Altered Composition of Microbiota in Women with Ovarian Endometrioma: Microbiome Analyses of Extracellular Vesicles in the Peritoneal Fluid

Lee, Sa Ra; Lee, Jae Chul; Kim, Sung Hoon; Oh, Young-Sang; Chae, Hee Dong; Seo, Ho-Chan; Kang, Chil-Sung; Shin, Tae-Seop

doi:10.3390/ijms22094608

Cited by 26 publications

(59 citation statements)

References 34 publications

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“…After excluding 65 duplicates, 122 of the remaining 144 studies were excluded following title and abstract review. Full-text screening of 23 studies to evaluate for inclusion and exclusion criteria according to the study design, type of publication, methods, and results was performed by two authors (CHM and FOR), yielding 12 articles, all case–control studies ( Khan et al 2016 , Xu et al 2017 , Wang et al 2018 , Akiyama et al 2019 , Ata et al 2019 , Chen et al 2020 , Hernandes et al 2020 , Perrotta et al 2020 , Wei et al 2020 , Chao et al 2021 , Lee et al 2021 , Svensson et al 2021 ), meeting study inclusion criteria for data abstraction and qualitative analysis ( Fig. 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Two Next Generation Sequencing (NGS) techniques were used to evaluate microbiomes: Microbiome Shotgun Sequencing (Chao et al, 2021;Ata et al, 2019;Chen et al, 2020;Hernandes et al, 2020;Khan et al, 2016;Lee et al, 2021;Perrotta et al, 2020;Svensson et al, 2021;Wang, Ma and Song, 2018;Xu et al, 2017) and Ion Torrent Personal Genome Machine (Akiyama et al, 2019;Wei et al, 2020). Akiyama et al, 2019, also used real-time polymerase chain reaction (PCR) for quantification of Enterobacteriaceae, Streptococcus, Pseudomonas, and Corynebacterium genus.…”

Section: Methods Of Evaluation Of Microbiomementioning

confidence: 99%

“…RNA gene (16s-rRNA), including: V1-V3 (Svensson et al, 2021), V3-V4 (Ata et al, 2019;Chen et al, 2020;Hernandes et al, 2020;Lee et al, 2021;Xu et al, 2017), V4 (Chao et al, 2021;Ata et al, 2019;Wei et al, 2020;Hernandes et al, 2020;Lee et al, 2021;Xu et al, 2017;Wang, Ma and Song, 2018;Khan et al, 2021;Khan et al, 2016;Chao et al, 2021;Perrotta et al, 2020;Svensson et al, 2021;Chen et al, 2020). One study (Khan et al, 2016) did not specify the rRNA amplification region.…”

Section: Studies Using Ngs Techniques Analyzed Different Amplified Re...mentioning

confidence: 99%

“…Three studies (Lee et al, 2021;Wang, Ma and Song, 2018;Wei et al, 2020) analyzed the relationship between endometriosis and the microbiome within peritoneal fluid, one of which (Wei et al, 2020) also collected samples from other sites. Lee et al, 2021, compared 45 women with stages III and IV endometriosis (mean age 36.2 ± 1.3 years old) against 45 controls who underwent laparoscopy, 31 for myomas and 14 for benign ovarian cysts (mean age 39.4 ± 1.1 years old).…”

Section: Peritoneal Fluid Microbiomementioning

confidence: 99%

“…Nonetheless several trends appear to stand out from these imperfect, heterogeneous studies. Several studies suggest that peritoneal fluid appears to contain a different distribution of bacteria among women with endometriosis, though only Pseudomonas (Lee et al, 2021;Wei et al, 2020) was found to be overrepresented among patients with endometriosis in multiple studies.…”

Section: Endometriosis Symptoms and Microbiomementioning

confidence: 99%

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The microbiome and endometriosis

