Alterations of regulatory factors and DNA methylation pattern in thyroid cancer

Iancu, Iulia Virginia; Botezatu, Anca; Pleşa, Adriana; Huică, Irina; Fudulu, Alina; Albulescu, Adrian; Bostan, Marinela; Mihăilă, Mirela; Grancea, Camelia; Manda, Dana; Dobrescu, Ruxandra; Vlădoiu, Suzana; Anton, Gabriela; Badiu, Corin

doi:10.3233/cbm-190871

Cited by 14 publications

(6 citation statements)

References 46 publications

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“… 22 Earlier studies have already identified the role of abnormal DNA promoter methylation in different types of human cancers. 23 , 24 In our study, we noticed that the TMED2 promoter methylation level was lower as per expectations in CESC, ESCA, HNSC, and KIRC samples relative to normal controls. In contrast, we observed that TMED2 promoter methylation level was unexpectedly higher in LIHC and LUAD samples as compared to the normal controls.…”

Section: Discussionsupporting

confidence: 82%

Multi-Omics Analysis Identified TMED2 as a Shared Potential Biomarker in Six Subtypes of Human Cancer

Sial¹,

Saeed²,

Ahmad³

et al. 2021

IJGM

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Section: Discussionsupporting

confidence: 82%

Multi-Omics Analysis Identified TMED2 as a Shared Potential Biomarker in Six Subtypes of Human Cancer

Sial¹,

Saeed²,

Ahmad³

et al. 2021

IJGM

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“…The change of normal DNA methylation patterns contain DNA hypomethylation, which occurs pathologically in normally unmethylated regions of the genome, and DNA hypermethylation, which usually occurs in the CpG islands of gene promoters ( 33 ). So far, previous studies have found a correlation between DNA methylation changes and many tumors ( 34 , 35 ). In DNA methylation analysis, we found that NUF2 methylation level was lower in LUAD than in normal tissues.…”

Section: Discussionmentioning

confidence: 96%

Integrative Multi-Omics Analysis of Identified NUF2 as a Candidate Oncogene Correlates With Poor Prognosis and Immune Infiltration in Non-Small Cell Lung Cancer

Chen

Liang

et al. 2021

Front. Oncol.

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BackgroundLung cancer is one of the most common malignant tumors and the leading causes of cancer-related deaths worldwide. As a component of the nuclear division cycle 80 complex, NUF2 is a part of the conserved protein complex related to the centromere. Although the high expression of NUF2 has been reported in many different types of human cancers, the multi-omics analysis in non-small cell lung cancer (NSCLC) of NUF2 remains to be elucidated.MethodsIn this analysis, NUF2 expression difference analysis in non-small cell lung cancer was evaluated by Oncomine, TIMER, GEO, and TCGA database. And the prognosis analysis of NUF2 based on Kaplan-Meier was performed. R language was used to analyze the differential expression genes, functional annotation and protein-protein interaction (PPI). GSEA analysis of differential expression genes was also carried out. Mechanism analysis about exploring the characteristic of NUF2, multi-omics, and correlation analysis was carried out using UALCAN, cBioportal, GEPIA, TIMER, and TISIDB, respectively.ResultsThe expression of NUF2 in NSCLC, both lung adenocarcinoma (LUAD) and squamous lung cancer (LUSC), was significantly higher than that in normal tissues. The analysis of UALCAN database samples proved that NUF2 expression was connected with stage and smoking habits. Meanwhile, the overall survival curve also validated that high expression of NUF2 has a poorer prognosis in NSCLC. GO, KEGG, GSEA, subcellular location from COMPARTMENTS indicated that NUF2 may regulate the cell cycle. Correlation analysis also showed that NUF2 was mainly positively associated with cell cycle and tumor-related genes. NUF2 altered group had a poorer prognosis than unaltered group in NSCLC. Immune infiltration analysis showed that the NUF2 expression mainly have negatively correlation with immune cells and immune subtypes in LUAD and LUSC. Furthermore, quantitative PCR was used to validate the expression difference of NUF2 in LUAD and LUSC.ConclusionOur findings elucidated that NUF2 may play an important role in cell cycle, and significantly associated with tumor-related gene in NSCLC; we consider that NUF2 may be a prognostic biomarkers in NSCLC.

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“…The samples stored at 4 °C until analyzed by flow cytometry. A minimum of 20,000 events for each sample were collected using a FACS CantoII flow cytometer and ModFIT software (BectonDickinson) and used to determine the cell cycle phase distribution after debris exclusion [ 58 , 59 ].…”

Section: Methodsmentioning

confidence: 99%

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

et al. 2020

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Natural compounds can modulate all three major phases of carcinogenesis. The role of the natural compounds such as resveratrol (RSV) and curcumin (CRM) in modulation of anticancer potential of platinum-based drugs (CisPt) is still a topic of considerable debate. In order to enhance head and neck cancer (HNSCC) cells’ sensitivity to the cytotoxic effects of CisPt combined treatments with RSV or CRM were used. The study aim was to evaluate how the RSV or CRM associated to CisPt treatment modulated some cellular processes such as proliferation, P21 gene expression, apoptotic process, and cell cycle development in HNSCC tumor cell line (PE/CA-PJ49) compared to a normal cell line (HUVEC). The results showed that RSV or CRM treatment affected the viability of tumor cells more than normal cells. These natural compounds act against proliferation and sustain the effects of cisplatin by cell cycle arrest, induction of apoptosis and amplification of P21 expression in tumor cells. In conclusion, using RSV or CRM as adjuvants in CisPt therapy might have a beneficial effect by supporting the effects induced by CisPt.

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Alterations of regulatory factors and DNA methylation pattern in thyroid cancer

Cited by 14 publications

References 46 publications

Multi-Omics Analysis Identified TMED2 as a Shared Potential Biomarker in Six Subtypes of Human Cancer

Multi-Omics Analysis Identified TMED2 as a Shared Potential Biomarker in Six Subtypes of Human Cancer

Integrative Multi-Omics Analysis of Identified NUF2 as a Candidate Oncogene Correlates With Poor Prognosis and Immune Infiltration in Non-Small Cell Lung Cancer

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

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