Altered CD8+ T cell frequency and function in tuberculous lymphadenitis

Kumar, Nathella Pavan; Ramaswamy, Sridhar; Hanna, Luke Elizabeth; Banurekha, Vaithilingam V.; Jawahar, M S; Nutman, Thomas B.; Babu, Subash

doi:10.1016/j.tube.2014.06.007

Cited by 9 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, very little is known about the association of these cytokines in the immune responses engendered in TBL. Previous studies have shown that TBL is characterized by an expansion of mono- and multi-functional Th1 and Th17 cells in response to TB antigens and that CD8 + T cells expressing Type 1 and Type 17 cytokines are also increased in comparison to pulmonary TB [9, 10]. Similarly, enhanced concentrations of circulating IL-10, IL-8 and TNFβ have been described as potential blood biomarkers in TBL [11], while level of IFNγ, TNFα, GM-CSF, IL-1β, IL-2, IL-4 and IL-6 have been shown to be elevated in adult TBL compared to children with TBL [12].…”

Section: Discussionmentioning

confidence: 99%

Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1β and IL-18 in tuberculous lymphadenitis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Background Type 1, Type 17 and other pro-inflammatory cytokines are known to play an important role in resistance to pulmonary tuberculosis. The role of these cytokines in tuberculous lymphadenitis (TBL) is not well characterized. Methods To estimate the systemic and mycobacterial antigen – stimulated cytokine concentrations of Type 1, Type 17, other pro-inflammatory and regulatory cytokines in TBL, we examined both the systemic and the antigen–specific concentrations of these cytokines in TBL (n = 31) before and after chemotherapy and compared them with those with latent tuberculosis infection (LTB, n = 31). Results We observed significantly reduced systemic concentrations of the pro-inflammatory cytokines – IL-1β and IL-18 but not other Type 1 or Type 17 cytokines in TBL compared to LTB. Following standard anti-tuberculosis (TB) treatment, we observed a significant increase in the concentrations of both IL-1β and IL-18. In addition, we also observed significantly reduced baseline or mycobacterial – antigen or mitogen stimulated concentrations of IL-1β and IL-18 in TBL individuals. Similar to systemic cytokine concentrations, anti-TB treatment resulted in significantly increased concentrations of these cytokines following antigen stimulation. Conclusions TBL is therefore, characterized by reduced systemic and antigen – specific concentrations of IL-1β and IL-18, which are reversible following anti-TB treatment, indicating that these cytokines are potential correlates of protective immunity in TBL.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1β and IL-18 in tuberculous lymphadenitis

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, regional (i.e., Asian vs. Western countries) and/or racial differences in the roles of various Th cell subsets in the local inflammatory reaction in NP tissues have been reported 7 . Similar to the subsets of CD4 + T cells, the subsets of CD8 + T cells, including Tc1, Tc2, and Tc17 cells, and their interrelated cytokines have been shown to play an important role in the adaptive immune response to pathogens in various infectious and inflammatory diseases, including HIV infection 8 , rheumatic diseases 9 and tuberculosis 10 . In airway diseases, CD8 + T cells were found to suppress allergen-induced late airway responses, inhibit airway eosinophilia through secretion of IFN-γ and mediate pulmonary alveolitis and inflammation 11 12 .…”

mentioning

confidence: 99%

Phenotypic and functional characteristics of IL-21-expressing CD8+ T cells in human nasal polyps

Xiao

Jia

Bai

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Although CD4+ T cells are recognized to play an important role in the inflammatory response of nasal polyps (NPs), the biological functions of CD8+ T cells in polypogenesis remain unclear. In this study, we analyzed cell markers, cytokine expression and transcription factors in IL-21-expressing CD8+ T cells in polyp tissues of NP patients. The results showed that the majority of IL-21-producing CD8+ T cells were effector memory cells and they co-expressed IFN-γ. IL-21-expressing CD8+ T cells in polyp tissues expressed higher CXCR5, PD-1, and ICOS levels than cells in control tissues and showed significantly higher T-bet and Bcl-6 expression levels compared with IL-21−CD8+ T cells. Purified polyp CD8+ T cells promoted IgG production from isolated polyp B cells in vitro, and recombinant IL-12 modulated the expression of IL-21, IFN-γ and CD40L in purified polyp CD8+ T cells. Moreover, the percentage of IL-21+CD8+ T cells in polyp tissues was positively correlated with endoscopic and CT scan scores in NP patients. These findings indicated that polyp CD8+ T cells, by co-expressing IL-21 and IFN-γ and other markers, display a Tfh cell functionality, which is associated with the clinical severity of NP patients.