Miyashira

Oliveira

Andres

et al. 2022

Reproduction and Fertility

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The objective of this study was to systematically review the literature on the human microbiome in association with endometriosis. PubMed/Medline, Cochrane, and Embase databases were searched for literature published from 1986 to August 2021. All human studies that assessed the microbiome using 16S rRNA sequencing or by Shotgun Sequence in women with endometriosis were included. Two reviewers independently abstracted data from the selected articles into tables. To assess the quality of included studies, NIH Study Quality Assessment Tools were utilized. This review included 12 case-control studies. Included studies compared the microbiome from various anatomical sources (fecal, vaginal, cervical, peritoneal, endometrial, intra-lesional) between patients with endometriosis and a heterogeneous set of control patients. Study quality ranged from poor to good, with 8 of 12 studies rated fair. Multiple studies reported on a different distribution of bacteria among women with endometriosis across anatomical sites, but results were highly heterogeneous. Pseudomonas was overrepresented in peritoneal fluid among women with endometriosis across multiple studies, but was also observed increased in vaginal, endometrial, and intra-lesional samples. Among bacteria noted across different anatomical samples, Gardnerella was increased in cervical but decreased in endometrial, fecal, and vaginal samples of patients with endometriosis, while Atopium was decreased in vaginal and cervical samples from patients with endometriosis. Sphingobium was increased in vagina, endometrium, and peritoneal fluid from patients with endometriosis. Streptococcus was increased peritoneal, endometrial, and cervical samples from women with endometriosis. Microbiomal comparisons stratified by endometriosis stage or site of endometriosis involvement were limited and highly heterogeneous.

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Section: Resultsmentioning

confidence: 99%

Section: Methods Of Evaluation Of Microbiomementioning

confidence: 99%

Section: Studies Using Ngs Techniques Analyzed Different Amplified Re...mentioning

confidence: 99%

Section: Peritoneal Fluid Microbiomementioning

confidence: 99%

Section: Endometriosis Symptoms and Microbiomementioning

confidence: 99%

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The microbiome and endometriosis

Miyashira

Oliveira

Andres

et al. 2022

Reproduction and Fertility

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Comparison of cervicovaginal fluid extracellular vesicles isolated from paired cervical brushes and vaginal swabs

Paterson,

Scheck,

McDowell

et al. 2024

J of Extracellular Bio

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Endometriosis is a common gynaecological condition, with a long diagnostic delay. Surgery is required to confirm a diagnosis, highlighting the need for a non‐invasive biomarker. Extracellular vesicles (EVs) may have a role in endometriosis pathogenesis, yet there is limited EV biomarker literature available. This study aimed to investigate the feasibility of isolating cervico‐vaginal fluid EVs sampled using cervical brushes and vaginal swabs and to compare these methods. After providing informed consent, patients undergoing surgery for suspected endometriosis had cervical brush and vaginal swab samples collected under general anaesthetic. Isolated EVs were characterised through negative stain transmission electron microscopy (TEM), Western blotting (TSG101, CD63, Calnexin, ApoB, Albumin), tunable resistive pulse sensing (TRPS), microBCA assays and RT‐qPCR of miRNAs. PCR was performed on samples prior to EV isolation to assess bacteria present in samples. Cervical brush and vaginal swab EVs were intact vesicles with limited co‐isolated contaminants. Cervical brushes had higher concentrations of particles compared to match vaginal swabs, although both samples had low concentrations. Protein and miRNA yield were similar between matched samples. PCR demonstrated only a small amount DNA within samples was bacterial (>0.5%). Cervico‐vaginal fluids EVs were successfully isolated from cervical brushes and vaginal swabs, demonstrating a new method of sampling reproductive EVs. EV yield from both sample types was low. Similar protein and miRNA levels suggest either sampling method may be suitable for biomarker studies.

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Diet in Prevention and Treatment of Endometriosis: Current State of Knowledge

Oszajca,

Adamus

2024

Curr Nutr Rep

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Altered Composition of Microbiota in Women with Ovarian Endometrioma: Microbiome Analyses of Extracellular Vesicles in the Peritoneal Fluid

Cited by 26 publications

References 34 publications

The microbiome and endometriosis

The microbiome and endometriosis

Comparison of cervicovaginal fluid extracellular vesicles isolated from paired cervical brushes and vaginal swabs

Diet in Prevention and Treatment of Endometriosis: Current State of Knowledge

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