Altered circular RNA expression profiles in an ovalbumin-induced murine model of allergic rhinitis

Chen, Jie; Xiao, Xiyan; He, Shan; Qiao, Yanjiang; Ma, Shuwei

doi:10.15586/aei.v49i2.33

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…For the mechanism, circRNAs performed critical functions maybe through endocytosis, cell adhesion molecules, and lipid metabolism contributed to allergic asthma [34].In addition to its value in the field of diagnosis and treatment of allergic disorders, circRNA as biomarkers which is important in identifying certain diseases for heterogeneity and potential relationship, ciRS-7 and circZRANB1 were showed as promising biomarkers for discriminating atopic dermatitis from psoriasis [35]. But in AR, circRNAs were reported to be involved in the pathogenesis of AR, and their potential role was further highlighted, at the level of AR murine models; the aberrantly expressed circRNAs were considered correlated with miRNAs that are involved in T-cell polarization and activation [16]. Moreover, circRNA arresting domaincontaining 3 contributed to the development of AR by regulating the miR-375/KLF4 axis; highly expressed circHIPK3 and lncGAS5 promoted Th2 differentiation of ovalbumin-induced CD4 + T cells and led to the aggravated nasal symptoms of AR mice that were revealed [36].…”

Section: Discussionmentioning

confidence: 99%

“…circRNA expression profiles in the nasal mucosa in patients with AR have been identified; of the 11 differentially expressed circRNAs that were screened by high-throughput sequencing, hsa_circ_0008668 and circTRIQK were up-regulated and hsa_circ_0029853 and circRNA_01002 were down-regulated in AR tissues [15]. Also, in nasal mucosa from ovalbumin-induced AR murine models, 86 aberrantly expressed circRNAs of which 51 were up-regulated and 35 were down-regulated were showed [16]. However, the study of the expression profile of circRNAs in peripheral blood of AR patients was absent.…”

Section: Introductionmentioning

confidence: 99%

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Peripheral Circular RNA Profiling from Patients with Allergic Rhinitis Identified hsa_circRNA_404013 as a Potential Diagnostic Biomarker

Peng

Sun

Zhang

et al. 2022

Int Arch Allergy Immunol

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Introduction: The study of peripheral circular RNA (circRNA) expression profile in patients with allergic rhinitis (AR) was absent to date, and we aimed to obtain the circRNA expression profile and identify the candidate biomarker from AR. Methods: circRNA chip was performed to screen differentially expressed circRNAs in the peripheral blood sample from AR patients and healthy controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways were analyzed to further search the function of the differential expressed circRNAs. The real-time quantitative reverse transcription-polymerase chain reaction was further used to verify the candidate circRNA and also analyze its potential correlation with clinical parameters. Results: Significantly up-regulated expression level of hsa_circRNA_404013 in AR was obtained by circRNA chip and further verified in 79 AR patients and 48 healthy controls. hsa_circRNA_404013 was significantly positively correlated with nasal discharge, nasal itching, the total nasal symptoms of AR, and brain-derived neurotrophic factor (BDNF) expression level in peripheral blood. Receiver operating characteristic curve analysis results showed that hsa_circRNA_404013 may be used as peripheral blood circulating marker for the diagnosis of AR with the area under curve of 0.8499 (95% CI: 0.783–0.916). In further bioinformatics analysis, hsa_circRNA_404013 may regulate the expression of BDNF through hsa-mir-182-5p contributing to the pathogenesis of AR. Conclusion: The expression profile of circRNAs from the peripheral blood sample of AR patients was obtained. The expression of hsa_circRNA_404013 was significantly up-regulated in the peripheral blood of AR patients, which may be used as a circulating marker for AR patients. Furthermore, hsa_circRNA_404013 may regulate the expression level of BDNF through hsa-mir-182-5p in AR pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peripheral Circular RNA Profiling from Patients with Allergic Rhinitis Identified hsa_circRNA_404013 as a Potential Diagnostic Biomarker

