Alterations of the human gut Methanobrevibacter smithii as a biomarker for inflammatory bowel diseases

Ghavami, Shaghayegh Baradaran; Rostami, Elham; Sephay, Abbas Akhavan; Shahrokh, Shabnam; Balaii, Hedieh; Aghdaei, Hamid Asadzadeh; Zali, Mohammad Reza

doi:10.1016/j.micpath.2018.01.029

Cited by 81 publications

(70 citation statements)

References 33 publications

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“…SRB and the consequent disturbance of butyrate oxidation is believed to play a key role in the pathogenesis of IBD [12]. If SRB are indeed more present in RTRs, this might also be an explanatory factor for the occurrence of any of the gastrointestinal complaints of RTRs [16], especially since the butyrate concentration in RTRs seems to be lower due to the reduced prevalence of bacteria taxa that produce butyrate [7].…”

Section: Discussionmentioning

confidence: 99%

“…Measuring breath CH 4 concentrations is a simple way to investigate the metabolism of intestinal methanogens, since no significant catabolism elsewhere in the human body has been observed [12]. The presence of M. smithii can be measured in the feces, as has previously been performed in studies investigating patients suffering from inflammatory bowel disease (IBD) [12,16].…”

Section: Introductionmentioning

confidence: 99%

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Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Knobbe

Douwes

Kremer

et al. 2020

JCM

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Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Knobbe

Douwes

Kremer

et al. 2020

JCM

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“…Our findings showed that responders had higher relative levels of abundance of the phyla Verrucomincrobia (especially the Akkermansia genus) and Euryarchaeota (especially the Methanobrevibacter genus) after FMT compared with baseline. There is evidence that Akkermansia and Methanobrevibacter are more abundant in healthy controls than in patients with bowel diseases, 40,41 and more importantly, the high abundance of Akkermansia are reported to be inversely correlated with pain reduction. 42 Therefore, these results suggest that Akkermansia and Methanobrevibacter are involved in the improvement of bowel diseases.…”

Section: Discussionmentioning

confidence: 99%

Relief of irritable bowel syndrome by fecal microbiota transplantation is associated with changes in diversity and composition of the gut microbiota

Huang

Chen

Luo

et al. 2019

J of Digest Diseases

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Objective To evaluate the efficacy and safety of fecal microbiota transplantation (FMT) for refractory irritable bowel syndrome (IBS). Methods Microbiota suspensions from feces of the donors were injected into the intestines of 30 Chinese patients with refractory IBS. Microbiota composition analysis and genomic DNA extraction of fecal samples obtained from these patients at baseline and 1 month after FMT were performed. Clinical efficacy and safety of FMT were assessed using questionnaires covering four aspects of IBS therapeutic efficacy and assessment of adverse effects during a 6‐month follow‐up. Results FMT improved IBS gastrointestinal symptoms and alleviated depression and anxiety, as shown by the improved IBS‐QOL, IBS‐SSS, GSRS, HAMA and HAMD scores at 1 month and 3 months after FMT. A total adverse event rate of FMT was 6.7% (2/30). Gut microbiota analysis revealed that FMT responders had a significantly higher Shannon diversity index before FMT than non‐responders. In addition, analysis of differences in bacterial composition before and after FMT in responders showed specific abundance of the phyla Verrucomincrobia and Euryarchaeota at 1 month after FMT. At the genus level, Methanobrevibacter and Akkermansia were the most abundant fecal microbiota 1 month after FMT compared with those before FMT. Conclusions FMT may be an effective and safe therapeutic strategy for treating IBS that achieves a sustained clinical response 3‐6 months after the first procedure. Changes in the diversity and dominant flora may contribute to its therapeutic effect.

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“…in IBD patients [33]. Methanobrevibacter smithii, an archaea methanogen, was significantly decreased in IBD patients [36]. Another study showed no change in fecal M. smithii but increased fecal Methanosphaera stadtmanae in IBD patients [37].…”

Section: Discussionmentioning

confidence: 98%

Development of an Index Score for Intestinal Inflammation-Associated Dysbiosis Using Real-World Stool Test Results

et al. 2019

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Background Gut microbiota play an important role in human health. However, the application of gut microbiome in regular clinical practice is limited by interindividual variations and complexity of test results. Hypothesis It is possible to address interindividual variation by using large data-based exploratory-pattern analysis. Methods The current study was conducted using a large data set (n = 173,221) of nonselective incoming patients' test results from a stool test. The data set included assays for the detection of 24 selected commensal microorganisms and multiple biomarkers in feces. Patients were grouped based on their levels of inflammation biomarkers such as calprotectin, eosinophil protein X, and IgA. Group mean values of biomarkers and commensal microbes were used in an exploratory-pattern analysis for association from which an index score for intestinal inflammation-associated dysbiosis (IAD) was developed. The IAD score was evaluated in one questionnaire-based study (n = 7263) and one prospective case series study (n = 122) with patients of inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease. Results We identified a microbial profile strongly associated with fecal inflammation biomarkers. Developed on the pattern of the microbial profile, the IAD score demonstrated a strong association with fecal inflammation biomarkers and was significantly different between patients with IBD and those with IBS or celiac disease. Conclusion Using real-world data, we have developed a method to predict gut dysbiosis associated with different GI disease conditions. It may help clinicians simplify the process of interpreting gut microbial status and provide gut health assessment and treatment evaluation.

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Alterations of the human gut Methanobrevibacter smithii as a biomarker for inflammatory bowel diseases

Cited by 81 publications

References 33 publications

Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Relief of irritable bowel syndrome by fecal microbiota transplantation is associated with changes in diversity and composition of the gut microbiota

Development of an Index Score for Intestinal Inflammation-Associated Dysbiosis Using Real-World Stool Test Results

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