show abstract

“…Phorbol 12-myristate 13-acetate (PMA)-ionomycin (PMA-Iono; Calbiochem) was used as a positive-control stimulus at final concentrations of 12.5 ng/ml and 125 ng/ml. Dose-response studies have been performed previously, and the optimal concentrations were derived from the previous studies (4,5).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that TBL is characterized by a mycobacterial antigen-specific expansion of mono-and multifunctional Th1 and Th17 cells, as well as increased frequencies of CD8 ϩ T cells expressing type 1 and type 17 cytokines in comparison to those in individuals with PTB (4,5). In contrast, TBL is associated with reduced systemic and antigen-stimulated production of certain proinflammatory cytokines, including interleukin-1␤ (IL-1␤) and IL-18 (6), and elevated systemic levels of others, including IL-8 and tumor necrosis factor beta (TNF-␤) (7).…”

mentioning

confidence: 99%

Tuberculous Lymphadenitis Is Associated with Enhanced Baseline and Antigen-Specific Induction of Type 1 and Type 17 Cytokines and Reduced Interleukin-1β (IL-1β) and IL-18 at the Site of Infection

Kathamuthu¹,

Moideen²,

Baskaran

et al. 2017

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Tuberculous lymphadenitis (TBL) is characterized by an expansion of Th1 and Th17 cells with altered serum levels of proinflammatory cytokines. However, the cytokine profile at the site of infection, i.e., the affected lymph nodes, has not been examined in detail. To estimate the baseline and mycobacterial antigenstimulated concentrations of type 1, type 17, and other proinflammatory cytokines in patients with TBL (n ϭ 14), we examined both the baseline and the antigen-specific concentrations of these cytokines before and after chemotherapy and compared them with those in individuals with pulmonary tuberculosis (PTB) (n ϭ 14). In addition, we also compared the cytokine responses in whole blood and those in the lymph nodes of TBL individuals. We observed significantly enhanced baseline and antigen-specific levels of type 1 cytokines (gamma interferon [IFN-␥] and tumor necrosis factor alpha [TNF-␣]) and a type 17 cytokine ) and significantly diminished baseline and antigen-specific levels of proinflammatory cytokines (IL-1␤ and IL-18) in the whole blood of TBL individuals compared to those in the whole blood of PTB individuals. Moreover, we also observed a pattern of baseline and antigen-specific cytokine production at the site of infection (lymph node) similar to that in the whole blood of TBL individuals. Following standard antituberculosis (anti-TB) treatment, we observed alterations in the baseline and/or antigen-specific levels of IFN-␥, TNF-␣, IL-1␤, and IL-18. TBL is therefore characterized by enhanced baseline and antigen-specific production of type 1 and type 17 cytokines and reduced baseline and antigen-specific production of IL-1␤ and IL-18 at the site of infection.KEYWORDS tuberculosis, lymphadenitis, cytokines I mmune responses in tuberculous lymphadenitis (TBL) are poorly understood, although TBL is the most common form of extrapulmonary TB, accounting for 30 to 40% of cases (1). TBL commonly affects the superficial lymph nodes (LN) of the body, with the cervical lymph nodes being the most commonly affected, followed by the inguinal, axillary, mesenteric, mediastinal, and intramammary lymph nodes (2). The pathogenesis of TBL and/or the pathway of dissemination from the lungs is still unclear,

show abstract

Altered CD8+ T cell frequency and function in tuberculous lymphadenitis

Cited by 9 publications

References 35 publications

Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1β and IL-18 in tuberculous lymphadenitis

Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1β and IL-18 in tuberculous lymphadenitis

Phenotypic and functional characteristics of IL-21-expressing CD8+ T cells in human nasal polyps

Tuberculous Lymphadenitis Is Associated with Enhanced Baseline and Antigen-Specific Induction of Type 1 and Type 17 Cytokines and Reduced Interleukin-1β (IL-1β) and IL-18 at the Site of Infection

Contact Info

Product

Resources

About