Peng

Sun

Zhang

et al. 2022

Int Arch Allergy Immunol

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“…circRNA, an emerging endogenous ncRNA, also plays a critical role in the immune and inflammatory responses [ 150 , 151 ]. Chen et al [ 152 ] identified circRNA expression profiles in the nasal mucosa of AR mice using RNA sequencing and found 51 circRNAs upregulated and 35 circRNAs downregulated, with some circRNAs involved in activating T and B cells. In another study, investigators analyzed circRNAs in the nasal mucosa of AR patients and controls using high-throughput sequencing.…”

Section: Ncrna and Armentioning

confidence: 99%

The Role of Noncoding RNA in Airway Allergic Diseases through Regulation of T Cell Subsets

Cheng

Tang

Xie

et al. 2022

Mediators of Inflammation

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Allergic rhinitis and asthma are common airway allergic diseases, the incidence of which has increased annually in recent years. The human body is frequently exposed to allergens and environmental irritants that trigger immune and inflammatory responses, resulting in altered gene expression. Mounting evidence suggested that epigenetic alterations were strongly associated with the progression and severity of allergic diseases. Noncoding RNAs (ncRNAs) are a class of transcribed RNA molecules that cannot be translated into polypeptides and consist of three major categories, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). Previous studies showed that ncRNAs were involved in the physiopathological mechanisms of airway allergic diseases and contributed to their occurrence and development. This article reviews the current state of understanding of the role of noncoding RNAs in airway allergic diseases, highlights the limitations of recent studies, and outlines the prospects for further research to facilitate the clinical translation of noncoding RNAs as therapeutic targets and biomarkers.

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“…Then cytokines expression was checked with a BeyoFast™ SYBR Green One-Step qRT-PCR kit (D7268M; Beyotime, China) by following the kit's protocol. 20 β-actin serves as an internal control to determine the relative expression of specific genes, and the primers are provided in Table 1.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qrt-pcr)mentioning

confidence: 99%

Orientin inhibits the progression of fibroblast-like synovial cells in rheumatoid arthritis by regulating MAPK-signaling pathway

Wang²

2022

Allergol Immunopathol

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Background: Natural compounds are found to play an essential role in diverse inflammatory diseases, including rheumatoid arthritis (RA). Orientin, a flavonoid compound, is closely related to diverse pathological processes. Nevertheless, the role of orientin in RA is still unknown. Methods: The cell viability was tested through cell counting kit 8 (CCK-8) assay, and the number of cell colonies was calculated via colony formation assay. In addition, flow cytom-etry assay was employed to detect apoptosis rate in human RA fibroblast-like synoviocytes (RA-FLS). Besides, Transwell assay was introduced to determine cell migratory and invasive abilities. Moreover, the level of cytokines (IL-8, IL-1β, and IL-6) was estimated with quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent serologic assay. Furthermore, western blotting analysis was used to test the protein levels of cleavedcaspase-3, Bax, BCL-2, matrix metalloproteinase (MMP)-2, MMP-9, phosphorylated c-Jun N-terminal kinase, p-P38, and phospho-extracellular signal-related kinase. Results: Orientin inhibited cell viability, migration as well as invasion in a concentration-dependent manner in human RA-FLS. Additionally, treatment of orientin facilitated apoptosis and decreased the secretion of cytokines induced by tumor necrosis factor alpha (TNF-α) in human RA-FLS. Moreover, orientin inactivated mitogen-activated protein kinase (MAPK)-related signaling pathway, notably in human RA-FLS. Conclusion: These findings confirmed that orientin inhibited human RA-FLS development and decreased TNF-α-induced inflammatory factors, at least partly, by modulating MAPK-signaling pathway, which implied that orientin might be an effective agent for treating RA.

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Altered circular RNA expression profiles in an ovalbumin-induced murine model of allergic rhinitis

Cited by 4 publications

References 29 publications

Peripheral Circular RNA Profiling from Patients with Allergic Rhinitis Identified hsa_circRNA_404013 as a Potential Diagnostic Biomarker

Peripheral Circular RNA Profiling from Patients with Allergic Rhinitis Identified hsa_circRNA_404013 as a Potential Diagnostic Biomarker

The Role of Noncoding RNA in Airway Allergic Diseases through Regulation of T Cell Subsets

Orientin inhibits the progression of fibroblast-like synovial cells in rheumatoid arthritis by regulating MAPK-signaling pathway

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