Altered Circulating Inflammatory Cytokines Are Associated with Anovulatory Polycystic Ovary Syndrome (PCOS) Women Resistant to Clomiphene Citrate Treatment

Wang, Lian Lian; Qi, Hong; Baker, Philip N.; Zhen, Qian Na; Zeng, Qian; Shi, Rui; Tong, Chao; Ge, Qian

doi:10.12659/msm.901194

Cited by 33 publications

(21 citation statements)

References 27 publications

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“…By using a cytokine antibody arrange confirmed by ELISA, one of the differences found was the circulating ANGPT2 levels, since PCOS women resistant to CC presented lower levels of ANGPT2 than sensitive ones. The authors conclude that CC-resistant women had an excessive ovarian angiogenesis that compromise their responsiveness to ovulation induction (Wang et al 2017).…”

Section: Angiopoietins (Angpts)mentioning

confidence: 96%

“…A recently published study examined the differences between women with PCOS that ovulated after clomiphene citrate (CC) and PCOS women resistant to CC (Wang et al 2017). By using a cytokine antibody arrange confirmed by ELISA, one of the differences found was the circulating ANGPT2 levels, since PCOS women resistant to CC presented lower levels of ANGPT2 than sensitive ones.…”

Section: Angiopoietins (Angpts)mentioning

confidence: 99%

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Ovarian angiogenesis in polycystic ovary syndrome

Pietro¹,

Pascuali²,

Parborell³

et al. 2018

Reproduction

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Polycystic ovary syndrome (PCOS) is the most prevalent endocrine pathology among women in reproductive age. Its main symptoms are oligo or amenorrhea, hyperandrogenism and the presence of ovarian cysts. It is also associated with infertility, obesity and insulin resistance. Mainly due to its heterogeneity, PCOS treatments are directed to manage its symptoms and to prevent associated diseases. The correct formation and regression of blood vessels during each ovarian cycle is indispensable for proper follicular development, ovulation and corpus luteum formation. The importance of these processes opened a new and promising field: ovarian angiogenesis. Vascular alterations characterize numerous pathologies, either with increased, decreased or abnormal angiogenesis. In the last years, several anomalies of ovarian angiogenesis have been described in women with PCOS. Therefore, it has been suggested that these alterations may be associated with the decreased - or lack of - ovulation rates and for the formation of cysts in the PCOS ovaries. Restoration of a proper vessel formation in the ovaries may lead to improved follicular development and ovulation in these patients. In the present review, we attempt to summarize the alterations in ovarian angiogenesis that have been described in women with PCOS. We also discuss the therapeutic approaches aimed to correct these alterations and their beneficial effects on the treatment of infertility in PCOS.

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Section: Angiopoietins (Angpts)mentioning

confidence: 96%

Section: Angiopoietins (Angpts)mentioning

confidence: 99%

Ovarian angiogenesis in polycystic ovary syndrome

Pietro¹,

Pascuali²,

Parborell³

et al. 2018

Reproduction

View full text Add to dashboard Cite

show abstract

“…PCOS is the main cause of anovulatory infertility, which affects 80% of patients with anovulatory infertility [12,13]. At present, the mechanism of ovarian injury and abnormal follicular development in PCOS patients is not fully understood.…”

Section: Discussionmentioning

confidence: 99%

Silencing Circ_0058063 Enhances Proliferation and Inhibits Apoptosis in PCOS Ovarian Granulosa Cells

Wen

Zhang

2020

Preprint

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Background Polycystic ovary syndrome (PCOS) is the most common endocrine disease in reproductive-aged women. This study was designed to explore the role of circ_0058063 in PCOS. Methods We recruited 25 PCOS patients and 25 no-PCOS patients. The concentrations of follicle stimulating hormone (FSH), testosterone (T), luteinizing hormone (LH), progesterone (P4) and estradiol (E2) were measured by radioimmunoassay. The level of aromatase was detected using an ELISA kit. The proliferation and apoptosis of ovarian granulosa cells were assessed using CCK-8 assay and flow cytometry, respectively. Gene and protein expression were evaluated through RT-qPCR and Western blotting assay. Results The circ_0058063 level in ovarian granulosa cells and follicular fluid is significantly higher in the PCOS group than the no-PCOS group. Besides, silencing circ_0058063 increases the levels of Aromatase mRNA, P4 and E2 in PCOS ovarian granulosa cells. Additionally, silencing circ_0058063 can promote the proliferation of ovarian granulosa cells in patients with PCOS. Furthermore, silencing circ_0058063 can suppress apoptosis of PCOS ovarian granulosa cells.Conclusions Silencing circ_0058063 enhances proliferation and inhibits apoptosis in PCOS ovarian granulosa cells. Such findings may offer vital insights into a therapeutic target for PCOS.

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“…OHS using gonadotropins is believed to induce inflammatory reaction leading to massive extravasation of fluids, inflammatory mediators, enlarging ovary and release of other proteins at varying levels [23]. Inflammatory reaction phase is followed by anti-inflammatory mechanism during maturation of follicles [24]. Whereas, ovarian cysts developed in PCOS patients are the structures with thin granulosa cells [25].…”

Section: Discussionmentioning

confidence: 99%

Role of Cystic Fluid Growth Factors and Inflammatory Mediators in Pathogenesis of Polycystic Ovary Syndrome

2019

JGRM

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Objective: Present study was carried out to investigate whether there is any localized growth factor/s involved and mediated by inflammatory cytokines through measuring their level in fluid aspirated from ovarian cyst. Study Design: Data was collected from 62 infertile female patients having PCOS and without PCOS. The cystic and follicular fluid obtained from consented patients was used in estimating inflammatory and growth factors. Further, these values were analyzed and interpreted with respect to number, size, length and depth of cysts. Results: Indicates that there is a low level of inflammatory reaction centralized to IL-18 triggering the release of MMP-2 to degrade the extracellular matrix followed by release of ANG-1, VEGF, IGF-1 and EGF to maintain the growth of ovarian cysts. Our findings support the prominent role of IL-18, MMP-2 and ANG-1 in pathogenesis of severe PCOS. Conclusion: In conclusion, the levels of inflammatory mediators in follicular fluid are significantly higher in women having PCOS than compared to cystic fluid of Non-PCOS patients. Despite the promising results of present study we recommend the readers to consider these results as preliminary. Further larger cohort studies are recommended by including more number of heterogeneous population, for extended period of time and more number of cytokines and growth factors with higher accuracy to understand how local inflammatory and growth factors are inter-related in development of ovarian cysts in PCOS patients.

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Altered Circulating Inflammatory Cytokines Are Associated with Anovulatory Polycystic Ovary Syndrome (PCOS) Women Resistant to Clomiphene Citrate Treatment

Cited by 33 publications

References 27 publications

Ovarian angiogenesis in polycystic ovary syndrome

Ovarian angiogenesis in polycystic ovary syndrome

Silencing Circ_0058063 Enhances Proliferation and Inhibits Apoptosis in PCOS Ovarian Granulosa Cells

Role of Cystic Fluid Growth Factors and Inflammatory Mediators in Pathogenesis of Polycystic Ovary Syndrome

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