specific alterations in the sulfation pattern of glycosaminoglycan chains in clinically organ-confined prostate cancer are associated with PSA relapse. Immunoreactivity to distinct glycosaminoglycan disaccharide epitopes was assessed by manually scoring the staining intensity in prostate tissues from patients with benign prostatic hyperplasia (n = 19), early-stage cancer (cohort 1, n = 55 and cohort 2, n = 275), and advanced-stage cancer (n = 20). Alterations to glycosaminoglycans in benign and malignant prostate tissues were determined by cellulose acetate chromatography and high-pressure liquid chromatography. Glycosaminoglycan disaccharide epitopes were localized to the peritumoral stroma of clinically localized prostate cancer. The level of immunostaining for unsulfated disaccharides (C0S) in the peritumoral stroma, but not for 4-sulfated (C4S) or 6-sulfated disaccharides (C6S), was significantly associated with the rate of PSA relapse following radical prostatectomy. High levels of C0S immunostaining were determined to be an independent predictor of PSA relapse (1.6-fold, P = 0.020). Advanced-stage prostate cancer tissues exhibited reduced electrophoretic mobility for chondroitin sulfate and increased unsulfated disaccharides when compared with benign prostatic hyperplasia tissues, whereas the sulfated disaccharide levels were unaffected. The level of C0S immunostaining in the peritumoral stroma is an independent determinant of PSA failure in clinically localized prostate cancer. Specific alterations to chondroitin sulfate side chains occurring during tumor development may be a crucial step for disease progression in prostate cancer. (Cancer Epidemiol Biomarkers Prev 2008;17(9):2488 -97)

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“…Asporin (ASPN), a novel member of the small leucine-rich repeat proteoglycan (SLRP) family, contains a unique aspartate-rich N terminus and is involved in the stromal modification of ECM (6). The expression levels of ASPN and other related matrix proteoglycans, for instance decorin and biglycan, have been assessed in gastric pathology, and are implicated in the oncogenesis and development of human gastric carcinoma (7)(8)(9)(10). Particularly, Satoyoshi et al recently concluded that ASPN activates the coordinated invasion of scirrhous gastric cancer and cancer-associated fibroblasts by activation of the CD44-Rac1 pathway (7).…”

Section: Introductionmentioning

confidence: 99%

Asporin participates in gastric cancer cell growth and migration by influencing EGF receptor signaling

2015

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Abstract. Asporin (ASPN), a novel member of the small leucine-rich proteoglycan (SLRP) family, serves as a key component of the tumor stroma and has been reported to be abnormally expressed in certain types of tumors. Specifically, the proteoglycan was proven to activate the coordinated invasion of scirrhous gastric cancer and cancer-associated fibroblasts. However, the role of ASPN in cancer cell growth and metastasis has not yet been addressed. In the present study, we aimed to evaluate the tumoricidal benefits of ASPN on tumorigenesis and progression of gastric cancer. Firstly, it was demonstrated that ASPN was overexpressed in gastric carcinoma tissues when compared to the corresponding non-cancerous tissues, and it had varied levels of expression in gastric cancer epithelial cell lines. Additionally, we assessed the effects of transient siRNA-mediated ASPN knockdown on gastric cancer cells. ASPN silencing inhibited proliferation and suppressed the migration of immortalized neoplastic epithelial cells. Furthermore, at the molecular level, we found that downregulation of ASPN blocked the anti-apoptotic molecule Bcl-2, increased the expression of pro-apoptotic molecule Bad, reduced the expression of migration-related proteins CD44 and matrix metalloproteinase (MMP)-2, and abrogated the activation of the phosphorylation status of ERK and epidermal growth factor (EGF) and its receptor (EGFR). Collectively, our findings indicate that ASPN is upregulated and plays an oncogenic role in gastric cancer progression and metastasis by influencing the EGFR signaling pathway.

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Altered content composition and structure of glycosaminoglycans and proteoglycans in gastric carcinoma

Cited by 126 publications

References 48 publications

A Role for Versican in the Development of Leiomyosarcoma

A Role for Versican in the Development of Leiomyosarcoma

Immunohistochemical Level of Unsulfated Chondroitin Disaccharides in the Cancer Stroma Is an Independent Predictor of Prostate Cancer Relapse

Asporin participates in gastric cancer cell growth and migration by influencing EGF receptor signaling

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