Altered B lymphocyte homeostasis and functions in systemic sclerosis

Forestier, Alexandra; Guerrier, Thomas; Jouvray, Mathieu; Giovannelli, Jonathan; Lefèvre, Guillaume; Sobanski, Vincent; Hauspie, C.; Hachulla, É.; Hatron, Pierre‐Yves; Zephir, Hélène; Vermersch, Patrick; Labalette, Myriam; Launay, David; Dubucquoi, Sylvain

doi:10.1016/j.autrev.2017.10.015

Cited by 64 publications

(64 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although randomized double-blind clinical trials examining the utility of B cell depletion therapy in SSc have not been performed, some studies have reported clinical improvement with rituximab, especially in the context of early diffuse SSc (22,23). Additionally, activated B cells have been observed in the circulation of SSc patients (24,36). Although there may be a contribution of infiltrating B cells to pathology in SSc, we focused on the T cells in the infiltrate across this group of patients given their relative preponderance in the tissue.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis

Mitsui

Kaneko

Perugino

et al. 2020

Journal of Clinical Investigation

133

114

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis

Mitsui

Kaneko

Perugino

et al. 2020

Journal of Clinical Investigation

133

114

View full text Add to dashboard Cite

“…It is possible that these B cells are linked with the production of higher affinity antibodies relevant in inflammation. Indeed, subsets of memory B cell shave been reported to dysregulated in other autoimmune diseases such as systemic lupus erythematosus and multiple sclerosis[18]. Based on the reported pathogenic role of B cell in other autoimmune diseases[11], we anticipated that also plays an important role in etiology of IgAV.…”

Section: Discussionmentioning

confidence: 98%

Altered B cells homeostasis in child-onset immunoglobulin A vasculitis

Liu

Jiang

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

46Background: Immunoglobulin A vasculitis (IgAV), also called Henoch-Schönlein purpura, is 47 a systemic small vessels vasculitis with immunoglobulin A1-dominant immune deposits. B-48 cells are a heterogeneous population with unique subsets distinguished by their phenotypes and 49 cytokine production. Here, we explored the status of B cell subsets in patients with IgAV. 50 Methods: Thirty IgAV patients and fifteen age-and sex-matched healthy individuals were 51 enrolled in this study. Fresh blood samples were collected from both healthy and IgAV patients. 52 Upon the distinct expressions of CD3, CD19, CD20, CD38, CD27 and IgD, peripheral blood 53 mononuclear cells (PBMCs) were initially categorized into plasmablasts and memory B cells. 54 Subsequently, using surface markers including CD138 and IgM, and intracellular markers 55 containing IgM and IgG, plasmablasts and memory B cells were further divided into distinct 56 subgroups. A total of eleven populations were detected using multiple flow cytometry. 59 HCs. Only CD3 -CD19 + IgD -CD27 + IgM + B cell counts were reduced in IgAV. The elevated B 60 cell numbers returned to normal after treatment. Plasma and plasmablast B cell numbers 61 correlated with plasma IgA levels. On the contrary, CD3 -CD19 + IgD -CD27 + IgM + B cell 62 numbers were negatively proportional to the plasma IgA levels while naïve B cell numbers 63 correlated with plasma and plasmablast B cell counts.64 Conclusions: We hypothesized that immunoglobulin production was abnormally elevated in 65 IgAV and could be explained by altered B-cell subset homeostasis.66

show abstract

“…Both subsets expressed a high level expression of ectopic membrane hMSH2 proteins. CD25, the interleukin‐2 receptor alpha chain, is one of the activation biomarkers for human B‐cells . It has been reported serving as a negative growth regulator, a novel marker and potential drug target in chronic myeloid leukaemia .…”

Section: Discussionmentioning

confidence: 99%

EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances γδT‐cell‐mediated cytotoxicity via TCR and NKG2D

Dai

Liu

et al. 2018

Immunology

View full text Add to dashboard Cite

The engagement of Epstein-Barr virus (EBV)-induced protein ligands in γδ T-cell-mediated anti-EBV immunity, especially in EBV-associated B-cell malignancies, has not been fully elucidated. Previously we reported the overexpression of human MutS homologue 2 (hMSH2), a stress-inducible protein ligand for human γδ T-cells, on EBV-transformed B lymphoblastic cell lines (B-LCLs). In this study, we first generated EBV-transformed B-LCLs from peripheral blood mononuclear cells of healthy volunteers with B95-8 cellular supernatant and cyclosporine A. Secondly, we demonstrated the significantly elevated cell surface protein expression and mRNA transcription of hMSH2 in EBV-transformed B-LCLs, 3D5 and EBV-positive B lymphoma cell line Daudi and Raji. Thirdly, hMSH2-mediated recognition of EBV-transformed B malignant cells by human γδ T-cells was confirmed by specific antibody blocking and siRNA interference. Both TCRγδ and NKG2D participated in hMSH2-mediated recognition of EBV-transformed B malignant cells. Furthermore, hMSH3 and hMSH6, the companion proteins of hMSH2, along with CD98, were found overexpressed on the surface of EBV-transformed malignant B-cells. We concluded that the induced overexpression of hMSH proteins might serve as early alerting biomarkers emerged in EBV-related B-cell malignances or as potential targets for establishing γδ T-cell-based therapeutic immunotherapies towards EBV infection.

show abstract

Altered B lymphocyte homeostasis and functions in systemic sclerosis

Cited by 64 publications

References 67 publications

Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis

Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis

Altered B cells homeostasis in child-onset immunoglobulin A vasculitis

EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances γδT‐cell‐mediated cytotoxicity via TCR and NKG2D

Contact Info

Product

Resources

About