Altered chemokine receptor expression in the peripheral blood lymphocytes in polymyositis and dermatomyositis

Zhu, Ziwei; Yang, Chunshu; Wang, Jianbo; Feng, Qianhui; Chen, Qi; Yang, Pingting

doi:10.1016/j.cyto.2017.08.018

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…For instance, the aberrant neutrophil extracellular trap (NET) formation may be involved in the pathogenesis of ILD in DM patients ( Peng et al, 2018 ), and proteinase 3, one type of neutrophil serine proteinases, is lower in patients of DM without ILD ( Gao et al, 2018 ). We also confirmed previous observations related to the pathogenesis of DM and DM-ILD, including the presence of the cytokine–cytokine receptor interaction ( Zhu et al, 2017 ; Chen et al, 2018 ), IL−17 signaling pathway ( Nasr et al, 2012 ). Additionally, we observed that complement and coagulation cascade pathway may play a critical role in DM-ILD.…”

Section: Discussionsupporting

confidence: 91%

Transcriptome Sequencing Identifies PLAUR as an Important Player in Patients With Dermatomyositis-Associated Interstitial Lung Disease

et al. 2021

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Dermatomyositis (DM), an inflammatory disorder, is often associated with interstitial lung disease (ILD). However, the underlying mechanism remains unclear. Our study performed RNA sequencing (RNA-seq) and integrative bioinformatics analysis of differentially expressed genes (DEGs) in patients with dermatomyositis-associated interstitial lung disease (DM-ILD) and healthy controls. A total of 2,018 DEGs were identified between DM-ILD and healthy blood samples. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that DEGs were mainly involved in immune- and inflammatory-related biological processes and pathways. Disease ontology (DO) enrichment analysis identified 35 candidate key genes involved in both skin and lung diseases. Meanwhile, a total of 886 differentially expressed alternative splicing (AS) events were found between DM-ILD and healthy blood samples. After overlapping DEGs with differential AS genes, the plasminogen activator and urokinase receptor (PLAUR) involved in immune-related biological processes and complement and coagulation cascades was screened and identified as the most important gene associated with DM-ILD. The protein–protein interaction (PPI) network revealed that PLAUR had interactions with multiple candidate key genes. Moreover, we observed that there were significantly more neutrophils and less naive B cells in DM-ILD samples than in healthy samples. And the expression of PLAUR was significantly positively correlated with the abundance of neutrophils. Significant higher abundance of PLAUR in DM-ILD patients than healthy controls was validated by RT-qPCR. In conclusion, we identified PLAUR as an important player in regulating DM-ILD by neutrophil-associated immune response. These findings enrich our understanding, which may benefit DM-ILD patients.

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Section: Discussionsupporting

confidence: 91%

Transcriptome Sequencing Identifies PLAUR as an Important Player in Patients With Dermatomyositis-Associated Interstitial Lung Disease

et al. 2021

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“…Western blot analysis. The cell lines were lysed using a whole protein extraction kit (Nanjing KeyGen Biotech, Co., Ltd., Nanjing, China (19). FlowJo software version 10 (TreeStar, Inc., Ashland, OR, USA) was used for flow cytometry analysis.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

Novel tumor‑suppressor FOXN3 is downregulated in adult acute myeloid leukemia

Zhang

et al. 2019

Oncol Lett

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Forkhead box protein N3 (FOXN3) is a transcriptional repressor involved in cell cycle regulation and tumorigenesis. Abnormalities in gene structure and epigenetics of FOXN3 are closely associated with the occurrence of hematological malignancies; however, its involvement in the pathogenesis of acute myeloid leukemia (AML) remains unknown. The present study aimed to examine the potential significance of FOXN3 in AML. FOXN3 expression levels were examined in patients with AML and AML cell lines, and its clinical significance in AML was evaluated. FOXN3-overexpressing AML cell lines were established, and the biological function of FOXN3 was detected by flow cytometry and a Cell Counting Kit-8 assay. A significant decrease in FOXN3 expression levels was observed in patients with AML and in the AML cell lines in vitro. FOXN3 expression levels were associated with the number of leukocytes in patients. FOXN3 overexpression may inhibit cell proliferation in AML cell lines, induce cell cycle S-phase arrest and promote apoptosis in OCI-AML3 and THP-AML cells. The present study provided insight into how FOXN3 may serve as a novel tumor suppressor in AML.

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“…1 In PM, the perivascular infiltration in muscle biopsies is composed predominantly of CD8 + T cells and macrophages, while inflammatory infiltration in DM mainly consists of CD4 + T cells, B cells, and macrophages. 2 Interleukin-17 (IL-17), a cytokine produced by T-helper cells, is involved in PM/DM pathogenesis via IL-17/intercellular adhesion molecular 1 pathway, and can culminate in PM/DM deterioration. Regenerating islet-derived protein 3-alpha (REG3A) is an antimicrobial protein which controls bacteria proliferation.…”

Section: Introductionmentioning

confidence: 99%

Downregulated MiR-206 expression promotes the proliferation and migration of macrophages by regulating IL-17A/REG3A pathway

Huang

Gong

et al. 2020

Eur J Inflamm

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This study sought to investigate the role of miR-206 in polymyositis/dermatomyositis (PM/DM) development. Transwell and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay were performed to investigate cell migration and proliferation. Real-time polymerase chain reaction (PCR) analysis was used to determine the expression of miR-206, interleukin-17A (IL-17A), IL-17 receptor A (IL-17RA), and regenerating islet-derived protein 3-alpha (REG3A). Significantly, miR-206 mimics decreased macrophage migration and proliferation, while miR-206 inhibitors exhibited the opposite effects. Indeed, elevating IL-17RA levels resulted in increased REG3A expression, which was inhibited by IL-17RA siRNA. Besides, miR-206 mimics decreased IL-17A and REG3A expressions, but miR-206 inhibitors showed opposite effects. Moreover, miR-206 expression in PM/DM patients was significantly reduced compared with the healthy controls, while IL-17A and REG3A expressions substantially increased among PM/DM patients. These findings suggested that downregulation of miR-206 increased the migration and proliferation of macrophages via IL-17A/REG3A signaling pathway, which could promote the inflammatory infiltration in PM/DM.

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Altered chemokine receptor expression in the peripheral blood lymphocytes in polymyositis and dermatomyositis

Cited by 6 publications

References 29 publications

Transcriptome Sequencing Identifies PLAUR as an Important Player in Patients With Dermatomyositis-Associated Interstitial Lung Disease

Transcriptome Sequencing Identifies PLAUR as an Important Player in Patients With Dermatomyositis-Associated Interstitial Lung Disease

Novel tumor‑suppressor FOXN3 is downregulated in adult acute myeloid leukemia

Downregulated MiR-206 expression promotes the proliferation and migration of macrophages by regulating IL-17A/REG3A pathway

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