Altered antigenicity is seen in the lipopolysaccharide profile of non-serotypeable Actinobacillus actinomycetemcomitans strains

Paju, Susanna

doi:10.1016/s0928-8244(99)00188-1

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…Paju et al (14) reported that both serotypeable and nonserotypeable A. actinomycetemcomitans strains belong to identical AP-PCR genotypes. It is possible that some of these nonserotypeable strains are derived from serotypeable strains (14). Our multiplex PCR test may be useful for identifying the origin of nonserotypeable A. actinomycetemcomitans strains.…”

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confidence: 99%

Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCR

et al. 2001

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confidence: 99%

Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCR

et al. 2001

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“…The strains were serotypeable or non-serotypeable to lack of serotype Antigen in A. actinomycetemcomitans rEsEArch rEPOrts clinical J Dent Res 89 (3) 2010 Lack of Serotype Antigen Expression 293 serotypes a-e by immunodiffusion assay and were selected from our strain collection to represent various genotypes as determined by arbitrarily primed PCR Dogan et al, 1999;Paju et al, 2000b) (Appendix Table 1). When available, several non-serotypeable strains per individual were included.…”

Section: Materials and Methods Bacterial Strainsmentioning

confidence: 99%

Lack of Serotype Antigen in A. actinomycetemcomitans

et al. 2010

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Aggregatibacter actinomycetemcomitans is divided into 6 serotypes. Occurrence of non-serotypeable strains is known, but background reasons are unclear. We hypothesized that non-serotypeable strains represent new serotypes or have altered expression of serotype-specific polysaccharide antigen (S-PA). We first characterized 311 strains from 189 individuals using both immunoassay- and PCR-based serotyping. Next, using natural human infection and rabbit immunization approaches, we clarified whether the phenotypically non-serotypeable strains expressed S-PA. Immunoassay identified serotypes a-f among 216 strains from 159 individuals. The remaining 95 strains from 30 individuals were phenotypically non-serotypeable. Yet, all these strains were identified by PCR-typing as serotype a-, b-, c-, or f. Non-serotypeability was confirmed by Western immunoblot with respective rabbit antisera. Patient sera remained non-reactive with autologous non-serotypeable strains at the serotype-specific region. Rabbit immunization with a phenotypically non-serotypeable strain induced no antibody production against S-PA. Thus, phenotypically non-serotypeable strains did not include novel serotypes, but lacked S-PA expression.

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“…LPSs of the A. actinomycetemcomitans strains were isolated as previously described (21). Briefly, the strains were grown on Trypticase soy agar (Difco Laboratories, Detroit, Mich.) plates in 5% CO 2 in air at 37°C for 2 to 3 days.…”

Section: Methodsmentioning

confidence: 99%

Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide

Lakio

Paju

Alfthan³

et al. 2003

Infect Immun

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Actinobacillus actinomycetemcomitans is a gram-negative, facultatively anaerobic bacterium which is associated especially with aggressive forms of periodontitis. Contradictory results on the localization of the A. actinomycetemcomitans serotype-specific antigen have been reported. The aim of the present study was to characterize the A. actinomycetemcomitans serotype d-specific antigen. The antigen was isolated by affinity chromatography. The affinity column was prepared from immunoglobulin G isolated from rabbit antiserum raised against A. actinomycetemcomitans serotype d. The isolated antigen was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and silver staining, all of which revealed a ladder-like structure typical for the O antigen of lipopolysaccharide (LPS). In a displacement enzyme-linked immunosorbent assay (ELISA), the isolated antigen displaced in a concentration-dependent manner the binding of the polyclonal rabbit antiserum raised against A. actinomycetemcomitans serotype d to the competing whole-cell serotype d antigen. The isolated antigen contained LPS, and an equal concentration of LPS isolated from A. actinomycetemcomitans serotype d gave a similar displacement curve in the ELISA. In order to test the immunogenic properties of the isolated antigen, it was used to immunize a rabbit. The antiserum raised against the isolated antigen displayed specificity in Western blotting and ELISA similar to that of antibody raised against LPS isolated from A. actinomycetemcomitans serotype d. In conclusion, our results show that the A. actinomycetemcomitans serotype d-specific antigen contains the O-antigenic structure of LPS.

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Altered antigenicity is seen in the lipopolysaccharide profile of non-serotypeable Actinobacillus actinomycetemcomitans strains

Cited by 5 publications

References 23 publications

Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCR

Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCR

Lack of Serotype Antigen in A. actinomycetemcomitans

Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide

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