Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio

Heredia-García, Gerardo; Gómez-Oliván, Leobardo Manuel; Orozco-Hernández, José Manuel; Luja-Mondragón, Marlenee; Islas-Flores, Hariz; SanJuan-Reyes, Nely; Galár-Martínez, Marcela; García-Medina, Sandra; Dublán‐García, Octavio

doi:10.1016/j.scitotenv.2019.07.165

Cited by 18 publications

(7 citation statements)

References 69 publications

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“…Similar data in direct connection with OS were also observed in the case of other sweeteners such as saccharin, sucralose, and cyclamate, with a similar effect on the level of peroxidized lipids, GSH, but also on 8-hydroxy-20-deoxyguanosine (8-OHdG), without describing a clear mechanism of ROS generation [ 152 , 153 , 154 , 155 ].…”

Section: From Pure Caffeine To Coffee Productssupporting

confidence: 59%

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Ősz

Jîtcă²,

Ștefănescu³

et al. 2022

IJMS

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Caffeine is the most frequently used substance with a central nervous system stimulant effect, but its consumption is most often due to the intake of foods and drinks that contain it (coffee, tea, chocolate, food supplements with plant extracts of Guarana, Mate herba, Cola nuts). Due to its innocuity, caffeine is a safe xanthine alkaloid for human consumption in a wide range of doses, being used for its central nervous stimulating effect, lipolytic and diuresis-enhancing properties, but also as a permitted ergogenic compound in athletes. In addition to the mechanisms that explain the effects of caffeine on the targeted organ, there are many proposed mechanisms by which this substance would have antioxidant effects. As such, its consumption prevents the occurrence/progression of certain neurodegenerative diseases as well as other medical conditions associated with increased levels of reactive oxygen or nitrogen species. However, most studies that have assessed the beneficial effects of caffeine have used pure caffeine. The question, therefore, arises whether the daily intake of caffeine from food or drink has similar benefits, considering that in foods or drinks with a high caffeine content, there are other substances that could interfere with this action, either by potentiating or decreasing its antioxidant capacity. Natural sources of caffeine often combine plant polyphenols (phenol-carboxylic acids, catechins) with known antioxidant effects; however, stimulant drinks and dietary supplements often contain sugars or artificial sweeteners that can significantly reduce the effects of caffeine on oxidative stress. The objective of this review is to clarify the effects of caffeine in modulating oxidative stress and assess these benefits, considering the source and the dose administered.

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Section: From Pure Caffeine To Coffee Productssupporting

confidence: 59%

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Ősz

Jîtcă²,

Ștefănescu³

et al. 2022

IJMS

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“…Heart rate is controlled by signals from the brain, therefore, damage in the brain may cause alterations in heart rate regulation [ 81 , 82 , 83 ]. A previous study in common carp also suggested lower antioxidant capacity after sucralose exposure [ 84 ]. A decrease in antioxidant and an increase in catalase is a marker for ROS levels, which can cause tissue damage, especially in sensitive organs such as the heart.…”

Section: Discussionmentioning

confidence: 87%

Acute and Sub-Chronic Exposure to Artificial Sweeteners at the Highest Environmentally Relevant Concentration Induce Less Cardiovascular Physiology Alterations in Zebrafish Larvae

Saputra

Lai

Fernández

et al. 2021

Biology

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Artificial sweeteners are widely used food ingredients in beverages and drinks to lower calorie intake which in turn helps prevent lifestyle diseases such as obesity. However, as their popularity has increased, the release of artificial sweetener to the aquatic environment has also increased at a tremendous rate. Thus, our study aims to systematically explore the potential cardiovascular physiology alterations caused by eight commercial artificial sweeteners, including acesulfame-K, alitame, aspartame, sodium cyclamate, dulcin, neotame, saccharine and sucralose, at the highest environmentally relevant concentration on cardiovascular performance using zebrafish (Danio rerio) as a model system. Embryonic zebrafish were exposed to the eight artificial sweeteners at 100 ppb and their cardiovascular performance (heart rate, ejection fraction, fractional shortening, stroke volume, cardiac output, heartbeat variability, and blood flow velocity) was measured and compared. Overall, our finding supports the safety of artificial sweetener exposure. However, several finding like a significant increase in the heart rate and heart rate variability after incubation in several artificial sweeteners are noteworthy. Biomarker testing also revealed that saccharine significantly increase the dopamine level in zebrafish larvae, which is might be the reason for the cardiac physiology changes observed after saccharine exposure.

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“…Although studies in human cells are scarce, studies in the common carp Cyprinus carpio also point to cytotoxicity in concentrations of 0.05–155 µg/L 8 . Concerning cell viability, we do not expect adverse effects, since other studies use sucralose to improve the proliferation of specific cell lines, such as HFF‐1 9…”

Section: Resultsmentioning

confidence: 95%

“…Again, human cell studies are unusual. In common carp ( Cyprinus carpio ), sucralose showed an increase in DNA damage of up to 35% in food concentrations 8 . In in vivo studies, sucralose can lead to inflammatory conditions in the liver, generating excess free radicals and subsequent inflammatory process with DNA damage 12 …”

Section: Resultsmentioning

confidence: 99%

Sucralose causes non‐selective CD4 and CD8 lymphotoxicity via probable regulation of the MAPK8/APTX/EID1 genes: An in vitro/in silico study

Pasqualli

Chaves

Pereira

et al. 2020

Clin Exp Pharma Physio

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One of the most widely used sweeteners in the world is sucralose. With sweetening power 600 times greater than sucrose, its use grows among those who seek to cut calories. Research shows that when heated, sucralose generates toxic products that attack the organism and interact with DNA. Our objective was to test this sweetener under unheated conditions and at average concentrations of consumption, evaluating parameters of cytotoxicity, genotoxicity, and immunotoxicity. For this purpose, we made use of lymphocyte cultures and the analysis of their CD3+, CD4+, and CD8+ subpopulations. In a complementary way, the mechanism of action is proposed here by computational methods. Our results showed that sucralose reduces non‐selectively the total lymphocytes due to falls in the levels of the CD4+, CD8+, and CD4+CD8+ subpopulations. We observed an increase in the level of DNA damage and a gradual incidence of structural changes in the lymphocyte chromosomal sets. It was possible to propose that sucralose modulates the gene expression, interfering especially with the MAPK8, APTX, and EID1 genes. This article presents the results of an evidence‐based approach to the safety of human health in the use of sucralose. Finally, this study points out that sucralose has cytotoxic, genotoxic, and mutagenic effects in the concentrations and conditions tested in human lymphocyte cell culture.

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Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio

Cited by 18 publications

References 69 publications

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Acute and Sub-Chronic Exposure to Artificial Sweeteners at the Highest Environmentally Relevant Concentration Induce Less Cardiovascular Physiology Alterations in Zebrafish Larvae

Sucralose causes non‐selective CD4 and CD8 lymphotoxicity via probable regulation of the MAPK8/APTX/EID1 genes: An in vitro/in silico study

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