Alterations of peroxisome proliferator-activated receptor γ and monocyte chemoattractant protein 1 gene expression in the nitrofen-induced hypoplastic lung

Evidence for decreased lipofibroblast expression in hypoplastic rat lungs with congenital diaphragmatic hernia

“…Immunofluorescence double staining confirmed a decreased LIF expression in the alveolar interstitium of CDH-associated PH with markedly reduced lipid inclusions, indicating an impaired LIF functioning in nitrofen-exposed rat lungs during the critical time period of fetal alveolarization and alveolar maturation. Recent studies from our laboratory have provided additional evidence for this theory by showing significant downregulation of further regulatory components of the ADRP signaling pathway in fetal rat lungs with CDH-associated PH during late gestation, while their relative mRNA expression peaks normally in control lungs [26][27][28].…”

Disruption of the Bone Morphogenetic Protein Receptor 2 Pathway in Nitrofen‐Induced Congenital Diaphragmatic Hernia

“…Furthermore, BMPR2 signaling has been suggested to prevent extensive vascular remodeling induced by chemokines (such as MCP-1) (Hagen et al, 2007;Reynolds et al, 2007), thus ensuring normal vasculogenesis. The BMPR2 target proteins PDGF-BB and MCP-1 have been reported to be significantly increased in several animal models of PH including nitrofen-induced CDH (Chang et al, 2012;Gosemann et al, 2012). It is well known that BMPR2 signals through at least two alternate pathways, SMAD and p38 MAPK (Hagen et al, 2007;Rudarakanchana et al, 2002).…”

“…Administration of the herbicide nitrofen (2,4-dichloro-p-nitrophenyl ether) to pregnant rats on day 9 (D9) of gestation induces PH and diaphragmatic defects in the offspring with striking similarities of the pathologies observed in human CDH (Beurskens et al, 2007;Corbett et al, 2012;Luong et al, 2011). It has been recently reported that the BMPR2 target proteins PDGF-BB and MCP-1 are significantly increased in nitrofeninduced CDH lungs (Chang et al, 2012;Gosemann et al, 2012).…”

Prenatal treatment with rosiglitazone attenuates vascular remodeling and pulmonary monocyte influx in experimental congenital diaphragmatic hernia

“…Presence of spermatozoids in the vaginal smear was considered as proof of pregnancy and pregnant females were randomly divided into 2 groups (“control” and “CDH”). On gestational day 9 (D9), 100 mg of nitrofen (2,4-dichloro- p -nitrophenyl ether, WAKO Chemicals, Osaka, Japan) dissolved in 1 ml olive oil was administered intragastrically to animals of the CDH group, as previously described [ 15 ]. Animals assigned to the control group received vehicle only.…”