Altered carbohydrate composition in colorectal adenomas and carcinomas: Histochemical characterization of N-acetylgalactosamine,l-fucose, and o-acetylated sialic acid

Abstract

Changes of surface sugar residues in the large intestinal mucosa may be associated with malignant transformation and may be of importance in differentiating borderline lesions. To compare these changes in normal mucosa, adenomas, and carcinomas of the large intestine, we investigated modifications of carbohydrate composition, such as those of N-acetyl-galactosamine (GalNac), L-fucose, and o-acetylated sialic acid, by histochemical staining with Dolichos biflorus agglutinin (DBA) and Ulex europaeus agglutinin-1 (UEA-1) lectins, and with Culling's periodic acid-thionin Schiff/potassium hydroxide/periodic acid-Schiff (PAT/KOH/PAS), respectively. For stable staining, the sections stained with DBA and UEA-1 were pretreated with potassium hydroxide and neuraminidase. We conclude that the pattern of the two lectin stainings in carcinomas is quite different from that in normal mucosa and adenomas, and that it shows the carcinomatous features in some cases of adenoma with severe atypia (borderline lesions). In contrast, PAT/KOH/PAS staining demonstrates differences between normal mucosa and adenomas rather than differences between adenomas and carcinomas.

Application of near-infrared spectroscopy for the diagnosis of colorectal cancer in resected human tissue specimens

“…As the colorectal cancerous tissues mainly differ from those of normal ones in fatty acids, carbohydrates, glycosaminoglycans, glycoproteins and glycolipids composition [29][30][31][32][33][34][35][36][37][38][39], the CH vibrational regions in particular are representing these classes in a prominent way [6,15,26,43,44]. The spectral intervals of the Fig.…”

“…Altered compositions in fatty acids [29][30][31], carbohydrates [32], glycosaminoglycans [33,34], glycoproteins [35][36][37] and glycolipids [38,39] were found in adenoma and carcinoma of colorectal tissues. These different tissue compositions will contribute to the spectral variance between cancer and normal tissue samples.…”

The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling

“…For neuraminidase treatment as previously described [6], the deparaffinized sections were washed with 0.1 M sodium acetate buffer, pH 5.5, and incubated at 37C for 5 h with neuraminidase at a concentration of 1 U/ml in 0.1 M sodium acetate buffer, pH 5.5, and 0.04 M calcium chloride. Sections were then washed with 0.01 M PBS and lectin staining was carried out as described above.…”

Mannan-Binding Protein, a C-Type Serum Lectin, Recognizes Primary Colorectal Carcinomas through Tumor-Associated Lewis Glycans

“…In other words, MBP ligands are expressed on cancer tissues of human primary colorectal carcinoma mucosae. ) glycans expressed on a broad spectrum of foreign pathogens, only the fucose expression was upregulated in most studies on colorectal carcinomas (19)(20)(21). To determine whether a fucose moiety mediates the interaction between MBP and cancer tissues, we performed MBP histochemistry in the presence of a plant lectin: Con A or AAL.…”